Which of the Following Is the Correct Order of Steps

Multiple Choice

Which of the following is the correct order of steps when performing a Southern blot after isolation of the DNA?

A) Probe hybridization, restriction enzyme digestion, denaturation, electrophoresis
B) Denaturation, electrophoresis, probe hybridization, restriction enzyme digestion
C) Restriction enzyme digestion, electrophoresis, denaturation, probe hybridization
D) Electrophoresis, restriction enzyme digestion, probe hybridization, denaturation