Question 1

All of the following are examples of different types of microbiological media except ______.

Accepted Answer

A)  agar 
B)  Petri dish 
C)  broth 
D)  semisolid 
E)  enriched 
A)  agar 
B)  Petri dish 
C)  broth 
D)  semisolid 
E)  enriched

Question 2

The procedures for culturing a microorganism require the use of a microscope.

Accepted Answer

A) True 
 B)False

Question 3

The bacterium Neisseria gonorrhoeae causes the sexually-transmitted infection gonorrhea. Although it can be isolated from the vagina, there are numerous normal biota in that location as well. To generate a pure culture of this pathogenic bacterium, the best choice is a/an ______.

Accepted Answer

A)  selective medium 
B)  differential medium 
C)  liquid medium 
D)  enrichment medium 
A)  selective medium 
B)  differential medium 
C)  liquid medium 
D)  enrichment medium

Question 4

A reducing medium contains _______.

Accepted Answer

A)  sugars that can be fermented 
B)  extra oxygen 
C)  hemoglobin, vitamins, or other growth factors 
D)  substances that remove oxygen 
E)  inhibiting agents 
A)  sugars that can be fermented 
B)  extra oxygen 
C)  hemoglobin, vitamins, or other growth factors 
D)  substances that remove oxygen 
E)  inhibiting agents

Question 5

Which of the following parts of a microscope magniﬁes the specimen to produce a real image of the specimen?

Accepted Answer

A)  Nosepiece 
B)  Objective lens 
C)  Ocular lens 
D)  Body 
E)  Condenser 
A)  Nosepiece 
B)  Objective lens 
C)  Ocular lens 
D)  Body 
E)  Condenser

Question 6

The wavelength of light used and the numerical aperture governs ______.

Accepted Answer

A)  size of the ﬁeld 
B)  illumination 
C)  resolution 
D)  contrast 
E)  magniﬁcation 
A)  size of the ﬁeld 
B)  illumination 
C)  resolution 
D)  contrast 
E)  magniﬁcation

Question 7

Which of the following is essential for the development of discrete, isolated colonies?

Accepted Answer

A)  Solid medium 
B)  Selective medium 
C)  Broth medium 
D)  Differential medium 
E)  Assay medium 
A)  Solid medium 
B)  Selective medium 
C)  Broth medium 
D)  Differential medium 
E)  Assay medium

Question 8

If a microbiologist is studying a specimen at a total magniﬁcation of 950X, what is the magnifying power of the objective lens if the ocular lens is 10X?

Accepted Answer

A)  940X 
B)  95X 
C)  950X 
D)  9500X 
A)  940X 
B)  95X 
C)  950X 
D)  9500X

Question 9

Mannitol salt agar is selective for organisms in which bacterial genus?

Accepted Answer

A)  Escherichia 
B)  Salmonella 
C)  Neisseria 
D)  Staphylococcus 
E)  Streptococcus 
A)  Escherichia 
B)  Salmonella 
C)  Neisseria 
D)  Staphylococcus 
E)  Streptococcus

Question 10

Bacteria that require special growth factors and complex nutrients are termed ______.

Accepted Answer

A)  fermentative 
B)  aerobic 
C)  hemolytic 
D)  anaerobic 
E)  fastidious 
A)  fermentative 
B)  aerobic 
C)  hemolytic 
D)  anaerobic 
E)  fastidious

Question 11

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except ______.

Accepted Answer

A)  2.0 µm 
B)  0.1 µm 
C)  0.2 µm 
D)  0.2 mm 
A)  2.0 µm 
B)  0.1 µm 
C)  0.2 µm 
D)  0.2 mm

Question 12

Cells of Staphylococcus aureus are cocci measuring about 1 ?m in diameter. In a textbook image of S. aureus, each cell measures about 1 cm. The magni?cation of the image on the page is ______.

Accepted Answer

A)  10,000X 
B)  1,000X 
C)  100X 
D)  0.01X 
E)  0.001X 
F)0.0001X 
A)  10,000X 
B)  1,000X 
C)  100X 
D)  0.01X 
E)  0.001X 
F)0.0001X

Question 13

Which of the following is produced by adding 1% to 5% agar to nutrient broth that is then boiled and cooled?

Accepted Answer

A)  A pure culture 
B)  A mixed culture 
C)  A liquid medium 
D)  A contaminated medium 
E)  A solid medium 
A)  A pure culture 
B)  A mixed culture 
C)  A liquid medium 
D)  A contaminated medium 
E)  A solid medium

Question 14

A common medium for culturing fastidious bacteria is ______.

Accepted Answer

A)  MacConkey medium 
B)  trypticase soy agar 
C)  blood agar 
D)  mannitol salt agar 
E)  reducing medium 
A)  MacConkey medium 
B)  trypticase soy agar 
C)  blood agar 
D)  mannitol salt agar 
E)  reducing medium

Question 15

A microbiologist inoculates Staphylococcus aureus into a culture medium. Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture. What is the most likely explanation?

