Question 1

A DNA molecule used to carry a foreign gene into a host organism is called a

Accepted Answer

A) plasmid. 
B) vector. 
C) probe. 
D) blot. 
A) plasmid. 
B) vector. 
C) probe. 
D) blot. B

Question 2

When a eukaryotic gene is cloned into a bacterium,the advantage of a complementary DNA (cDNA)gene being used instead of fragments of genomic DNA is that

Accepted Answer

A) the promoter and terminator are found in the cDNA gene but not in the genomic fragment. 
B) the introns have been removed from the cDNA gene but not from the genomic fragment. 
C) the cDNA is made with the nucleotides found in the prokaryote but not in the eukaryote. 
D) there is no advantage to using a cDNA gene rather than a genomic fragment. 
A) the promoter and terminator are found in the cDNA gene but not in the genomic fragment. 
B) the introns have been removed from the cDNA gene but not from the genomic fragment. 
C) the cDNA is made with the nucleotides found in the prokaryote but not in the eukaryote. 
D) there is no advantage to using a cDNA gene rather than a genomic fragment. B

Question 3

Regardless of the exact approach taken to recombinant DNA technology,one of the keys to successful cloning is choosing the right vector.

Accepted Answer

A) True 
 B)False  True

Question 4

Recombinant DNA technology does not rely on which of the following enzymes?

Accepted Answer

A) restriction endonucleases 
B) RNA methylase 
C) DNA ligase 
D) reverse transcriptase 
A) restriction endonucleases 
B) RNA methylase 
C) DNA ligase 
D) reverse transcriptase

Question 5

Complementary DNA (cDNA)probes are produced using

Accepted Answer

A) restriction endonucleases. 
B) RNA polymerase. 
C) DNA ligase. 
D) reverse transcriptase. 
A) restriction endonucleases. 
B) RNA polymerase. 
C) DNA ligase. 
D) reverse transcriptase.

Question 6

When a eukaryotic gene is expressed in a bacterium,the eukaryotic regulatory sequences should be maintained in order to achieve maximum expression of the gene.

Accepted Answer

A) True 
 B)False

Question 7

In _____________,cells are mixed with recombinant DNA and exposed to a brief pulse of high-voltage electricity to cause the membrane to become permeable and allow the uptake of DNA from its environment.

Accepted Answer

The answer of In _____________,cells are mixed with recombinant DNA...

Question 8

Plasmid vectors often contain __________ genes that can be used to screen for recombinants.

Accepted Answer

A) metabolic activation 
B) antibiotic resistance 
C) insertion sequence 
D) promoter/operator 
A) metabolic activation 
B) antibiotic resistance 
C) insertion sequence 
D) promoter/operator

Question 9

A(n)__________ __________ is a piece of DNA with all of the features necessary for chromosomal replication and which can carry large (up to 1000 kb)pieces of foreign DNA into a host organism.

Accepted Answer

artificial

Question 10

Genetic engineering methods have been used to produce vaccines.

Accepted Answer

A) True 
 B)False

Question 11

The enzyme reverse transcriptase was discovered by

Accepted Answer

A) Arber and Smith. 
B) Jackson,Symons,and Berg. 
C) Boyer and Cohen. 
D) Temin and Baltimore. 
A) Arber and Smith. 
B) Jackson,Symons,and Berg. 
C) Boyer and Cohen. 
D) Temin and Baltimore.

Question 12

Restriction endonucleases in bacteria may have evolved in order to

Accepted Answer

A) carry out natural genetic engineering. 
B) protect the bacteria from infection by viruses. 
C) use nucleic acids as a food (energy)source. 
D) all of the choices 
A) carry out natural genetic engineering. 
B) protect the bacteria from infection by viruses. 
C) use nucleic acids as a food (energy)source. 
D) all of the choices

Question 13

In order to express eukaryotic genes in a bacterium,the __________ must first be removed.

Accepted Answer

A) introns 
B) exons 
C) enhancers 
D) 3' poly A sequence 
A) introns 
B) exons 
C) enhancers 
D) 3' poly A sequence

Question 14

A PCR procedure that allows a determination of the amount of a particular DNA fragment that is present in a sample is called

Accepted Answer

A) quantitative PCR. 
B) analytical PCR. 
C) real-time PCR. 
D) reverse PCR. 
A) quantitative PCR. 
B) analytical PCR. 
C) real-time PCR. 
D) reverse PCR.

Question 15

One of the major advantages to using plasmids as cloning vectors is that very high copy numbers can be achieved with many types of plasmid vectors.

Accepted Answer

A) True 
 B)False

Question 16

Transposons are frequently used as cloning vectors.

Accepted Answer

A) True 
 B)False

Question 17

Which of the following types of cloning vector can carry the largest amount of foreign DNA?

