The Binding of Protein a to Two Other Proteins, B

Multiple Choice

The binding of protein A to two other proteins, B and C, has been studied with fluorescence anisotropy, and the results of the measurements have been plotted in the following schematic diagram. Given equal kₒn rate constants, which complex do you think has a longer half-life? What is the value of K?ₐ (the association constant) for this complex?

A) A-B; 10⁵ L/mol
B) A-B; 10-⁵ L/mol
C) A-C; 1.5 × 10⁵ L/mol
D) A-C; 6.7 × 10⁴ L/mol
E) A-C; 6.7 × 10-⁴ L/mol