Question 1

It is used in fixing brain tissues for the diagnosis of rabies.

Accepted Answer

A) Heat fixation 
B) Alcohol 
C) Acetone 
D) Osmic acid 
A) Heat fixation 
B) Alcohol 
C) Acetone 
D) Osmic acid

Question 2

If a histotechnician receives a sample that contains glycogen, what fixative of choice is suitable for the demonstration and preservation of glycogen?

Accepted Answer

A) Neutral buffered formol saline 
B) Rossman's fluid 
C) Carnoy's fluid 
D) Heidenhain's fluid 
A) Neutral buffered formol saline 
B) Rossman's fluid 
C) Carnoy's fluid 
D) Heidenhain's fluid

Question 3

What is the ideal preparation or concentration of formaldehyde?

Accepted Answer

A) 15% solution 
B) 3% solution 
C) 8% solution 
D) 10% solution 
A) 15% solution 
B) 3% solution 
C) 8% solution 
D) 10% solution

Question 4

What are the advantages of utilizing frozen sections?

Accepted Answer

A) Lack of morphological detail 
B) Much faster than other forms of fixation 
C) Not exposed to organic solvents 
D) Both B and C 
A) Lack of morphological detail 
B) Much faster than other forms of fixation 
C) Not exposed to organic solvents 
D) Both B and C

Question 5

Which of the following best describes fixation?

Accepted Answer

A) A procedure adopted to kill, harden and preserve the tissue for microscopic study by the use of substances like xylene. 
B) A procedure adopted to kill, harden and preserve the tissue for microscopic study by the use of substances like mercuric chloride. 
C) The procedure in which the ionic materials are removed from the tissue. 
D) The process of removing intracellular and extracellular H2O. 
A) A procedure adopted to kill, harden and preserve the tissue for microscopic study by the use of substances like xylene. 
B) A procedure adopted to kill, harden and preserve the tissue for microscopic study by the use of substances like mercuric chloride. 
C) The procedure in which the ionic materials are removed from the tissue. 
D) The process of removing intracellular and extracellular H2O.

Question 6

This type of fixative is ideal for the diagnosis of water diffusible enzymes especially phosphatases and lipase but produces unavoidable shrinkage and deformation.

Accepted Answer

A) Alcohol 
B) Acetone 
C) Heat 
D) Acid 
A) Alcohol 
B) Acetone 
C) Heat 
D) Acid

Question 7

Which of the following is the fixative of choice for fixing brain tissue?

Accepted Answer

A) Acetone 
B) Alcohol 
C) Carnoy's Fluid 
D) Both A and C 
A) Acetone 
B) Alcohol 
C) Carnoy's Fluid 
D) Both A and C

Question 8

It is a type of fixative that is ideal for small tissue fragments and must be used in higher concentrations to better fix the sample. What is this type of fixative?

Accepted Answer

A) Alcohol 
B) Acids 
C) Aldehydes 
D) Metallic fixatives 
A) Alcohol 
B) Acids 
C) Aldehydes 
D) Metallic fixatives

Question 9

John Daniel knows that concentrated solutions of formaldehyde must never be neutralized as this will:

Accepted Answer

A) Cause increased bleaching of the tissues 
B) Increase the rate of decomposition of formaldehyde 
C) Stimulate violent chemical explosions 
D) Cause formalin to become ineffective as a fixative 
A) Cause increased bleaching of the tissues 
B) Increase the rate of decomposition of formaldehyde 
C) Stimulate violent chemical explosions 
D) Cause formalin to become ineffective as a fixative

Question 10

It is the fixative of choice to better exhibit Rickettsiae and other bacteria.

Accepted Answer

A) Muller's Fluid 
B) Orth's Fluid 
C) Heidenhain's Susa 
D) Zenker-formol 
A) Muller's Fluid 
B) Orth's Fluid 
C) Heidenhain's Susa 
D) Zenker-formol

Question 11

Which among the following is not a property of acetic acid?

Accepted Answer

A) It causes the shrinkage of collagen 
B) It is an agent used for denaturation 
C) It does not generally fix proteins 
D) It is a precipitating fixative 
A) It causes the shrinkage of collagen 
B) It is an agent used for denaturation 
C) It does not generally fix proteins 
D) It is a precipitating fixative

Question 12

Which of the following properties of Mercuric chloride makes it restricted for use?

Accepted Answer

A) Toxic 
B) Corrosive 
C) Dangerous to the environment 
D) All of the above 
A) Toxic 
B) Corrosive 
C) Dangerous to the environment 
D) All of the above

Question 13

This process of fixation preserves structures in a state that is chemically and structurally as close to the living tissue as possible.

Accepted Answer

A) Heat fixation 
B) Chemical fixation 
C) Perfusion 
D) Immersion 
A) Heat fixation 
B) Chemical fixation 
C) Perfusion 
D) Immersion

Question 14

Which of the following tissue samples is fixed first before sectioning or grossing?

Accepted Answer

A) Heart 
B) Brain 
C) Liver 
D) Pancreas 
A) Heart 
B) Brain 
C) Liver 
D) Pancreas

Question 15

Allan understands that this metallic fixative has been discovered to react with viruses.

