Your Friend Has Cut a 3000-Nucleotide-Pair Circular Plasmid with Either

Multiple Choice

Your friend has cut a 3000-nucleotide-pair circular plasmid with either or both restriction enzymes A and B, and has separated the digestion products by electrophoresis. The results are shown in the schematic drawing below, with the approximate size of the fragments indicated above each band. Based on these results, what is the closest distance (in nucleotide pairs) between an A restriction site and a B restriction site on this plasmid?

A) 400
B) 600
C) 1000
D) 1200
E) 1600