Question 1

Which of the following is not acceptable for specimens to be used for HLA typing?

Accepted Answer

A) ACD anticoagulant 
B) Refrigerator storage up to 14 days 
C) Room temperature storage up to five days 
D) EDTA anticoagulant 
E) Heparin anticoagulant

Question 2

Class II HLA antigens are best determined from

Accepted Answer

A) monocytes. 
B) T lymphocytes. 
C) B lymphocytes. 
D) neutrophils.

Question 3

The Ficoll Hypaque technique isolates mononuclear cells by

Accepted Answer

A) cell surface marker affinity. 
B) cellular adherence. 
C) cell membrane solubility. 
D) cell density.

Question 4

A lymphocyte preparations in which < 95% of the cells have ingested trypan blue must be discarded and not used for HLA typing.

Accepted Answer

A) True 
 B)False

Question 5

Under the Clinical Laboratory Improvement Amendments (CLIA)regulations, HLA testing is considered

Accepted Answer

A) low complexity. 
B) high complexity. 
C) waived. 
D) moderate complexity.

Exam 15: Histocompatibility Laboratory Techniques and Applications

Which of the following is not acceptable for specimens to be used for HLA typing?

Class II HLA antigens are best determined from

The Ficoll Hypaque technique isolates mononuclear cells by

A lymphocyte preparations in which < 95% of the cells have ingested trypan blue must be discarded and not used for HLA typing.

Under the Clinical Laboratory Improvement Amendments (CLIA) regulations, HLA testing is considered