A Scientist Edits a Mammalian Genome by Inserting a GFP

Multiple Choice

A scientist edits a mammalian genome by inserting a GFP reporter sequence downstream of a gene of interest. Which of the following methods is she likely to have used to make this change?

A) site-directed mutagenesis
B) oligonucleotide-directed mutagenesis
C) CRISPR-Cas9 genome editing followed by nonhomologous end joining (NHEJ)
D) CRISPR-Cas9 genome editing followed by homologous recombination (HR) with a donor template
E) mutagenesis with radiation