Accepted Answer

A) Potassium dichromate 
B) Osmium tetroxide 
C) Chromic acid 
D) Mercuric chloride 
A) Potassium dichromate 
B) Osmium tetroxide 
C) Chromic acid 
D) Mercuric chloride

Question 16

On which of the following specimens do you primarily use Sudan stains?

Accepted Answer

A) nerve tissue 
B) fat 
C) muscle fibers 
D) bone 
A) nerve tissue 
B) fat 
C) muscle fibers 
D) bone

Question 17

What is the formula needed to prepare a 10% formol-saline?

Accepted Answer

A) 100ml of 40% formaldehyde, 9g of NaCl, and 900ml of distilled water 
B) 100ml of 10% formaldehyde, 10g of NaCl, and 1000ml of distilled water 
C) 100ml of 5% formaldehyde, 11g of NaCl, and 1200ml of distilled water 
D) 100ml of 20% formaldehyde, 5g of NaCl, and 1500ml of distilled water 
A) 100ml of 40% formaldehyde, 9g of NaCl, and 900ml of distilled water 
B) 100ml of 10% formaldehyde, 10g of NaCl, and 1000ml of distilled water 
C) 100ml of 5% formaldehyde, 11g of NaCl, and 1200ml of distilled water 
D) 100ml of 20% formaldehyde, 5g of NaCl, and 1500ml of distilled water

Question 18

This type of fixative is preferred for the diagnosis of early degenerative processes and tissue necrosis.

Accepted Answer

A) Muller's Fluid 
B) Orth's Fluid 
C) Heidenhain's Susa 
D) Zenker-formol 
A) Muller's Fluid 
B) Orth's Fluid 
C) Heidenhain's Susa 
D) Zenker-formol

Question 19

The following are the preferred fixatives to be used on lipids except:

Accepted Answer

A) Frozen sections 
B) Glutaraldehyde 
C) Neutral buffered formalin 
D) Osmium tetroxide 
A) Frozen sections 
B) Glutaraldehyde 
C) Neutral buffered formalin 
D) Osmium tetroxide

Question 20

Which of the following retards fixation of tissues?

Accepted Answer

A) Smaller and thinner tissues 
B) Agitation 
C) Moderate heat at about 37-56 degree Celsius. 
D) Presence of fat 
A) Smaller and thinner tissues 
B) Agitation 
C) Moderate heat at about 37-56 degree Celsius. 
D) Presence of fat

It is used in fixing brain tissues for the diagnosis of rabies.

If a histotechnician receives a sample that contains glycogen, what fixative of choice is suitable for the demonstration and preservation of glycogen?

What is the ideal preparation or concentration of formaldehyde?

What are the advantages of utilizing frozen sections?

Which of the following best describes fixation?

This type of fixative is ideal for the diagnosis of water diffusible enzymes especially phosphatases and lipase but produces unavoidable shrinkage and deformation.

Which of the following is the fixative of choice for fixing brain tissue?

It is a type of fixative that is ideal for small tissue fragments and must be used in higher concentrations to better fix the sample. What is this type of fixative?

John Daniel knows that concentrated solutions of formaldehyde must never be neutralized as this will:

It is the fixative of choice to better exhibit Rickettsiae and other bacteria.

Which among the following is not a property of acetic acid?

Which of the following properties of Mercuric chloride makes it restricted for use?

This process of fixation preserves structures in a state that is chemically and structurally as close to the living tissue as possible.

Which of the following tissue samples is fixed first before sectioning or grossing?

Allan understands that this metallic fixative has been discovered to react with viruses.

On which of the following specimens do you primarily use Sudan stains?

What is the formula needed to prepare a 10% formol-saline?

This type of fixative is preferred for the diagnosis of early degenerative processes and tissue necrosis.

The following are the preferred fixatives to be used on lipids except:

Which of the following retards fixation of tissues?

Laboratory Operations

Microtomy

Processing and Embedding

Staining

Filters

Exam 1: Fixation

It is used in fixing brain tissues for the diagnosis of rabies.

If a histotechnician receives a sample that contains glycogen, what fixative of choice is suitable for the demonstration and preservation of glycogen?

What is the ideal preparation or concentration of formaldehyde?

What are the advantages of utilizing frozen sections?

Which of the following best describes fixation?

This type of fixative is ideal for the diagnosis of water diffusible enzymes especially phosphatases and lipase but produces unavoidable shrinkage and deformation.

Which of the following is the fixative of choice for fixing brain tissue?

It is a type of fixative that is ideal for small tissue fragments and must be used in higher concentrations to better fix the sample. What is this type of fixative?

John Daniel knows that concentrated solutions of formaldehyde must never be neutralized as this will:

It is the fixative of choice to better exhibit Rickettsiae and other bacteria.

Which among the following is not a property of acetic acid?

Which of the following properties of Mercuric chloride makes it restricted for use?

This process of fixation preserves structures in a state that is chemically and structurally as close to the living tissue as possible.

Which of the following tissue samples is fixed first before sectioning or grossing?

Allan understands that this metallic fixative has been discovered to react with viruses.

On which of the following specimens do you primarily use Sudan stains?

What is the formula needed to prepare a 10% formol-saline?

This type of fixative is preferred for the diagnosis of early degenerative processes and tissue necrosis.

The following are the preferred fixatives to be used on lipids except:

Which of the following retards fixation of tissues?

Laboratory Operations

Microtomy

Processing and Embedding

Staining

Filters