A Chemical Cross-Linking Reagent Has Two Reactive Ends That Can

Multiple Choice

A chemical cross-linking reagent has two reactive ends that can be used to covalently link two different amino acid groups in proteins.The links that result are similar to a disulfide bridge that forms between two cysteine groups.Biochemists use chemical cross-linking reagents to test for conformational changes in proteins because the cross-links often freeze proteins in a single conformation.Which method of increasing reaction rates would be most adversely affected by chemical cross-linking?

A) Metal ion catalysis
B) Covalent catalysis
C) Acid-base catalysis
D) Induced-fit mechanism
E) Substrate orientation