Question 1

The world's first genetically engineered pharmaceutical product was ______.

Accepted Answer

A)  humulin 
B)  HGH 
C)  SCID 
D)  RFLP 
A)  humulin 
B)  HGH 
C)  SCID 
D)  RFLP

Question 2

Please read the following scenario then answer the following question(s).
Molecular biologists have perfected DNA fingerprinting so that it is possible to use the technique to provide evidence to solve crimes and even identify a child's parents. Recently, a U.S. immigrant asked the U.S. Citizenship and Immigration Services for permission to have her young daughter who was living with grandparents in their homeland join her.Her request was denied because there was an apparent mix-up with the child's birth certificate and it could not be used as proof of maternity. Proof is required in cases such as this. The mother requested DNA fingerprinting to make her case. Samples of DNA were taken from the mother (Mom) and daughter (D1) as well as from another daughter (D2) and a son (S) living in the United States with her. Tandem repeat analysis was run on the four samples, and the results are shown here.
  
-The short tandem repeat analysis ______.

Accepted Answer

A)  compares markers on the sex chromosomes 
B)  compares repetitive DNA sequences from different individuals 
C)  does not require DNA amplification 
D)  checks for purine and pyrimidine ratios 
A)  compares markers on the sex chromosomes 
B)  compares repetitive DNA sequences from different individuals 
C)  does not require DNA amplification 
D)  checks for purine and pyrimidine ratios

Question 3

The study of the full protein sets that genomes encode is _____.

Accepted Answer

A)  proteomics 
B)  genomics 
C)  gene therapy 
D)  gene cloning 
A)  proteomics 
B)  genomics 
C)  gene therapy 
D)  gene cloning

Question 4

"Sticky ends" are ______.

Accepted Answer

A)  single-stranded ends of fragments of double-stranded DNA 
B)  regions of double-stranded DNA that can be cut by a restriction enzyme 
C)  the site on chromosomes where the centromere is found 
D)  telomeres 
A)  single-stranded ends of fragments of double-stranded DNA 
B)  regions of double-stranded DNA that can be cut by a restriction enzyme 
C)  the site on chromosomes where the centromere is found 
D)  telomeres

Question 5

Gel electrophoresis separates DNA fragments on the basis of differences in their ______.

Accepted Answer

A)  A:C ratio 
B)  length 
C)  G:T ratio 
D)  pH 
A)  A:C ratio 
B)  length 
C)  G:T ratio 
D)  pH

Question 6

Of these steps,which one occurs earliest in the process of producing recombinant DNA?

Accepted Answer

A)  Human DNA fragments are mixed with the cut plasmids. 
B)  The same restriction enzyme is used to isolate the gene of interest and to cut the plasmid DNA. 
C)  The recombinant plasmids are mixed with bacteria. 
D)  Bacteria carrying recombinant plasmids are cloned. 
A)  Human DNA fragments are mixed with the cut plasmids. 
B)  The same restriction enzyme is used to isolate the gene of interest and to cut the plasmid DNA. 
C)  The recombinant plasmids are mixed with bacteria. 
D)  Bacteria carrying recombinant plasmids are cloned.

Question 7

Of the following,which is the last step in the production of a recombinant DNA plasmid?

Accepted Answer

A)  cloning 
B)  using DNA ligase to join DNA fragments 
C)  transduction 
D)  allowing the reproduction of the bacterium bearing the recombinant plasmid 
A)  cloning 
B)  using DNA ligase to join DNA fragments 
C)  transduction 
D)  allowing the reproduction of the bacterium bearing the recombinant plasmid

Question 8

Which of these is a genetically modified organism?

Accepted Answer

A)  an organism carrying a gene that was acquired by artificial means 
B)  the first organism in which a particular mutation has appeared 
C)  a cloned organism carrying two different alleles 
D)  an organism that gestated in an artificial womb 
A)  an organism carrying a gene that was acquired by artificial means 
B)  the first organism in which a particular mutation has appeared 
C)  a cloned organism carrying two different alleles 
D)  an organism that gestated in an artificial womb

Question 9

Cutting DNA with a particular restriction enzyme produces DNA fragments that can be separated by ______.

Accepted Answer

A)  gel electrophoresis 
B)  enzymes 
C)  recombinant DNA 
D)  plasmids 
A)  gel electrophoresis 
B)  enzymes 
C)  recombinant DNA 
D)  plasmids

Question 10

The scientific field that studies complete sets of genes is called ______.

Accepted Answer

A)  genomics 
B)  recombinant DNA 
C)  proteomics 
D)  genetic engineering 
A)  genomics 
B)  recombinant DNA 
C)  proteomics 
D)  genetic engineering

Question 11

The following figure shows that gel electrophoresis can be used to separate repetitive DNA sequences. Gel electrophoresis separates DNA fragments because ______.

Accepted Answer

A)  double stranded moves slower than single stranded DNA 
B)  of ratios of guanine to cytosine 
C)  the DNA fragments have different lengths 
D)  of the salt concentration in the gel matrix 
A)  double stranded moves slower than single stranded DNA 
B)  of ratios of guanine to cytosine 
C)  the DNA fragments have different lengths 
D)  of the salt concentration in the gel matrix

Question 12

Nucleic acid probes are used to ______.

Accepted Answer

A)  find a specific nucleotide sequence 
B)  isolate bacterial genes 
C)  destroy clones that do not carry the recombinant plasmid of interest 
D)  synthesize a DNA strand complementary to a sticky end 
A)  find a specific nucleotide sequence 
B)  isolate bacterial genes 
C)  destroy clones that do not carry the recombinant plasmid of interest 
D)  synthesize a DNA strand complementary to a sticky end

Question 13

Of these steps,which occurs first in the production of a recombinant plasmid?

