Question 1

In lieu of an immune response, plants use RNA silencing to defend against virus infection.

Accepted Answer

A) True 
 B)False  True

Question 2

The 5'-terminal 200nts on the cucumber mosaic virus genome function as a promoter for the synthesis of the minus-strand RNA.

Accepted Answer

A) True 
 B)False  False

Question 3

Which of the following describes the hypersensitive response in plants infected with the cucumber mosaic virus?

Accepted Answer

A)  The virus moves into the vascular tissue and causes a systemic infection. 
B)  The virus causes an asymptomatic infection. 
C)  Virus replication is limited to a local region of infection. 
D)  The plant is completely resistant to the virus. 
E)  Virus replication only occurs in the newest tissues on the plant. 
A)  The virus moves into the vascular tissue and causes a systemic infection. 
B)  The virus causes an asymptomatic infection. 
C)  Virus replication is limited to a local region of infection. 
D)  The plant is completely resistant to the virus. 
E)  Virus replication only occurs in the newest tissues on the plant. C

Question 4

All of the following describe a function of the movement protein from cucumber mosaic virus EXCEPT:

Accepted Answer

A)  It allows the viral RNA to move between host cells. 
B)  It interacts with the host cytoskeleton. 
C)  It dilates the plasmodesmata. 
D)  It binds to viral genomic RNAs. 
E)  It causes the viral RNA to be taken up by aphids. 
A)  It allows the viral RNA to move between host cells. 
B)  It interacts with the host cytoskeleton. 
C)  It dilates the plasmodesmata. 
D)  It binds to viral genomic RNAs. 
E)  It causes the viral RNA to be taken up by aphids.

Question 5

Which of the following statements about cucumber mosaic virus is FALSE?

Accepted Answer

A)  It has an enveloped helical nucleocapsid. 
B)  It has a wide range of host species that it can infect. 
C)  Its genome is composed of positive-strand RNA. 
D)  It is transmitted by aphids. 
E)  It can cause asymptomatic infections in some plants. 
A)  It has an enveloped helical nucleocapsid. 
B)  It has a wide range of host species that it can infect. 
C)  Its genome is composed of positive-strand RNA. 
D)  It is transmitted by aphids. 
E)  It can cause asymptomatic infections in some plants.

Question 6

Which of the following describes the first step in the replication cycle of the cucumber mosaic virus?

Accepted Answer

A)  Synthesis of minus-strand copies of the genome. 
B)  Synthesis of plus-strand copies of the genome. 
C)  Copying of viral genome into dsDNA. 
D)  Copying of the viral genome into mRNA. 
E)  Translation of the genome into protein. 
A)  Synthesis of minus-strand copies of the genome. 
B)  Synthesis of plus-strand copies of the genome. 
C)  Copying of viral genome into dsDNA. 
D)  Copying of the viral genome into mRNA. 
E)  Translation of the genome into protein.

Question 7

Which of the following proteins from cucumber mosaic virus acts at the RNA-dependent RNA polymerase?

Accepted Answer

A)  Protein 1a 
B)  Protein 2a 
C)  Protein 2b 
D)  Movement Protein 
E)  Coat Protein 
A)  Protein 1a 
B)  Protein 2a 
C)  Protein 2b 
D)  Movement Protein 
E)  Coat Protein

Question 8

What is the role of the plasmodesmata in the replication of cucumber mosaic virus?

Accepted Answer

A)  They allow release of progeny virions from host cells. 
B)  They are the sites of entry of capsids into the host cell. 
C)  They are the sites of viral replication inside of plant cells. 
D)  They allow movement of virus genomes between neighboring cells. 
E)  They are involved in assembly of progeny virions. 
A)  They allow release of progeny virions from host cells. 
B)  They are the sites of entry of capsids into the host cell. 
C)  They are the sites of viral replication inside of plant cells. 
D)  They allow movement of virus genomes between neighboring cells. 
E)  They are involved in assembly of progeny virions.

Question 9

Which of the following enzymes can transfer an amino acid to the 3' end of the genome of cucumber mosaic virus?

