How Is Tandem Affinity Purification Distinct from Other Purification Schemes

Multiple Choice

How is tandem affinity purification distinct from other purification schemes such as simple affinity or ion-exchange chromatography?

A) In its use of antibodies
B) In its use of proteases
C) In its use of glutathione S-transferase
D) In its use of viral peptides
E) In its use of limited washing steps