Question 1

Which among the following statements is not true about penetration?

Accepted Answer

A) The rate of penetration is always affected by heat. 
B) The penetration of fixatives can be improved by opening up a tissue sample. 
C) The concentration of the fixative always affects penetration. 
D) The presence of intestinal contents can inhibit the penetration of fixatives. 
A) The rate of penetration is always affected by heat. 
B) The penetration of fixatives can be improved by opening up a tissue sample. 
C) The concentration of the fixative always affects penetration. 
D) The presence of intestinal contents can inhibit the penetration of fixatives.

Question 2

Which among the following is a physical method of fixation?

Accepted Answer

A) Microwave fixation 
B) Cryo-preservation 
C) Immersion fixation 
D) Both A and B 
A) Microwave fixation 
B) Cryo-preservation 
C) Immersion fixation 
D) Both A and B

Question 3

Which of the following statements does not accurately describe formaldehyde?

Accepted Answer

A) It penetrates the tissues rapidly 
B) Formaldehyde fixes tissue specimens at a rapid rate of about 1mm per hour. 
C) It is an ideal fixative for nucleic acids 
D) It is one of the most commonly used aldehyde fixative 
A) It penetrates the tissues rapidly 
B) Formaldehyde fixes tissue specimens at a rapid rate of about 1mm per hour. 
C) It is an ideal fixative for nucleic acids 
D) It is one of the most commonly used aldehyde fixative

Question 4

What substance is added to formaldehyde in order to remove the precipitate produced by prolonged storage?

Accepted Answer

A) Acetone 
B) Acetic acid 
C) Methanol 
D) Distilled water 
A) Acetone 
B) Acetic acid 
C) Methanol 
D) Distilled water

Question 5

What is the suitable pH for fixation?

Accepted Answer

A) pH 2 to 3 
B) pH 4 to 6 
C) pH 6 to 8 
D) pH 7 to 9 
A) pH 2 to 3 
B) pH 4 to 6 
C) pH 6 to 8 
D) pH 7 to 9

Question 6

It is an excellent fixative that is chiefly recommended for tumor biopsies especially of the skin.

Accepted Answer

A) Heidenhain's Susa Solution 
B) Helly solution 
C) B-5 Fixative 
D) Zenker's Fluid 
A) Heidenhain's Susa Solution 
B) Helly solution 
C) B-5 Fixative 
D) Zenker's Fluid

Question 7

What is this type of metallic fixative that produces black precipitates?

Accepted Answer

A) Mercuric chloride 
B) Chromate fixative 
C) Lead fixative 
D) Heidenhain's Susa 
A) Mercuric chloride 
B) Chromate fixative 
C) Lead fixative 
D) Heidenhain's Susa

Question 8

All of the following choices below are fixatives of choice for electron microscopy, except:

Accepted Answer

A) 100% Methanol 
B) Osmium Tetroxide 
C) Glutaraldehyde 
D) Paraformaldehyde 
A) 100% Methanol 
B) Osmium Tetroxide 
C) Glutaraldehyde 
D) Paraformaldehyde

Question 9

Which of the following is not an example of a nuclear fixative?

Accepted Answer

A) Flemming's fluid 
B) Newcomer's fluid 
C) Heidenhain's Susa 
D) Orth's fluid 
A) Flemming's fluid 
B) Newcomer's fluid 
C) Heidenhain's Susa 
D) Orth's fluid

Question 10

As a histotechnician, one must know the basics of fixation before it occurs. One of the basic principles to remember is the ideal size of the tissue. Therefore, what is the ideal size of the tissue before fixing it?

Accepted Answer

A) 0)5 cm2 
B) 1 cm2 
C) 2 cm2 
D) 3 cm2 
A) 0)5 cm2 
B) 1 cm2 
C) 2 cm2 
D) 3 cm2

Question 11

This type of picric acid fixative is recommended for the preservation of embryos and pituitary biopsies.

Accepted Answer

A) Bouin's solution 
B) Brasil's Alcoholic picroformol 
C) Glacial acetic acid 
D) Carnoy's fluid 
A) Bouin's solution 
B) Brasil's Alcoholic picroformol 
C) Glacial acetic acid 
D) Carnoy's fluid

Question 12

This fixative is preferred for the preservation of chromosomes, lymph glands and urgent biopsies, as well as to preserve brain tissue for the identification of rabies.

Accepted Answer

A) Carnoy's Fluid 
B) 100% Methyl alcohol 
C) Bouin's Solution 
D) Muller's Fluid 
A) Carnoy's Fluid 
B) 100% Methyl alcohol 
C) Bouin's Solution 
D) Muller's Fluid

Question 13

It is the fixative of choice used to preserve touch preparations and for staining procedures of Wright- Giemsa.

Accepted Answer

A) Acetone 
B) Formalin 
C) Isopropyl alcohol 
D) Osmic acid 
A) Acetone 
B) Formalin 
C) Isopropyl alcohol 
D) Osmic acid

Question 14

What are the characteristics of a good fixative?

