Exam 12: Recombinant Dna, Cloning, Chimeric Genes, and Synthetic Biology

In the Southern hybridization procedure, the gel after electrophoresis is treated with NaOH and then neutralized before blotting. What is the primary function of the alkaline treatment?

In vitro ____ makes it possible to alter the nucleotide sequence of a cloned gene systematically.

What would be the 9-residue primer used to amplify: 5'-ATCGACGTTACGCTACATAGCATAAGGCTT-3'

All are steps in the sequence for construction of a chimeric plasmid EXCEPT:

Define a probe and explain how probes are used in colony hybridization experiments.​

All are features of a plasmid useful for cloning EXCEPT:

PCR-based mutagenesis is used to create:

Which of the following is the appropriate source of the DNA polymerase included in the PCR reaction mixture?

Each cycle of amplification in PCR involves all of the steps EXCEPT:

A genomic DNA library is:

All are characteristic of YACs (yeast artificial chromosomes) EXCEPT:

A method used to insert or transform cells with a plasmid is to:

How does RNA interference function?

Which of the following describes the major utility of a eukaryotic cDNA library if one is trying to express a protein present in a eukaryotic cell?

Explain how the directed evolution of horseradish peroxidase was developed through high-throughput protein screening.​

To express a eukaryotic protein in E. coli, the eukaryotic cDNA must be cloned in a(n) ____ that contains ____ and ____.

To position a gene in an expression vector downstream from a promoter (i.e., perform directional cloning), it is best to: