Indicate Whether Each of the Following Manipulations or Procedures Used

Short Answer

Indicate whether each of the following manipulations or procedures used in current recombinant DNA technology commonly rely on an enzyme (E) or are done nonenzymatically (N). Your answer would be a six-letter string composed of letters E and N only, e.g. EEEEEE.
( ) Cleavage of DNA at specific sites
( ) DNA ligation
( ) DNA cloning
( ) Nucleic acid hybridization
( ) Synthesis of DNA of any desired specific sequence
( ) DNA sequencing