Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

A differential interference contrast microscope uses dyes to give colored, three-dimensional images.

Which type of microscope does not use light in forming the specimen image?

Humans can see objects as small as 0.1 mm. Leeuwenhoek's best microscope could magnify objects to 300X. Which of the following would be invisible using Leeuwenhoek's microscope?

Differential media result in which of the following observable characteristics?

A microbiologist must culture a patient's feces for intestinal pathogens. Which of the following components would likely be present in a selective medium for this purpose?

Scattering of peripheral light rays through the glass slide under very high magnification can be prevented by ______.

The Gram staining procedure is best described as a ______ staining technique.

Which of the following parts of the microscope magnifies the specimen to produce the virtual image of the specimen?

The Five I's of studying microorganisms include all of the following except ______.

Which type of medium distinguishes different types of microorganisms based on an observable change in the colonies or in the medium?

Which of the following is not an inoculating tool?

Which type of microscope cannot image live specimens?

Eosin-methylene blue agar, or EMB, is a commonly used bacteriological medium for growing gram- negative bacteria from complex environments. It contains two dyes, eosin and methylene blue, as well as the sugar lactose. Eosin is a pH indicator that changes color when the medium is acidic, while methylene blue inhibits the growth of most gram-positive bacteria. If an organism consumes lactose, acid will be produced, causing the bacterial colonies to change color. based upon this description, this medium is ______.

Your microscope has a resolving power of 0.15 μm. The specimen that you will be observing has bacterial cocci the size of 0.1 μm in diameter. Which of the following statements is correct?

Which type of microscope achieves the greatest resolution and highest magnification?

A selective medium contains one or more substances that inhibit growth of some microbes to facilitate the growth of other desired microbes.

A reducing medium is used to culture ______ organisms.

A textbook uses Paramecium to illustrate several types of light microscopy. Each image is magnified 230X and measures about 4 cm. The actual size of a Paramecium is about ______.

Agar is a complex polysaccharide that comes from a/an ______.

Fixed smears of specimens are required to perform the Gram stain or the endospore stain.