Question 1

PCR sequence specific oligonucleotide probe methods are usually preferred for large volumes of specimens over PCR sequence specific primer methods.

Accepted Answer

A) True 
 B)False

Question 2

It is still normal a year after progenitor cell transplant to perform STR analysis and find both donor and recipient DNA in peripheral blood white cells.

Accepted Answer

A) True 
 B)False

Question 3

When DNA has been extracted from a sample for HLA molecular typing

Accepted Answer

A) it must be amplified immediately to insure accuracy of the process. 
B) it can be stored one month at -20° C. 
C) it can be stored in the refrigerator no more than 24 hours prior to amplification. 
D) it can be stored indefinitely at -70° C.

Question 4

What is a short tandem repeat (STR), and how can it be used to determine progenitor cell engraftment?

Accepted Answer

The answer of What is a short tandem repeat (STR),...

Question 5

What is an important advantage of HLA testing by molecular methods?

Accepted Answer

A) Molecular methods are inexpensive. 
B) Many alleles thought to be different based on serological methods have actually been shown by molecular methods to be the same. 
C) There is more lot- to- lot variability in antisera than there is in molecular oligonucleotide primers. 
D) Although cells used for typing must still be viable, fewer cells are required to get an accurate typing.

Question 6

HLA typing is acceptable on lymphocyte preparations in which >95% of the cells can ingest the dye ________ ________.

Accepted Answer

The answer of HLA typing is acceptable on lymphocyte preparations...

Question 7

How can the sensitivity of the cross- match between the recipient's serum and the donor's cells be enhanced?

Accepted Answer

The answer of How can the sensitivity of the cross-...

Question 8

Which process below includes all the controls utilized to make sure that a particular laboratory analysis is performing properly?

Accepted Answer

A) Continuous quality improvement (CQI) 
B) Quality assurance (Q
A)  
C) Quality control (Q
C)  
D) Total quality management (TQM)

Question 9

If a transplant is going to fail due to immune attack, it will be because of recipient production of antibodies or cytotoxic T cells.

Accepted Answer

A) True 
 B)False

Question 10

The complement dependent cytotoxicity method of HLA testing can be used for

Accepted Answer

A) cross- matching recipient plasma with donor cells. 
B) HLA typing of Class I antigens. 
C) identifying recipient HLA antibodies. 
D) HLA typing of Class II antigens. 
E) All of the above.

Question 11

Gels for HLA typing by PCR should be interpreted by at least________ (how many?)people.

Accepted Answer

The answer of Gels for HLA typing by PCR should...

Question 12

Traditional HLA typing always requires viable cells.

Accepted Answer

A) True 
 B)False

Question 13

Personnel who work in HLA laboratories earn certification from the

Accepted Answer

A) National Credentialing Agency for Laboratory Personnel. 
B) American Society of Clinical Pathologists. 
C) American Board of Histocompatibility and Immunogenetics. 
D) American Association of Blood Banks. 
E) All of the above.

Question 14

For CLIA compliance, HLA testing labs can be inspected by

Accepted Answer

A) College of American Pathologists (CAP). 
B) American Association of Blood Banks (AAB
B) . 
C) Centers for Medicare and Medicaid Services. 
D) American Society of Histocompatibility and Immunogenetics (ASHI). 
E) All of the above.

Question 15

Which of the following is (are)TRUE regarding acceptable specimens for HLA typing by serological methods?

Accepted Answer

A) Lithium heparin is the anticoagulant of choice for HLA typing. 
B) Peripheral blood, spleen tissue, and lymph node tissue can all be used for HLA typing. 
C) Specimens should be stored at 2-6° C after collection. 
D) Specimens preserved in sodium heparin or acid citrate dextrose are acceptable for up to one week after collection. 
E) All of the above.

Question 16

An advantage of flow cytometry cross- matching is that B cells and T cells do not have to be separated in lymphocyte preparations prior to analysis.

Accepted Answer

A) True 
 B)False

Question 17

For HLA typing, the cell of choice is the ________ .

Accepted Answer

The answer of For HLA typing, the cell of choice...

Question 18

If a unrelated donor is being considered for progenitor cell transplant, which molecular HLA typing method below is required before final transplant can occur?

Accepted Answer

A) Low- resolution PCR sequence specific oligonucleotide primer typing 
B) High- resolution PCR sequence specific oligonucleotide primer typing 
C) Sequence- based typing 
D) High- resolution PCR sequence specific probe typing 
E) Low- resolution PCR sequence specific probe typing

Question 19

How can monocytes be removed from lymphocyte preparations?

Accepted Answer

A) Washing Ficoll- Hypaque density separation with 5% ethanol 
B) Specific monocyte destruction with monoclonal antibodies 
C) Adding iron filings for monocyte phagocytosis 
D) A and B 
E) B and C

Question 20

After progenitor cell transplant, if STR DNA regions from both donor and recipient are detected, this is called a genetic ________.

Accepted Answer

The answer of After progenitor cell transplant, if STR DNA...

Exam 15: Histocompatibility Laboratory Techniques and Applications

PCR sequence specific oligonucleotide probe methods are usually preferred for large volumes of specimens over PCR sequence specific primer methods.

It is still normal a year after progenitor cell transplant to perform STR analysis and find both donor and recipient DNA in peripheral blood white cells.

When DNA has been extracted from a sample for HLA molecular typing

What is a short tandem repeat (STR), and how can it be used to determine progenitor cell engraftment?

What is an important advantage of HLA testing by molecular methods?

HLA typing is acceptable on lymphocyte preparations in which >95% of the cells can ingest the dye .

How can the sensitivity of the cross- match between the recipient's serum and the donor's cells be enhanced?

Which process below includes all the controls utilized to make sure that a particular laboratory analysis is performing properly?

If a transplant is going to fail due to immune attack, it will be because of recipient production of antibodies or cytotoxic T cells.

The complement dependent cytotoxicity method of HLA testing can be used for

Gels for HLA typing by PCR should be interpreted by at least________ (how many?)people.

Traditional HLA typing always requires viable cells.

Personnel who work in HLA laboratories earn certification from the

For CLIA compliance, HLA testing labs can be inspected by

Which of the following is (are) TRUE regarding acceptable specimens for HLA typing by serological methods?

An advantage of flow cytometry cross- matching is that B cells and T cells do not have to be separated in lymphocyte preparations prior to analysis.

For HLA typing, the cell of choice is the ________ .

If a unrelated donor is being considered for progenitor cell transplant, which molecular HLA typing method below is required before final transplant can occur?

How can monocytes be removed from lymphocyte preparations?

After progenitor cell transplant, if STR DNA regions from both donor and recipient are detected, this is called a genetic ________.