Accepted Answer

A)  cloning 
B)  construction of a genomic library 
C)  isolation of a plasmid from a bacterium 
D)  recombination 
A)  cloning 
B)  construction of a genomic library 
C)  isolation of a plasmid from a bacterium 
D)  recombination

Question 14

Please read the following scenario then answer the following question(s).
Molecular biologists have perfected DNA fingerprinting so that it is possible to use the technique to provide evidence to solve crimes and even identify a child's parents. Recently, a U.S. immigrant asked the U.S. Citizenship and Immigration Services for permission to have her young daughter who was living with grandparents in their homeland join her.Her request was denied because there was an apparent mix-up with the child's birth certificate and it could not be used as proof of maternity. Proof is required in cases such as this. The mother requested DNA fingerprinting to make her case. Samples of DNA were taken from the mother (Mom) and daughter (D1) as well as from another daughter (D2) and a son (S) living in the United States with her. Tandem repeat analysis was run on the four samples, and the results are shown here.
  
-You can use this DNA fingerprinting information to make a judgment about maternity because ______.

Accepted Answer

A)  the shade of DNA bands will differ 
B)  both mother and daughter contain only X chromosomes 
C)  of the position of the bands in the gel 
D)  of the size of the bands 
A)  the shade of DNA bands will differ 
B)  both mother and daughter contain only X chromosomes 
C)  of the position of the bands in the gel 
D)  of the size of the bands

Question 15

Restriction enzymes are obtained from ______.

Accepted Answer

A)  archaea 
B)  eukaryotes 
C)  viruses 
D)  bacteria 
A)  archaea 
B)  eukaryotes 
C)  viruses 
D)  bacteria

Question 16

To make restriction fragments,a DNA sample is treated with ______.

Accepted Answer

A)  DNA ligase 
B)  restriction enzymes 
C)  gel electrophoresis 
D)  PCR 
A)  DNA ligase 
B)  restriction enzymes 
C)  gel electrophoresis 
D)  PCR

Question 17

What technique is most commonly used to sequence entire genomes?

Accepted Answer

A)  the whole-genome shotgun method 
B)  the nucleic acid probe method 
C)  the plasmid method 
D)  the genetic marker method 
A)  the whole-genome shotgun method 
B)  the nucleic acid probe method 
C)  the plasmid method 
D)  the genetic marker method

Question 18

Which of these statements can be logically inferred from the amount of DNA shared by chimpanzees and humans?

Accepted Answer

A)  Humans and chimpanzees share a relatively recent common ancestor. 
B)  Humans evolved from chimpanzees. 
C)  Humans are unique and different from all other life forms. 
D)  Humans are a more complex life form than chimpanzees. 
A)  Humans and chimpanzees share a relatively recent common ancestor. 
B)  Humans evolved from chimpanzees. 
C)  Humans are unique and different from all other life forms. 
D)  Humans are a more complex life form than chimpanzees.

Question 19

Which of these can act as a vector to introduce new genes into a cell?

Accepted Answer

A)  humulin 
B)  GM 
C)  PCR 
D)  plasmids 
A)  humulin 
B)  GM 
C)  PCR 
D)  plasmids

Question 20

A DNA fragment with a sticky end that reads -ATTCG will bind with another DNA fragment with a sticky end that reads ______.

Accepted Answer

A)  TAAGC- 
B)  CGGAT- 
C)  GCCTA- 
D)  ATTGC- 
A)  TAAGC- 
B)  CGGAT- 
C)  GCCTA- 
D)  ATTGC-

Exam 12: DNA Technology

The world's first genetically engineered pharmaceutical product was ______.

The study of the full protein sets that genomes encode is _____.

"Sticky ends" are ______.

Gel electrophoresis separates DNA fragments on the basis of differences in their ______.

Of these steps,which one occurs earliest in the process of producing recombinant DNA?

Of the following,which is the last step in the production of a recombinant DNA plasmid?

Which of these is a genetically modified organism?

Cutting DNA with a particular restriction enzyme produces DNA fragments that can be separated by ______.

The scientific field that studies complete sets of genes is called ______.

The following figure shows that gel electrophoresis can be used to separate repetitive DNA sequences. Gel electrophoresis separates DNA fragments because ______.

Nucleic acid probes are used to ______.

Of these steps,which occurs first in the production of a recombinant plasmid?

Restriction enzymes are obtained from ______.

To make restriction fragments,a DNA sample is treated with ______.

What technique is most commonly used to sequence entire genomes?

Which of these statements can be logically inferred from the amount of DNA shared by chimpanzees and humans?

Which of these can act as a vector to introduce new genes into a cell?

A DNA fragment with a sticky end that reads -ATTCG will bind with another DNA fragment with a sticky end that reads ______.

Introduction- Biology Today

Essential Chemistry for Biology

The Molecules of Life

A Tour of the Cell

The Working Cell

Cellular Respiration: Obtaining Energy from Food

Photosynthesis: Using Light to Make Food

Cellular Reproduction: Cells from Cells

Patterns of Inheritance

The Structure and Function of DNA

How Genes Are Controlled

How Populations Evolve

How Biological Diversity Evolves

The Evolution of Microbial Life

Plants, Fungi, and the Move onto Land

The Evolution of Animals

An Introduction to Ecology and the Biosphere

Population Ecology

Communities and Ecosystems

Unifying Concepts of Animal Structure and Function

Nutrition and Digestion

Circulation and Respiration

The Body's Defenses

Hormones

Reproduction and Development

Nervous, Sensory, and Locomotor Systems

The Life of a Flowering Plant

The Working Plant

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