Question 1

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens.

Accepted Answer

A) True 
 B)False

Question 2

A reducing media is used to culture

Accepted Answer

A) fastidious organisms. 
B) aerobic organisms. 
C) anaerobic organisms. 
D) any pathogenic organisms. 
E) None of the choices is correct. 
A) fastidious organisms. 
B) aerobic organisms. 
C) anaerobic organisms. 
D) any pathogenic organisms. 
E) None of the choices is correct.

Question 3

Which type of microscope does not use light in forming the specimen image?

Accepted Answer

A) bright field 
B) dark field 
C) phase contrast 
D) fluorescence 
E) electron 
A) bright field 
B) dark field 
C) phase contrast 
D) fluorescence 
E) electron

Question 4

Which of the following magnifies the specimen to produce its real image?

Accepted Answer

A) condenser 
B) objective lens 
C) ocular lens 
D) body 
E) nosepiece 
A) condenser 
B) objective lens 
C) ocular lens 
D) body 
E) nosepiece

Question 5

Which type of microscope achieves the greatest resolution and highest magnification?

Accepted Answer

A) bright field 
B) dark field 
C) phase contrast 
D) fluorescence 
E) electron 
A) bright field 
B) dark field 
C) phase contrast 
D) fluorescence 
E) electron

Question 6

The bending of light rays as they pass from one medium to another is called refraction.

Accepted Answer

A) True 
 B)False

Question 7

At the end of the Gram stain,gram-positive bacteria will be seen as red/pink cells.

Accepted Answer

A) True 
 B)False

Question 8

The purpose of staining cells on a microscope slide is to

Accepted Answer

A) kill them. 
B) secure them to the slide. 
C) enlarge the cells. 
D) add contrast in order to see them better. 
E) see motility. 
A) kill them. 
B) secure them to the slide. 
C) enlarge the cells. 
D) add contrast in order to see them better. 
E) see motility.

Question 9

Please read the clinical scenario, and then answer the question that follow to become familiar with the traditional NCLEX question format.
  You begin your shift at the inner city outreach clinic when a young woman enters crying. She is 19 years old and 28 weeks pregnant with her second child. Her complaint is that she woke this morning leaking clear to milky-colored fluid vaginally. Her first child was born 6 weeks early due to premature rupture of membranes and she is worried this is happening again. You reassure the patient, and explain that a vaginal speculum exam will be performed and specimens will be taken for examination. Once obtaining the proper specimens, you label them appropriately, and send the wet mount and culturette to the laboratory for processing.
-The patient asks how microbes from her body can be grown in the lab.You explain that specimens are introduced to nutrient medium and that any growth of the microbe that appears after incubating the specimen is called the

Accepted Answer

A) colony. 
B) culture. 
C) microorganism. 
D) infectious agent. 
A) colony. 
B) culture. 
C) microorganism. 
D) infectious agent.

Question 10

An enveloped virus measures 0.02 micrometers ($\mu$m)in diameter.What is the diameter of this virus in nanometers (nm)?

Accepted Answer

A) 2 nm 
B) 20 nm 
C) 0.00002 nm 
D) 0.2 nm 
A) 2 nm 
B) 20 nm 
C) 0.00002 nm 
D) 0.2 nm

Question 11

The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the

Accepted Answer

A) colony. 
B) inoculum. 
C) streak. 
D) loop. 
E) None of the choices is correct. 
A) colony. 
B) inoculum. 
C) streak. 
D) loop. 
E) None of the choices is correct.

Question 12

Agar is an important component of media because

Accepted Answer

A) bacteria require agar to grow. 
B) agar inhibits mold growth. 
C) agar provides a solid surface for bacterial growth. 
D) agar prevents contamination. 
E) All of the choices are correct. 
A) bacteria require agar to grow. 
B) agar inhibits mold growth. 
C) agar provides a solid surface for bacterial growth. 
D) agar prevents contamination. 
E) All of the choices are correct.

Question 13

The procedures for culturing a microorganism require the use of a microscope.

