Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis

What do the Gram stain,acid-fast stain,and endospore stain have in common?

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Which of the following controls the amount of light entering the specimen?

Some microbes are not capable of growing on artificial media.

Which of the following will result when 1% to 5% agar is added to nutrient broth,boiled,and cooled?

Which of the following characteristics refers to the microscope's ability to show two separate entities as separate and distinct?

A rod-shaped bacterium is measured as 0.3 micrometers ( $\mu$ m)in length using an ocular micrometer.Your instructor wants you to report the length in millimeters (mm)to test your understanding of metric conversions.What is the length of the organism in millimeters?

A confocal scanning microscope

The Gram staining procedure is best described as a(n)__ staining technique.

A nutrient medium that has all of its chemical components identified,and their precise concentrations known and reproducible,would be termed

The three physical forms of laboratory media are

Please read the clinical scenario, and then answer the question that follow to become familiar with the traditional NCLEX question format. You begin your shift at the inner city outreach clinic when a young woman enters crying. She is 19 years old and 28 weeks pregnant with her second child. Her complaint is that she woke this morning leaking clear to milky-colored fluid vaginally. Her first child was born 6 weeks early due to premature rupture of membranes and she is worried this is happening again. You reassure the patient, and explain that a vaginal speculum exam will be performed and specimens will be taken for examination. Once obtaining the proper specimens, you label them appropriately, and send the wet mount and culturette to the laboratory for processing. -You understand that a microscopic view is needed to visualize microbes that fall within the 1-10 µm micrometer size range but also to detect characteristic ferning of amniotic fluid.Which type of microscope will be used by the laboratory technicians to make these observations of the patient sample?

Please read the clinical scenario, and then answer the question that follow to become familiar with the traditional NCLEX question format. Mr. Nowak is a homeless patient who presented to your clinic today with an injury to his left arm. He injured it on an old piece of scrap metal. His wound is red, hot, and tender with purulent drainage. You swab the site and send it to the laboratory for microbial analysis. -Although microscopic analysis revealed the presence of grapelike clusters of gram-positive cocci,you know that the species identification of the organism will require biochemical testing.Biochemical tests aid in microbial identification by providing information on

Please read the clinical scenario, and then answer the question that follow to become familiar with the traditional NCLEX question format. Mr. Nowak is a homeless patient who presented to your clinic today with an injury to his left arm. He injured it on an old piece of scrap metal. His wound is red, hot, and tender with purulent drainage. You swab the site and send it to the laboratory for microbial analysis. -Cultures and specimens pose a potential employee and public health hazard,and require proper handling and disposal via specific medical waste policies.Some facilities,such as the ATCC,are regulated to maintain living catalogs of specimens for research and educational purposes.Such collections are referred to as

Normal incubation temperatures range from 30 ^$\circ$ C to 60 ^$\circ$ C.

One colony typically develops from the growth of several parent bacterial cells.

Which type of microscope is the most widely used and shows cells against a bright background?

Please read the clinical scenario, and then answer the question that follow to become familiar with the traditional NCLEX question format. You begin your shift at the inner city outreach clinic when a young woman enters crying. She is 19 years old and 28 weeks pregnant with her second child. Her complaint is that she woke this morning leaking clear to milky-colored fluid vaginally. Her first child was born 6 weeks early due to premature rupture of membranes and she is worried this is happening again. You reassure the patient, and explain that a vaginal speculum exam will be performed and specimens will be taken for examination. Once obtaining the proper specimens, you label them appropriately, and send the wet mount and culturette to the laboratory for processing. -No ferning of the vaginal fluid is detected,and you reassure your patient that her membranes have not ruptured.You explain that the laboratory technicians will use which five basic techniques (in order)to manipulate,grow,examine,and characterize any microorganisms present in the collected specimens?

Which of the following is not an inoculating tool?

Which of the following is essential for development of discrete,isolated colonies?