Question 1

Restriction endonucleases are produced by

Accepted Answer

A) fungi. 
B) bacteria. 
C) protozoa. 
D) plants. 
E) all of the choices 
A) fungi. 
B) bacteria. 
C) protozoa. 
D) plants. 
E) all of the choices

Question 2

The thermostable enzyme most commonly used in PCR is reverse transcriptase.

Accepted Answer

A) True 
 B)False

Question 3

Movement of charged molecules in an electrical field,which is used to separate nucleic acid fragments for recombinant DNA work,is called

Accepted Answer

A) iontophoresis. 
B) nucleophoresis. 
C) electrophoresis. 
D) plasmaphoresis. 
A) iontophoresis. 
B) nucleophoresis. 
C) electrophoresis. 
D) plasmaphoresis.

Question 4

Electroporation is commonly used to introduce recombinant DNA molecules into cells.

Accepted Answer

A) True 
 B)False

Question 5

The most frequently chosen prokaryotic host in cloning techniques is

Accepted Answer

A) Saccharomyces cerevisiae. 
B) Bacillus subtilis. 
C) Staphylococcus aureus. 
D) Escherichia coli. 
A) Saccharomyces cerevisiae. 
B) Bacillus subtilis. 
C) Staphylococcus aureus. 
D) Escherichia coli.

Question 6

Antibiotics incorporated into the culture medium can

Accepted Answer

A) select against organisms that have not incorporated the plasmid. 
B) select against organisms that have incorporated a plasmid not containing the desired gene. 
C) enhance production of recombinant proteins. 
D) select against organisms that have not incorporated the plasmid and select against organisms that have incorporated a plasmid not containing the desired gene 
A) select against organisms that have not incorporated the plasmid. 
B) select against organisms that have incorporated a plasmid not containing the desired gene. 
C) enhance production of recombinant proteins. 
D) select against organisms that have not incorporated the plasmid and select against organisms that have incorporated a plasmid not containing the desired gene

Question 7

A __________ is a DNA molecule used in hybridization reactions to detect the presence of a particular gene in separated DNA fragments.

Accepted Answer

A) plasmid 
B) vector 
C) probe 
D) blot 
A) plasmid 
B) vector 
C) probe 
D) blot

Question 8

The production of large quantities of a particular DNA sequence is known as gene __________________.

Accepted Answer

The answer of The production of large quantities of a...

Question 9

Some plasmid vectors have incorporated the regulatory sequences of the lactose operon so that the expression of the recombinant gene can be induced at the appropriate time.

Accepted Answer

A) True 
 B)False

Question 10

___________ is a bacterial plasmid vector.

Accepted Answer

A) Lambda 
B) T4 DNA ligase 
C) pUC19 
D) SV40 
A) Lambda 
B) T4 DNA ligase 
C) pUC19 
D) SV40

Question 11

Cloning a gene involves all of the following except

Accepted Answer

A) isolating the fragment of DNA containing the desired gene. 
B) insertion of the gene into an appropriate vector. 
C) expression of the vector and the gene in a cell-free environment. 
D) introducing ligated DNA into 
E) coli cells. 
A) isolating the fragment of DNA containing the desired gene. 
B) insertion of the gene into an appropriate vector. 
C) expression of the vector and the gene in a cell-free environment. 
D) introducing ligated DNA into 
E) coli cells.

Question 12

The PCR method was developed by

Accepted Answer

A) Boyer. 
B) Mullis. 
C) Cohen. 
D) Sanger. 
A) Boyer. 
B) Mullis. 
C) Cohen. 
D) Sanger.

Question 13

A genomic __________ is a sufficiently large collection of recombinant DNA molecules in which the inserted sequences together represent the entire genome of an organism.

Accepted Answer

The answer of A genomic __________ is a sufficiently large...

Question 14

Cosmids are so named because they can be used to express foreign genes in a variety of different hosts.

Accepted Answer

A) True 
 B)False

Question 15

Which of the following best describes the basis for separation of DNA fragments during agarose gel electrophoresis?

Accepted Answer

A) The fragments with the highest percentage of G and C will migrate fastest. 
B) The fragments with the highest percentage of A and T will migrate fastest. 
C) The largest fragments will migrate fastest. 
D) The smallest fragments will migrate fastest. 
A) The fragments with the highest percentage of G and C will migrate fastest. 
B) The fragments with the highest percentage of A and T will migrate fastest. 
C) The largest fragments will migrate fastest. 
D) The smallest fragments will migrate fastest.