Accepted Answer

A) bacterial artificial chromosome. 
B) bacteriophage. 
C) cosmid. 
D) plasmid. 
A) bacterial artificial chromosome. 
B) bacteriophage. 
C) cosmid. 
D) plasmid.

Question 18

Plasmid cloning vector DNA is usually introduced into bacterial hosts by

Accepted Answer

A) ligation. 
B) transformation. 
C) transduction. 
D) plasmolysis. 
A) ligation. 
B) transformation. 
C) transduction. 
D) plasmolysis.

Question 19

The three steps that take place in each cycle during PCR occur in which order?

Accepted Answer

A) DNA annealing,denaturation,and synthesis. 
B) DNA denaturation,annealing,and synthesis. 
C) DNA synthesis,denaturation,and annealing. 
D) none of the above. 
A) DNA annealing,denaturation,and synthesis. 
B) DNA denaturation,annealing,and synthesis. 
C) DNA synthesis,denaturation,and annealing. 
D) none of the above.

Question 20

A (n)__________ vector contains promoters that result in high-level transcription of the gene cloned within a multicloning site.

Accepted Answer

A) shuttle 
B) chimeric 
C) expression 
D) phage 
A) shuttle 
B) chimeric 
C) expression 
D) phage

Exam 17: Recombinant Dna Technology

A DNA molecule used to carry a foreign gene into a host organism is called a

When a eukaryotic gene is cloned into a bacterium,the advantage of a complementary DNA (cDNA)gene being used instead of fragments of genomic DNA is that

Regardless of the exact approach taken to recombinant DNA technology,one of the keys to successful cloning is choosing the right vector.

Recombinant DNA technology does not rely on which of the following enzymes?

Complementary DNA (cDNA)probes are produced using

When a eukaryotic gene is expressed in a bacterium,the eukaryotic regulatory sequences should be maintained in order to achieve maximum expression of the gene.

In _____________,cells are mixed with recombinant DNA and exposed to a brief pulse of high-voltage electricity to cause the membrane to become permeable and allow the uptake of DNA from its environment.

Plasmid vectors often contain __________ genes that can be used to screen for recombinants.

A(n)__________ __________ is a piece of DNA with all of the features necessary for chromosomal replication and which can carry large (up to 1000 kb)pieces of foreign DNA into a host organism.

Genetic engineering methods have been used to produce vaccines.

The enzyme reverse transcriptase was discovered by

Restriction endonucleases in bacteria may have evolved in order to

In order to express eukaryotic genes in a bacterium,the __________ must first be removed.

A PCR procedure that allows a determination of the amount of a particular DNA fragment that is present in a sample is called

One of the major advantages to using plasmids as cloning vectors is that very high copy numbers can be achieved with many types of plasmid vectors.

Transposons are frequently used as cloning vectors.

Which of the following types of cloning vector can carry the largest amount of foreign DNA?

Plasmid cloning vector DNA is usually introduced into bacterial hosts by

The three steps that take place in each cycle during PCR occur in which order?

A (n)__________ vector contains promoters that result in high-level transcription of the gene cloned within a multicloning site.

The Evolution of Microorganisms and Microbiology

Microscopy

Bacterial Cell Structure

Archaeal Cell Structure

Eukaryotic Cell Structure

Viruses and Other Acellular Infectious Agents

Microbial Growth

Control of Microorganisms in the Environment

Antimicrobial Chemotherapy

Introduction to Metabolism

Catabolism: Energy Release and Conservation

Anabolism: the Use of Energy in Biosynthesis

Bacterial Genome Replication and Expression

Regulation of Bacterial Cellular Processes

Eukaryotic and Archaeal Genome Replication and Expression

Mechanisms of Genetic Variation

Microbial Genomics

Microbial Taxonomy and the Evolution of Diversity

The Archaea

The Deinococci,mollicutes,and Nonproteobacterial Gram-Negative Bacteria

The Proteobacteria

Firmicutes: the Low G 1 C Gram-Positive Bacteria

Actinobacteria: the High G 1 C Gram-Positive Bacteria

The Protists

The Fungi Eumycota

Viruses

Biogeochemical Cycling and Global Climate Change

Methods in Microbial Ecology

Microorganisms in Marine and Freshwater Ecosystems

Microorganisms in Terrestrial Ecosystems

Microbial Interactions

Innate Host Resistance

Adaptive Immunity

Pathogenicity and Infection

Clinical Microbiology and Immunology

Epidemiology and Public Health Microbiology

Human Diseases Caused by Viruses and Prions

Human Diseases Caused by Bacteria

Human Diseases Caused by Fungi and Protists

Microbiology of Food

Biotechnology and Industrial Microbiology

Applied Environmental Microbiology

Filters

A(n) is a piece of DNA with all of the features necessary for chromosomal replication and which can carry large (up to 1000 kb)pieces of foreign DNA into a host organism.