Accepted Answer

A)  The culture was contaminated. 
B)  The culture medium was selective. 
C)  The incubation temperature was incorrect. 
D)  The culture medium was differential. 
E)  The microbiologist used too much inoculum. 
A)  The culture was contaminated. 
B)  The culture medium was selective. 
C)  The incubation temperature was incorrect. 
D)  The culture medium was differential. 
E)  The microbiologist used too much inoculum.

Question 16

A nutrient medium that has all of its chemical components identiﬁed and their precise concentrations known and reproducible is termed ______.

Accepted Answer

A)  enriched 
B)  deﬁned 
C)  reducing 
D)  complex 
A)  enriched 
B)  deﬁned 
C)  reducing 
D)  complex

Question 17

Robert Koch and his colleagues ﬁrst used agar in bacteriological media. Why was this ingredient an improvement on previous materials used to culture bacteria?

Accepted Answer

A)  Agar was less expensive than the nutrients that Koch was previously using in the lab. 
B)  Agar can convert from liquid to solid form, and back again, very easily. 
C)  Bacteria growing on the solidiﬁed medium did not degrade or break down the agar. 
D)  Agar is a complete source of nutrients for most bacteria. 
A)  Agar was less expensive than the nutrients that Koch was previously using in the lab. 
B)  Agar can convert from liquid to solid form, and back again, very easily. 
C)  Bacteria growing on the solidiﬁed medium did not degrade or break down the agar. 
D)  Agar is a complete source of nutrients for most bacteria.

Question 18

Which type of medium would be the best choice when shipping a sample of bacteria from a clinic to a laboratory for testing?

Accepted Answer

A)  Blood agar 
B)  Transport medium 
C)  Eosin-methylene blue agar 
D)  Thioglycolate medium 
A)  Blood agar 
B)  Transport medium 
C)  Eosin-methylene blue agar 
D)  Thioglycolate medium

Question 19

Which type of microscope shows cells against a white background?

Accepted Answer

A)  Electron 
B)  Bright-ﬁeld 
C)  Confocal 
D)  Phase-contrast 
E)  Fluorescence 
A)  Electron 
B)  Bright-ﬁeld 
C)  Confocal 
D)  Phase-contrast 
E)  Fluorescence

Question 20

Bacterial cultures are easily identiﬁed from their microscopic appearance.

Accepted Answer

A) True 
 B)False

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

All of the following are examples of different types of microbiological media except ______.

The procedures for culturing a microorganism require the use of a microscope.

The bacterium Neisseria gonorrhoeae causes the sexually-transmitted infection gonorrhea. Although it can be isolated from the vagina, there are numerous normal biota in that location as well. To generate a pure culture of this pathogenic bacterium, the best choice is a/an ______.

A reducing medium contains _______.

Which of the following parts of a microscope magniﬁes the specimen to produce a real image of the specimen?

The wavelength of light used and the numerical aperture governs ______.

Which of the following is essential for the development of discrete, isolated colonies?

If a microbiologist is studying a specimen at a total magniﬁcation of 950X, what is the magnifying power of the objective lens if the ocular lens is 10X?

Mannitol salt agar is selective for organisms in which bacterial genus?

Bacteria that require special growth factors and complex nutrients are termed ______.

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except ______.

Cells of Staphylococcus aureus are cocci measuring about 1 ?m in diameter. In a textbook image of S. aureus, each cell measures about 1 cm. The magni?cation of the image on the page is ______.

Which of the following is produced by adding 1% to 5% agar to nutrient broth that is then boiled and cooled?

A common medium for culturing fastidious bacteria is ______.

A microbiologist inoculates Staphylococcus aureus into a culture medium. Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture. What is the most likely explanation?

A nutrient medium that has all of its chemical components identiﬁed and their precise concentrations known and reproducible is termed ______.

Robert Koch and his colleagues ﬁrst used agar in bacteriological media. Why was this ingredient an improvement on previous materials used to culture bacteria?

Which type of medium would be the best choice when shipping a sample of bacteria from a clinic to a laboratory for testing?

Which type of microscope shows cells against a white background?

Bacterial cultures are easily identiﬁed from their microscopic appearance.

The Main Themes of Microbiology

The Chemistry of Biology

Bacteria and Archaea

Eukaryotic Cells and Microorganisms

Viruses and Prions

Microbial Nutrition and Growth

Microbial Metabolism: the Chemical Crossroads of Life

Microbial Genetics

Genetic Analysis and Genetic Engineering

Physical and Chemical Control of Microbes

Antimicrobial Treatment

Microbe-Human Interactions: Health and Disease

Host Defenses I: Overview and Nonspecific Defenses

Host Defenses Ii: Specific Immunity and Immunization

Disorders in Immunity

Diagnosing Infections

Infectious Diseases Affecting the Skin and Eyes

Infectious Diseases Affecting the Nervous System

Infectious Diseases Affecting the Cardiovascular and Lymphatic Systems

Infectious Diseases Affecting the Respiratory System

Infectious Diseases Affecting the Gastrointestinal Tract

Infectious Diseases Affecting the Genitourinary System

Microbes and the Environment

Applied Microbiology and Food and Water Safety

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