Question 1

The most common way to follow bacterial transformation with a plasmid is by

Accepted Answer

A) manufacturing the bacterial protein. 
B) conferring antibiotic resistance. 
C) separating the altered cell surface. 
D) making the cells immortal. 
E) altering the physical appearance of the cells.

Question 2

Which of the following types of information would be most useful in an effort to move a DNA fragment from one plasmid vector to another,using molecular cloning techniques?

Accepted Answer

A) DNA fingerprint data 
B) Sequence of PCR primers 
C) Data from cloning of Dolly the sheep 
D) Restriction maps of the plasmids

Question 3

Restriction enzymes are

Accepted Answer

A) proteases. 
B) lipases. 
C) endonucleases. 
D) exonucleases.

Question 4

Both DNA replication and PCR require: (check all that apply)

Accepted Answer

A) DNA polymerase. 
B) DNA helicase. 
C) a DNA template. 
D) a thermocycler machine. 
E) plasmid DNA.

Question 5

Bacterial plasmids and artificial chromosomes are used as ________ to insert foreign DNA into host cells and create recombinant genomes.

Accepted Answer

A) clones 
B) vectors 
C) endonucleases 
D) subunits

Question 6

Select the experiments that can be characterized as reverse genetics: (check all that apply)

Accepted Answer

A) Generation and analysis of a knockout mouse 
B) Random mutagenesis of an organism,then selecting mutants by phenotype 
C) RNA interference of a gene in C.elegans 
D) Using reverse transcriptase to create a cDNA library

Question 7

Crop plants have been protected from certain insects by the addition of a gene for a specific insect toxin from the bacterium

Accepted Answer

A) Agrobacterium tumefaciens. 
B) Bacillus thuringiensis. 
C) Herpes simplex. 
D) Pseudomonas. 
E) tobacco mosaic.

Question 8

Which component of the PCR mix do researchers manipulate to induce a site-specific mutation?

Accepted Answer

A) Primers 
B) Taq polymerase 
C) DNA template 
D) Reverse transcriptase

Exam 17: Biotechnology