Question 1

All of the following are common extrapulmonary infection sites for tuberculosis except:

Accepted Answer

A) kidneys. 
B) gastrointestinal. 
C) liver. 
D) spleen. 
A) kidneys. 
B) gastrointestinal. 
C) liver. 
D) spleen. B

Question 2

All of these stains are commonly used to visualize mycobacteria on a smear except:

Accepted Answer

A) Ziehl-Neelsen. 
B) Kinyoun. 
C) auramine. 
D) acridine orange. 
A) Ziehl-Neelsen. 
B) Kinyoun. 
C) auramine. 
D) acridine orange. D

Question 3

Decontaminating agents used in the decontamination-digestion process include all of the following except:

Accepted Answer

A) sodium hydroxide. 
B) N-acetyl-1-cysteine. 
C) benzalkonium chloride. 
D) hydrochloric acid. 
A) sodium hydroxide. 
B) N-acetyl-1-cysteine. 
C) benzalkonium chloride. 
D) hydrochloric acid. D

Question 4

How long does it take to detect Mycobacterium spp.with the BACTEC method?

Accepted Answer

A) Less than 2 weeks 
B) 3 weeks 
C) 4 weeks 
D) 6 weeks 
A) Less than 2 weeks 
B) 3 weeks 
C) 4 weeks 
D) 6 weeks

Question 5

A patient with Hodgkin's disease is feeling bad, so he pays a visit to his physician.The physician's clinical examination reveals submandibular lymphadenitis, subcutaneous nodules, painful swellings, ulcers progressing to abscesses, and draining fistulas.What organism is probably producing these symptoms?

Accepted Answer

A) Mycobacterium tuberculosis 
B) M.avium 
C) M.gordonae 
D) M.haemophilum 
A) Mycobacterium tuberculosis 
B) M.avium 
C) M.gordonae 
D) M.haemophilum

Question 6

What are nonchromogens?

Accepted Answer

A) Species that produce carotene pigment upon exposure to light 
B) Species that produce pigment in the light or the dark 
C) Species whose colonies remain buff colored after exposure to light 
D) Species whose colonies fluoresce under ultraviolet light 
A) Species that produce carotene pigment upon exposure to light 
B) Species that produce pigment in the light or the dark 
C) Species whose colonies remain buff colored after exposure to light 
D) Species whose colonies fluoresce under ultraviolet light

Question 7

If a person has had tuberculosis during his or her lifetime, how likely is it that the person will get the disease again?

Accepted Answer

A) 5% to 15% 
B) 20% to 25% 
C) 1% 
D) 50% 
A) 5% to 15% 
B) 20% to 25% 
C) 1% 
D) 50%

Question 8

Middlebrook 7H10 and 7H11 media are enriched with all of the following except:

Accepted Answer

A) oleic acid. 
B) bovine albumin. 
C) heme. 
D) beef catalase. 
A) oleic acid. 
B) bovine albumin. 
C) heme. 
D) beef catalase.

Question 9

What is the purpose of the digestion-decontamination processing of specimens submitted for mycobacterial culture?

Accepted Answer

A) To release all bacteria in the sample 
B) To make the specimen easier to plate 
C) To allow chemical decontaminants to kill nonmycobacterial organisms 
D) To liquefy the outer cell wall of the mycobacteria so that they will grow faster 
A) To release all bacteria in the sample 
B) To make the specimen easier to plate 
C) To allow chemical decontaminants to kill nonmycobacterial organisms 
D) To liquefy the outer cell wall of the mycobacteria so that they will grow faster

Question 10

What are scotochromogens?

Accepted Answer

A) Species that produce carotene pigment upon exposure to light 
B) Species that produce pigment in the light or the dark 
C) Species whose colonies remain buff colored after exposure to light 
D) Species whose colonies fluoresce under ultraviolet light 
A) Species that produce carotene pigment upon exposure to light 
B) Species that produce pigment in the light or the dark 
C) Species whose colonies remain buff colored after exposure to light 
D) Species whose colonies fluoresce under ultraviolet light

Question 11

A 35-year-old man travels on a multidestination vacation over a 2 month period, including lengthy stops in tropical Africa and Southeast Asia.Six weeks after returning come home to the United States, he develops a productive cough, fatigue, weight loss, low-grade fever, and night sweats.What disease should the physician consider as a result of his travel history?

Accepted Answer

A) Streptococcal pneumonia 
B) Primary atypical pneumonia 
C) Tuberculosis (T
B)  
D) Pneumonia caused by gram-negative rods 
A) Streptococcal pneumonia 
B) Primary atypical pneumonia 
C) Tuberculosis (T
B)  
D) Pneumonia caused by gram-negative rods

Question 12

What are photochromogens?

Accepted Answer

A) Species that produce carotene pigment upon exposure to light 
B) Species that produce pigment in the light or the dark 
C) Species whose colonies remain buff colored after exposure to light 
D) Species whose colonies fluoresce under ultraviolet light 
A) Species that produce carotene pigment upon exposure to light 
B) Species that produce pigment in the light or the dark 
C) Species whose colonies remain buff colored after exposure to light 
D) Species whose colonies fluoresce under ultraviolet light

Question 13

What is the antigen used in the purified protein derivative (PPD) skin test?

Accepted Answer

A) A purified protein from the nucleus of Mycobacterium tuberculosis 
B) A purified piece of DNA from M.tuberculosis 
C) A purified protein from the cell wall of M.tuberculosis 
D) A piece of ribosomal RNA from M.tuberculosis 
A) A purified protein from the nucleus of Mycobacterium tuberculosis 
B) A purified piece of DNA from M.tuberculosis 
C) A purified protein from the cell wall of M.tuberculosis 
D) A piece of ribosomal RNA from M.tuberculosis

Question 14

What is the optimum culture incubation time for most mycobacteria associated with human disease?

