In Sanger Sequencing, Does Each Labeled DNA Molecule Have a Fluorescently

Multiple Choice

In Sanger sequencing, does each labeled DNA molecule have a fluorescently labeled dideoxyribonucleotide at its 5′ end or its 3′ end? Do the labeled nucleotides lack the 2′ or 3′ hydroxyl group?

A) 3′ end; 2′ hydroxyl group only
B) 3′ end; 3′ hydroxyl group only
C) 5′ end; 2′ hydroxyl group only
D) 5′ end; 3′ hydroxyl group only
E) 3′ end; both hydroxyl groups