In Sanger Sequencing, What Causes DNA Synthesis to Terminate at a Specific

Multiple Choice

In Sanger sequencing, what causes DNA synthesis to terminate at a specific base?

A) Restriction endonucleases cleave DNA after particular bases.
B) Fluorescent molecules produce high-energy light that inactivates DNA polymerase.
C) Incorporation of a deoxynucleotide that lacks a base causes DNA polymerase to stall.
D) Incorporation of a dideoxynucleotide that lacks a 3′ hydroxyl.
E) Incorporation of a ribonucleotide that lacks a 5' phosphate.