Question 16

An enzyme that cleaves internal phosphodiester bonds of a DNA molecule is a (n)

Accepted Answer

A) exonuclease. 
B) endonuclease. 
C) ligase. 
D) methylase. 
A) exonuclease. 
B) endonuclease. 
C) ligase. 
D) methylase.

Question 17

Cosmids are plasmids that can be packaged into capsids of the bacteriophage lambda; therefore,they can be transmitted like phages,but they can exist and replicate in a cell like plasmids.

Accepted Answer

A) True 
 B)False

Question 18

The Southern blotting technique for transferring DNA from an agarose gel to a piece of nitrocellulose membrane is named for
E.M.Southern,the person who developed the procedure.

Accepted Answer

A) True 
 B)False

Question 19

Which of the following is true about restriction endonucleases?

Accepted Answer

A) They make a blunt cut on the two DNA strands so that there are no single-strand regions. 
B) They make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme. 
C) Some make a blunt cut on the two DNA strands so that there are no single-strand regions and some make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme. 
D) Depending on the incubation conditions,the same enzyme can either make a blunt cut on the two DNA strands so that there are no single-strand regions OR make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme. 
A) They make a blunt cut on the two DNA strands so that there are no single-strand regions. 
B) They make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme. 
C) Some make a blunt cut on the two DNA strands so that there are no single-strand regions and some make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme. 
D) Depending on the incubation conditions,the same enzyme can either make a blunt cut on the two DNA strands so that there are no single-strand regions OR make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.

Question 20

Which of the following is not true of cloning vectors?

Accepted Answer

A) They usually contain multicloning sites or polylinkers. 
B) They contain at least two replication origins. 
C) They can be replicated within an appropriate host. 
D) All of these are true of cloning vectors. 
A) They usually contain multicloning sites or polylinkers. 
B) They contain at least two replication origins. 
C) They can be replicated within an appropriate host. 
D) All of these are true of cloning vectors.

Exam 17: Recombinant Dna Technology

Restriction endonucleases are produced by

The thermostable enzyme most commonly used in PCR is reverse transcriptase.

Movement of charged molecules in an electrical field,which is used to separate nucleic acid fragments for recombinant DNA work,is called

Electroporation is commonly used to introduce recombinant DNA molecules into cells.

The most frequently chosen prokaryotic host in cloning techniques is

Antibiotics incorporated into the culture medium can

A __________ is a DNA molecule used in hybridization reactions to detect the presence of a particular gene in separated DNA fragments.

The production of large quantities of a particular DNA sequence is known as gene __________________.

Some plasmid vectors have incorporated the regulatory sequences of the lactose operon so that the expression of the recombinant gene can be induced at the appropriate time.

___________ is a bacterial plasmid vector.

Cloning a gene involves all of the following except

The PCR method was developed by

A genomic __________ is a sufficiently large collection of recombinant DNA molecules in which the inserted sequences together represent the entire genome of an organism.

Cosmids are so named because they can be used to express foreign genes in a variety of different hosts.

Which of the following best describes the basis for separation of DNA fragments during agarose gel electrophoresis?

An enzyme that cleaves internal phosphodiester bonds of a DNA molecule is a (n)

Cosmids are plasmids that can be packaged into capsids of the bacteriophage lambda; therefore,they can be transmitted like phages,but they can exist and replicate in a cell like plasmids.

The Southern blotting technique for transferring DNA from an agarose gel to a piece of nitrocellulose membrane is named for E.M.Southern,the person who developed the procedure.

Which of the following is true about restriction endonucleases?

Which of the following is not true of cloning vectors?

The Evolution of Microorganisms and Microbiology

Microscopy

Bacterial Cell Structure

Archaeal Cell Structure

Eukaryotic Cell Structure

Viruses and Other Acellular Infectious Agents

Microbial Growth

Control of Microorganisms in the Environment

Antimicrobial Chemotherapy

Introduction to Metabolism

Catabolism: Energy Release and Conservation

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Bacterial Genome Replication and Expression

Regulation of Bacterial Cellular Processes

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Mechanisms of Genetic Variation

Microbial Genomics

Microbial Taxonomy and the Evolution of Diversity

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Clinical Microbiology and Immunology

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Human Diseases Caused by Viruses and Prions

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