Accepted Answer

A)  Elongation factor Tu 
B)  Aminoacyl tRNA synthetase 
C)  Nucleotidyltransferase. 
D)  Ribosomal protein S1. 
E)  Methyltransferase 
A)  Elongation factor Tu 
B)  Aminoacyl tRNA synthetase 
C)  Nucleotidyltransferase. 
D)  Ribosomal protein S1. 
E)  Methyltransferase

Question 10

Which of the following has been used as a system to study brome mosaic virus replication?

Accepted Answer

A)  Bacterial cells 
B)  Yeast cells 
C)  Insect cells 
D)  Animal cells 
E)  Human cells 
A)  Bacterial cells 
B)  Yeast cells 
C)  Insect cells 
D)  Animal cells 
E)  Human cells

Question 11

The coat protein of cucumber mosaic virus contains a negatively charged domain localized on the internal side of the capsid.Which of the following describes the purpose of this domain?

Accepted Answer

A)  It maintains the structure of the capsid. 
B)  It associates with the positive charge of the viral RNA. 
C)  It is involved in release of the viral genome into the host cell. 
D)  It interacts with the host cell receptor. 
E)  It is involved in movement of the capsid across the plant cell wall. 
A)  It maintains the structure of the capsid. 
B)  It associates with the positive charge of the viral RNA. 
C)  It is involved in release of the viral genome into the host cell. 
D)  It interacts with the host cell receptor. 
E)  It is involved in movement of the capsid across the plant cell wall.

Question 12

Most cultivars of cucumber are resistant to the cucumber mosaic virus, which means they can be infected but do not show any symptoms of the infection.

Accepted Answer

A) True 
 B)False

Question 13

Plant defenses against RNA viruses involved which of the following?

Accepted Answer

A)  siRNAs 
B)  RISC 
C)  Dicer 
D)  Host RNA-dependent RNA polymerase 
E)  All of the above are correct. 
A)  siRNAs 
B)  RISC 
C)  Dicer 
D)  Host RNA-dependent RNA polymerase 
E)  All of the above are correct.

Question 14

Which of the following describes a feature of plant viruses, like cucumber mosaic virus, that is rare or absent in animal viruses?

Accepted Answer

A)  Segmented genomes are each packaged into a single virion. 
B)  Viral particles are transmitted to new hosts via an insect vector. 
C)  Viral genomes are composed of ssRNA with a negative sense. 
D)  Viral genomic RNA has a 5' cap. 
E)  Segmented genomes are each packaged into separate virions. 
A)  Segmented genomes are each packaged into a single virion. 
B)  Viral particles are transmitted to new hosts via an insect vector. 
C)  Viral genomes are composed of ssRNA with a negative sense. 
D)  Viral genomic RNA has a 5' cap. 
E)  Segmented genomes are each packaged into separate virions.

Question 15

All of the following describe a method of introducing Cucumber Mosaic Virus into a healthy plant EXCEPT….

Accepted Answer

A)  Grafting an infected branch 
B)  Insect feeding 
C)  Watering with a virus containing solution 
D)  Mechanical inoculation 
E)  Infection of seeds 
A)  Grafting an infected branch 
B)  Insect feeding 
C)  Watering with a virus containing solution 
D)  Mechanical inoculation 
E)  Infection of seeds

Question 16

How has reassortment played a role in the evolution of cucumber mosaic virus?

Accepted Answer

A)  It has allowed swapping of RNA segments between strains. 
B)  It has allowed swapping of regions of the genome between strains 
C)  It has increased mutations in the RNA genomes. 
D)  It has maintained stability of the viral genome. 
E)  It has increased genetic bottlenecks. 
A)  It has allowed swapping of RNA segments between strains. 
B)  It has allowed swapping of regions of the genome between strains 
C)  It has increased mutations in the RNA genomes. 
D)  It has maintained stability of the viral genome. 
E)  It has increased genetic bottlenecks.

Question 17

In cucumber mosaic virus, productive infection requires simultaneous entry of all three major RNA segments, each packaged into a separate virion.

Accepted Answer

A) True 
 B)False

Question 18

In an infected plants, the cucumber mosaic virus replicase complex is associated with the membrane surrounding which of the following cellular organelles?

