Question 1

Which of the following is not part of a rotary microtome?

Accepted Answer

A) Flywheel 
B) Pawl 
C) Base 
D) Stage 
A) Flywheel 
B) Pawl 
C) Base 
D) Stage

Question 2

This involves the removal of gross nicks on the knife edge to remove blemishes and grinding the cutting edge of the knife on a stone to acquire an even edge. What do you call this procedure?

Accepted Answer

A) Stropping 
B) Honing 
C) Sharpening 
D) Cutting 
A) Stropping 
B) Honing 
C) Sharpening 
D) Cutting

Question 3

What do you call the process whereby tissues are cut into uniformly thin slices or sections with the aid of a machine?

Accepted Answer

A) Cutting 
B) Sectioning 
C) Honing 
D) Stropping 
A) Cutting 
B) Sectioning 
C) Honing 
D) Stropping

Question 4

This type of knife measures usually about 120 mm in length with both sides concave and it is recommended for cutting paraffin embedded sections on a rotary microtome.

Accepted Answer

A) Biconcave knife 
B) Plane-Concave knife 
C) Plane-wedge knife 
D) None of the above 
A) Biconcave knife 
B) Plane-Concave knife 
C) Plane-wedge knife 
D) None of the above

Question 5

All of the following statements are true regarding the care of a microtome, except:

Accepted Answer

A) After sectioning, all the accumulated paraffin and small pieces of tissues must be brushed away with steel brush. 
B) After carefully drying the machine and knife holder, the parts should be wiped with xylol. 
C) Movable portions should be oiled thoroughly to prevent rusting. 
D) Cover the microtome when not in use. 
A) After sectioning, all the accumulated paraffin and small pieces of tissues must be brushed away with steel brush. 
B) After carefully drying the machine and knife holder, the parts should be wiped with xylol. 
C) Movable portions should be oiled thoroughly to prevent rusting. 
D) Cover the microtome when not in use.

Question 6

In Celloidin sections, what is the usual thickness range of the cuts made?

Accepted Answer

A) 10 to 15 microns 
B) 5 to 10 microns 
C) 3 to 5 microns 
D) 1 to 3 microns 
A) 10 to 15 microns 
B) 5 to 10 microns 
C) 3 to 5 microns 
D) 1 to 3 microns

Question 7

During section cutting, the sections fail to form ribbons due to the surfaces and edges of the block that are not parallel. What could be the best possible remedy for this?

Accepted Answer

A) Repeat impregnation 
B) Repeat paraffin impregnation, and re-embed 
C) Re-trim the block 
D) Repeat the whole procedure 
A) Repeat impregnation 
B) Repeat paraffin impregnation, and re-embed 
C) Re-trim the block 
D) Repeat the whole procedure

Question 8

All of the following descriptions are true about Cryostat or the Cold Microtome, except:

Accepted Answer

A) Cryostat is an apparatus used in fresh tissue microtomy. 
B) It is primarily used for cutting tissue sections at 0.5 micra. 
C) It is capable of freezing the tissue into the block holder to the correct degree of hardness to facilitate easier and faster sectioning. 
D) It permits rapid preparation of tissue biopsies for surgical Pathology. 
A) Cryostat is an apparatus used in fresh tissue microtomy. 
B) It is primarily used for cutting tissue sections at 0.5 micra. 
C) It is capable of freezing the tissue into the block holder to the correct degree of hardness to facilitate easier and faster sectioning. 
D) It permits rapid preparation of tissue biopsies for surgical Pathology.

Question 9

In a Cold microtome, the needed temperature that must be maintained is between:

Accepted Answer

A) -5 to -15 degrees Celsius 
B) -35 t0 -45 degrees Celsius 
C) -5 to -30 degrees Celsius 
D) -1 to -10 degrees Celsius 
A) -5 to -15 degrees Celsius 
B) -35 t0 -45 degrees Celsius 
C) -5 to -30 degrees Celsius 
D) -1 to -10 degrees Celsius

Question 10

This type of hone is much coarser and is used only for badly nicked knives?

Accepted Answer

A) Belgium Yellow stone 
B) Arkansas stone 
C) Fine Carborundum stone 
D) Either B or C 
A) Belgium Yellow stone 
B) Arkansas stone 
C) Fine Carborundum stone 
D) Either B or C

Which of the following is not part of a rotary microtome?

This involves the removal of gross nicks on the knife edge to remove blemishes and grinding the cutting edge of the knife on a stone to acquire an even edge. What do you call this procedure?

What do you call the process whereby tissues are cut into uniformly thin slices or sections with the aid of a machine?

This type of knife measures usually about 120 mm in length with both sides concave and it is recommended for cutting paraffin embedded sections on a rotary microtome.

All of the following statements are true regarding the care of a microtome, except:

In Celloidin sections, what is the usual thickness range of the cuts made?

During section cutting, the sections fail to form ribbons due to the surfaces and edges of the block that are not parallel. What could be the best possible remedy for this?

All of the following descriptions are true about Cryostat or the Cold Microtome, except:

In a Cold microtome, the needed temperature that must be maintained is between:

This type of hone is much coarser and is used only for badly nicked knives?

Fixation

Laboratory Operations

Processing and Embedding

Staining

Filters

Exam 3: Microtomy

Which of the following is not part of a rotary microtome?

This involves the removal of gross nicks on the knife edge to remove blemishes and grinding the cutting edge of the knife on a stone to acquire an even edge. What do you call this procedure?

What do you call the process whereby tissues are cut into uniformly thin slices or sections with the aid of a machine?

This type of knife measures usually about 120 mm in length with both sides concave and it is recommended for cutting paraffin embedded sections on a rotary microtome.

All of the following statements are true regarding the care of a microtome, except:

In Celloidin sections, what is the usual thickness range of the cuts made?

During section cutting, the sections fail to form ribbons due to the surfaces and edges of the block that are not parallel. What could be the best possible remedy for this?

All of the following descriptions are true about Cryostat or the Cold Microtome, except:

In a Cold microtome, the needed temperature that must be maintained is between:

This type of hone is much coarser and is used only for badly nicked knives?

Fixation

Laboratory Operations

Processing and Embedding

Staining

Filters