Accepted Answer

A) True 
 B)False

Question 14

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Accepted Answer

A) differential 
B) selective 
C) enumeration 
D) enriched 
E) reducing 
A) differential 
B) selective 
C) enumeration 
D) enriched 
E) reducing

Question 15

All of the following are examples of different types of microbiological media except

Accepted Answer

A) broth. 
B) enriched. 
C) agar. 
D) petri dish. 
E) selective. 
A) broth. 
B) enriched. 
C) agar. 
D) petri dish. 
E) selective.

Question 16

A common medium used for growing fastidious bacteria is

Accepted Answer

A) blood agar. 
B) trypticase soy agar. 
C) mannitol salt agar. 
D) MacConkey medium. 
E) a reducing medium. 
A) blood agar. 
B) trypticase soy agar. 
C) mannitol salt agar. 
D) MacConkey medium. 
E) a reducing medium.

Question 17

Which type of microscope bombards a whole,metal-coated specimen with electrons moving back and forth over it?

Accepted Answer

A) fluorescence 
B) differential interference contrast 
C) scanning electron 
D) transmission electron 
E) phase contrast 
A) fluorescence 
B) differential interference contrast 
C) scanning electron 
D) transmission electron 
E) phase contrast

Question 18

Which type of media would be the best choice when shipping a sample of bacteria to a laboratory to be tested from a satellite office site?

Accepted Answer

A) transport 
B) EMB 
C) blood 
D) thioglycollate 
E) general purpose 
A) transport 
B) EMB 
C) blood 
D) thioglycollate 
E) general purpose

Question 19

A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

Accepted Answer

A) True 
 B)False

Question 20

For which bacterial genus does mannitol salt agar differentiate between species?

Accepted Answer

A) Salmonella 
B) Streptococcus 
C) Neisseria 
D) Staphylococcus 
E) Escherichia 
A) Salmonella 
B) Streptococcus 
C) Neisseria 
D) Staphylococcus 
E) Escherichia

Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens.

A reducing media is used to culture

Which type of microscope does not use light in forming the specimen image?

Which of the following magnifies the specimen to produce its real image?

Which type of microscope achieves the greatest resolution and highest magnification?

The bending of light rays as they pass from one medium to another is called refraction.

At the end of the Gram stain,gram-positive bacteria will be seen as red/pink cells.

The purpose of staining cells on a microscope slide is to

An enveloped virus measures 0.02 micrometers ( μ\muμ m)in diameter.What is the diameter of this virus in nanometers (nm)?

The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the

Agar is an important component of media because

The procedures for culturing a microorganism require the use of a microscope.

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

All of the following are examples of different types of microbiological media except

A common medium used for growing fastidious bacteria is

Which type of microscope bombards a whole,metal-coated specimen with electrons moving back and forth over it?

Which type of media would be the best choice when shipping a sample of bacteria to a laboratory to be tested from a satellite office site?

A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

For which bacterial genus does mannitol salt agar differentiate between species?

Introduction to Microbes and Their Building Blocks

Bacteria and Archaea

Eukaryotic Cells and Microorganisms

Viral Structure and Life Cycles

Microbial Nutrition and Growth

Microbial Metabolism

Microbial Genetics and Genetic Engineering

Physical and Chemical Control of Microbes

Antimicrobial Treatment

Interactions Between Microbes and Humans

Host Defenses I: Overview and Nonspecific Defenses

Host Defenses II: Specific Immunity and Immunization

Disorders in Immunity

Diagnosing Infections

Infectious Diseases Affecting the Skin and Eyes

Infectious Diseases Affecting the Nervous System

Infectious Diseases Affecting the Cardiovascular and Lymphatic Systems

Infectious Diseases Affecting the Respiratory Systems

Infectious Diseases Affecting the Gastrointestinal Tract

Infectious Diseases Affecting the Genitourinary System

One Health: The Interconnected Health of the Environment, humans, and Other Animals

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An enveloped virus measures 0.02 micrometers ( $\mu$ m)in diameter.What is the diameter of this virus in nanometers (nm)?