If Negatively Supercoiled Plasmid DNA Is Mixed with Human Topoisomerase

Multiple Choice

If negatively supercoiled plasmid DNA is mixed with human topoisomerase I and ATP, which effect would be observed?

A) Rapid ATP hydrolysis would occur.
B) The linking number for the plasmid would increase.
C) The writhe on the plasmid would increase.
D) The superhelical density would become more negative.
E) None of these effects would be observed.