Accepted Answer

A)  Golgi apparatus 
B)  Nucleus 
C)  Mitochondria 
D)  Central Vacuole 
E)  Chloroplast 
A)  Golgi apparatus 
B)  Nucleus 
C)  Mitochondria 
D)  Central Vacuole 
E)  Chloroplast

Question 19

Viruses that infect plants must contend with the thick cell wall, which is impedes movement of macromolecules between cells.Describe the mechanisms that cucumber mosaic virus uses to transverse this cell wall to both initiate an infection as well as spread through the plant.

Accepted Answer

Cucumber mosaic virus is transmitted to

Question 20

Which of the following describes the structures of the genomic RNA segments from cucumber mosaic virus?

Accepted Answer

A)  They have a 5' cap and a 3' poly A tail. 
B)  They have a 5' IRES and a 3' poly A tail. 
C)  They have a 5' cap and a 3' terminal tRNA-like structure. 
D)  They have a 5' phosphate and a 3' terminal tRNA-like structure. 
E)  They have a protein covalently bound to the 5' end and a 3' poly A tail. 
A)  They have a 5' cap and a 3' poly A tail. 
B)  They have a 5' IRES and a 3' poly A tail. 
C)  They have a 5' cap and a 3' terminal tRNA-like structure. 
D)  They have a 5' phosphate and a 3' terminal tRNA-like structure. 
E)  They have a protein covalently bound to the 5' end and a 3' poly A tail.

Exam 10: Cucumber Mosaic Virus

In lieu of an immune response, plants use RNA silencing to defend against virus infection.

The 5'-terminal 200nts on the cucumber mosaic virus genome function as a promoter for the synthesis of the minus-strand RNA.

Which of the following describes the hypersensitive response in plants infected with the cucumber mosaic virus?

All of the following describe a function of the movement protein from cucumber mosaic virus EXCEPT:

Which of the following statements about cucumber mosaic virus is FALSE?

Which of the following describes the first step in the replication cycle of the cucumber mosaic virus?

Which of the following proteins from cucumber mosaic virus acts at the RNA-dependent RNA polymerase?

What is the role of the plasmodesmata in the replication of cucumber mosaic virus?

Which of the following enzymes can transfer an amino acid to the 3' end of the genome of cucumber mosaic virus?

Which of the following has been used as a system to study brome mosaic virus replication?

The coat protein of cucumber mosaic virus contains a negatively charged domain localized on the internal side of the capsid.Which of the following describes the purpose of this domain?

Most cultivars of cucumber are resistant to the cucumber mosaic virus, which means they can be infected but do not show any symptoms of the infection.

Plant defenses against RNA viruses involved which of the following?

Which of the following describes a feature of plant viruses, like cucumber mosaic virus, that is rare or absent in animal viruses?

All of the following describe a method of introducing Cucumber Mosaic Virus into a healthy plant EXCEPT….

How has reassortment played a role in the evolution of cucumber mosaic virus?

In cucumber mosaic virus, productive infection requires simultaneous entry of all three major RNA segments, each packaged into a separate virion.

In an infected plants, the cucumber mosaic virus replicase complex is associated with the membrane surrounding which of the following cellular organelles?

Viruses that infect plants must contend with the thick cell wall, which is impedes movement of macromolecules between cells.Describe the mechanisms that cucumber mosaic virus uses to transverse this cell wall to both initiate an infection as well as spread through the plant.

Which of the following describes the structures of the genomic RNA segments from cucumber mosaic virus?

Introduction to Virology

Virus Structure and Assembly

Virus Classification: The World of Viruses

Virus Entry

Single-Stranded RNA Bacteriophages

Microviruses

Bacteriophage

Bacteriophage Lambda

Viruses of Archaea

Picornaviruses

Flaviviruses

Togaviruses

Coronaviruses

Paramyxo-Viruses and Rhabdoviruses

Filovirouses

Bunyaviruses

Influenza Viruses

Reoviruses

Parvoviruses

Polyomaviruses

Papillomaviruses

Adenoviruses

Herpesviruses

Baculoviruses

Poxviruses

Viruses of Algae and Mimivirus

Retroviruses

Human Immunodeficiency

Hepadnaviruses

Viroids and Hepatitis Delta Virus

Prions

Intrinsic Cellular Defenses Against Virus Infection

Innate and Adaptive Immune Responses to Virus Infection

Antiviral Vaccines

Antiviral Chemotherapy

Eukaryotic Virus Vectors

Filters