Accepted Answer

A) It must inhibit bacterial decomposition and autolysis. 
B) It must permit rapid and even penetration of tissues. 
C) Both A and B 
D) None of the above 
A) It must inhibit bacterial decomposition and autolysis. 
B) It must permit rapid and even penetration of tissues. 
C) Both A and B 
D) None of the above

Question 15

Heat fixation should be used on the following except for:

Accepted Answer

A) Gram stain 
B) Capsular staining 
C) Endospore staining 
D) Acid Fast stain 
A) Gram stain 
B) Capsular staining 
C) Endospore staining 
D) Acid Fast stain

Question 16

One disadvantage of using perfusion as a fixation process is:

Accepted Answer

A) It is a costly process 
B) It makes the subject paralyzed 
C) The volume of fixative used is smaller 
D) The tissue does not die until it is fixed 
A) It is a costly process 
B) It makes the subject paralyzed 
C) The volume of fixative used is smaller 
D) The tissue does not die until it is fixed

Question 17

Which of the following is used as dehydrating agent and as a fixative at the same time?

Accepted Answer

A) Acetone 
B) Mercuric Chloride 
C) Acetic acid 
D) Both A and B 
A) Acetone 
B) Mercuric Chloride 
C) Acetic acid 
D) Both A and B

Question 18

Among the choices below, which of the following fixatives is preferred for the preservation of mucopolysaccharides and nuclear proteins?

Accepted Answer

A) Carnoy's Fluid 
B) 100% Methyl Alcohol 
C) Newcomer's Fluid 
D) Bouin's Solution 
A) Carnoy's Fluid 
B) 100% Methyl Alcohol 
C) Newcomer's Fluid 
D) Bouin's Solution

Question 19

What is the usual fixation time when using 10% formaldehyde?

Accepted Answer

A) 3 hours 
B) 12 hours 
C) 24 hours 
D) 72 hours 
A) 3 hours 
B) 12 hours 
C) 24 hours 
D) 72 hours

Question 20

A histotechnician receives a sample of bone marrow for biopsy. What fixative of choice is suitable for bone marrow preservation?

Accepted Answer

A) Alcohol 
B) B-5 fixative 
C) Carnoy's fluid 
D) Orth's fluid 
A) Alcohol 
B) B-5 fixative 
C) Carnoy's fluid 
D) Orth's fluid

Which among the following statements is not true about penetration?

Which among the following is a physical method of fixation?

Which of the following statements does not accurately describe formaldehyde?

What substance is added to formaldehyde in order to remove the precipitate produced by prolonged storage?

What is the suitable pH for fixation?

It is an excellent fixative that is chiefly recommended for tumor biopsies especially of the skin.

What is this type of metallic fixative that produces black precipitates?

All of the following choices below are fixatives of choice for electron microscopy, except:

Which of the following is not an example of a nuclear fixative?

As a histotechnician, one must know the basics of fixation before it occurs. One of the basic principles to remember is the ideal size of the tissue. Therefore, what is the ideal size of the tissue before fixing it?

This type of picric acid fixative is recommended for the preservation of embryos and pituitary biopsies.

This fixative is preferred for the preservation of chromosomes, lymph glands and urgent biopsies, as well as to preserve brain tissue for the identification of rabies.

It is the fixative of choice used to preserve touch preparations and for staining procedures of Wright- Giemsa.

What are the characteristics of a good fixative?

Heat fixation should be used on the following except for:

One disadvantage of using perfusion as a fixation process is:

Which of the following is used as dehydrating agent and as a fixative at the same time?

Among the choices below, which of the following fixatives is preferred for the preservation of mucopolysaccharides and nuclear proteins?

What is the usual fixation time when using 10% formaldehyde?

A histotechnician receives a sample of bone marrow for biopsy. What fixative of choice is suitable for bone marrow preservation?

Laboratory Operations

Microtomy

Processing and Embedding

Staining

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Exam 1: Fixation

Which among the following statements is not true about penetration?

Which among the following is a physical method of fixation?

Which of the following statements does not accurately describe formaldehyde?

What substance is added to formaldehyde in order to remove the precipitate produced by prolonged storage?

What is the suitable pH for fixation?

It is an excellent fixative that is chiefly recommended for tumor biopsies especially of the skin.

What is this type of metallic fixative that produces black precipitates?

All of the following choices below are fixatives of choice for electron microscopy, except:

Which of the following is not an example of a nuclear fixative?

As a histotechnician, one must know the basics of fixation before it occurs. One of the basic principles to remember is the ideal size of the tissue. Therefore, what is the ideal size of the tissue before fixing it?

This type of picric acid fixative is recommended for the preservation of embryos and pituitary biopsies.

This fixative is preferred for the preservation of chromosomes, lymph glands and urgent biopsies, as well as to preserve brain tissue for the identification of rabies.

It is the fixative of choice used to preserve touch preparations and for staining procedures of Wright- Giemsa.

What are the characteristics of a good fixative?

Heat fixation should be used on the following except for:

One disadvantage of using perfusion as a fixation process is:

Which of the following is used as dehydrating agent and as a fixative at the same time?

Among the choices below, which of the following fixatives is preferred for the preservation of mucopolysaccharides and nuclear proteins?

What is the usual fixation time when using 10% formaldehyde?

A histotechnician receives a sample of bone marrow for biopsy. What fixative of choice is suitable for bone marrow preservation?

Laboratory Operations

Microtomy

Processing and Embedding

Staining

Filters