Accepted Answer

A) 2 to 6 weeks 
B) 1 to 2 weeks 
C) 5 to 7 days 
D) 3 to 5 days 
A) 2 to 6 weeks 
B) 1 to 2 weeks 
C) 5 to 7 days 
D) 3 to 5 days

Question 15

All of the following are biochemical tests for the identification of Mycobacterium spp.except:

Accepted Answer

A) gelatin liquefaction. 
B) hydrolysis of Tween 80. 
C) iron uptake. 
D) arylsulfatase. 
A) gelatin liquefaction. 
B) hydrolysis of Tween 80. 
C) iron uptake. 
D) arylsulfatase.

Question 16

When designing a mycobacterial laboratory, the designers should build in all the following engineering controls to keep the workers safe except:

Accepted Answer

A) inside air vents to outside. 
B) nonrecirculating ventilation system. 
C) negative air pressure. 
D) separate room from main laboratory. 
A) inside air vents to outside. 
B) nonrecirculating ventilation system. 
C) negative air pressure. 
D) separate room from main laboratory.

Question 17

What is the recommended contact time for most disinfectants in a mycobacteriology laboratory?

Accepted Answer

A) 5 minutes 
B) 10 to 30 minutes 
C) 10 minutes 
D) 60 minutes 
A) 5 minutes 
B) 10 to 30 minutes 
C) 10 minutes 
D) 60 minutes

Question 18

What component of mycobacteria is used for identification in nucleic acid hybridization assays?

Accepted Answer

A) DNA 
B) Ribosomal RNA 
C) Transfer RNA 
D) Messenger RNA 
A) DNA 
B) Ribosomal RNA 
C) Transfer RNA 
D) Messenger RNA

Question 19

What Mycobacterium spp.fails to grow in vitro?

Accepted Answer

A) Mycobacterium tuberculosis 
B) M.leprae 
C) M.avium 
D) M.marinum 
A) Mycobacterium tuberculosis 
B) M.leprae 
C) M.avium 
D) M.marinum

Question 20

What type of media is recommended for the recovery of Mycobacterium haemophilum?

Accepted Answer

A) Sheep blood agar (SB
A)  
B) Chocolate 
C) Polysaccharide egg antigen (PE
A)  
D) Löwenstein-Jensen (LJ) 
A) Sheep blood agar (SB
A)  
B) Chocolate 
C) Polysaccharide egg antigen (PE
A)  
D) Löwenstein-Jensen (LJ)

Exam 26: Mycobacterium Tuberculosis and Nontuberculous Mycobacteria

All of the following are common extrapulmonary infection sites for tuberculosis except:

All of these stains are commonly used to visualize mycobacteria on a smear except:

Decontaminating agents used in the decontamination-digestion process include all of the following except:

How long does it take to detect Mycobacterium spp.with the BACTEC method?

What are nonchromogens?

If a person has had tuberculosis during his or her lifetime, how likely is it that the person will get the disease again?

Middlebrook 7H10 and 7H11 media are enriched with all of the following except:

What is the purpose of the digestion-decontamination processing of specimens submitted for mycobacterial culture?

What are scotochromogens?

What are photochromogens?

What is the antigen used in the purified protein derivative (PPD) skin test?

What is the optimum culture incubation time for most mycobacteria associated with human disease?

All of the following are biochemical tests for the identification of Mycobacterium spp.except:

When designing a mycobacterial laboratory, the designers should build in all the following engineering controls to keep the workers safe except:

What is the recommended contact time for most disinfectants in a mycobacteriology laboratory?

What component of mycobacteria is used for identification in nucleic acid hybridization assays?

What Mycobacterium spp.fails to grow in vitro?

What type of media is recommended for the recovery of Mycobacterium haemophilum?

Bacterial Cell Structure, Physiology, Metabolism, and Genetics

Host-Parasite Interaction

The Laboratory Role in Infection Control

Control of Microorganisms

Performance Improvement in the Microbiology Laboratory

Specimen Collection and Processing

Microscopic Examination of Materials From Infected Sites

Use of Colony Morphology for the Presumptive Identification of Microorganisms

Biochemical Identification of Gram-Negative Bacteria

Immunodiagnosis of Infectious Diseases

Applications of Molecular Diagnostics

Antimicrobial Agent Mechanisms of Action and Resistance

Antimicrobial Susceptibility Testing

Staphylococci

Streptococcus, Enterococcus, and Other Catalase-Negative, Gram-Positive Cocci

Aerobic Gram-Positive Bacilli

Neisseria Species and Moraxella Catarrhalis

Haemophilus and Other Fastidious Gram-Negative Bacilli

Enterobacteriaceae

Vibrio, Aeromonas, Plesiomonas, and Campylobacter Species

Nonfermenting and Miscellaneous Gram-Negative Bacilli

Anaerobes of Clinical Importance

The Spirochetes

Chlamydia, Rickettsia and Similar Organisms

Mycoplasma and Ureaplasma

Medically Significant Fungi

Diagnostic Parasitology

Clinical Virology

Agents of Bioterror and Forensic Microbiology

Biofilms: Architects of Disease

Upper and Lower Respiratory Tract Infections

Skin and Soft Tissue Infections

Gastrointestinal Infections and Food Poisoning

Infections of the Central Nervous System

Bacteremia and Sepsis

Urinary Tract Infections

Genital Infections and Sexually Transmitted Diseases

Infections in Special Populations

Zoonotic Diseases

Ocular Infections

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