Question 1

Nonselective media supports

Accepted Answer

A)  the grouping of microbes based on different characteristics demonstrated in the media. 
B)  the growth of one type or group of microbes, but not another. 
C)  the growth of most nonfastidious microbes. 
D)  the growth of fastidious microbes through the addition of certain growth enhancers. 
A)  the grouping of microbes based on different characteristics demonstrated in the media. 
B)  the growth of one type or group of microbes, but not another. 
C)  the growth of most nonfastidious microbes. 
D)  the growth of fastidious microbes through the addition of certain growth enhancers. C

Question 2

What work practice have microbiologists incorporated to keep laboratory testing cost-effective while providing optimum patient care?

Accepted Answer

A)  Using definitive identification for the standard of quality patient care 
B)  Always identifying organisms to the maximum extent 
C)  Limited identification procedures 
D)  Limited antimicrobial susceptibility procedures 
A)  Using definitive identification for the standard of quality patient care 
B)  Always identifying organisms to the maximum extent 
C)  Limited identification procedures 
D)  Limited antimicrobial susceptibility procedures C

Question 3

The requisition form should provide all the following information, except

Accepted Answer

A)  patient name or identification number. 
B)  patient age and gender. 
C)  patient home address. 
D)  specific anatomic site. 
A)  patient name or identification number. 
B)  patient age and gender. 
C)  patient home address. 
D)  specific anatomic site. C

Question 4

All the following principles of specimen collection are fundamental to ensuring appropriate specimen management, except:

Accepted Answer

A)  Collect the appropriate quantity of specimen. 
B)  Label the specimen accurately with the specific anatomic site and the patient information. 
C)  Select the correct anatomic site to collect the specimen. 
D)  It is acceptable to delay transport of the specimen to the laboratory if it is in transport media. 
A)  Collect the appropriate quantity of specimen. 
B)  Label the specimen accurately with the specific anatomic site and the patient information. 
C)  Select the correct anatomic site to collect the specimen. 
D)  It is acceptable to delay transport of the specimen to the laboratory if it is in transport media.

Question 5

The purpose of transport media is to

Accepted Answer

A)  make sure the microorganisms can multiply and live as though they were still in the host. 
B)  keep the swab moist so that the microorganisms do not dry out. 
C)  ensure the preservation of microorganisms in the specimen. 
D)  take the place of plating a routine culture with the specimen. 
A)  make sure the microorganisms can multiply and live as though they were still in the host. 
B)  keep the swab moist so that the microorganisms do not dry out. 
C)  ensure the preservation of microorganisms in the specimen. 
D)  take the place of plating a routine culture with the specimen.

Question 6

To assist hospital personnel in collecting the highest quality specimen, the laboratory should

Accepted Answer

A)  post the microbiology laboratory's phone numbers in each section so personnel can call with questions and problems. 
B)  develop a well-written handbook and make it available at every patient care unit. 
C)  allow personnel to go to the floor and collect all specimens. 
D)  severely reprimand staff for not collecting specimens properly. 
A)  post the microbiology laboratory's phone numbers in each section so personnel can call with questions and problems. 
B)  develop a well-written handbook and make it available at every patient care unit. 
C)  allow personnel to go to the floor and collect all specimens. 
D)  severely reprimand staff for not collecting specimens properly.

Question 7

Sputum specimens are often collected for the diagnosis of

Accepted Answer

A)  acute pharyngitis. 
B)  bacterial pneumonia. 
C)  meningitis. 
D)  diverticulitis. 
A)  acute pharyngitis. 
B)  bacterial pneumonia. 
C)  meningitis. 
D)  diverticulitis.

Question 8

A night technician is working in microbiology when a cerebrospinal fluid (CSF) specimen comes in. Almost simultaneously, the technician is called to the emergency department to draw blood on seriously injured car crash victims. How would the technician store the CSF until time permits to work on the CSF specimen?

Accepted Answer

A)  Put the specimen in the refrigerator where it is good for 4 hours at 4° C. 
B)  Leave the specimen on the shelf where it is good for 6 hours at room temperature. 
C)  Pour the specimen into a tube of broth. 
D)  Place the specimen in a 35° C incubator where it is good for up to 6 hours. 
A)  Put the specimen in the refrigerator where it is good for 4 hours at 4° C. 
B)  Leave the specimen on the shelf where it is good for 6 hours at room temperature. 
C)  Pour the specimen into a tube of broth. 
D)  Place the specimen in a 35° C incubator where it is good for up to 6 hours.

Question 9

When using the isolation streaking technique, the most isolated forms of the organism should be in which quadrant?

Accepted Answer

A)  Fourth 
B)  Third 
C)  Second 
D)  First 
A)  Fourth 
B)  Third 
C)  Second 
D)  First

Question 10

Data generated by the laboratory is directly influenced by

Accepted Answer

A)  the quality of the specimen and its condition when received. 
B)  the physician's decision to do the test. 
C)  quality control performed on a monthly basis. 
D)  the air quality in the laboratory. 
A)  the quality of the specimen and its condition when received. 
B)  the physician's decision to do the test. 
C)  quality control performed on a monthly basis. 
D)  the air quality in the laboratory.

Question 11

How should specimen collection instructions be given to the patient to ensure collection of a good specimen for culture?

Accepted Answer

A)  Written only in English 
B)  Verbally, in many different languages 
C)  Verbally, only in English 
D)  Both verbal and written instruction in simple language 
A)  Written only in English 
B)  Verbally, in many different languages 
C)  Verbally, only in English 
D)  Both verbal and written instruction in simple language

Question 12

Proper identification of each specimen includes a label firmly attached to the container with all the following information, except

Accepted Answer

A)  diagnosis. 
B)  name. 
C)  identification number. 
D)  date. 
A)  diagnosis. 
B)  name. 
C)  identification number. 
D)  date.

Question 13

What is the primary goal in the transportation of specimens to the laboratory?

Accepted Answer

A)  To get the specimen to the laboratory by the end of the day 
B)  To maintain the specimen as near its original state as possible with minimal deterioration 
C)  To place the specimen in formalin and then transport it to the laboratory 
D)  To allow the specimen to sit, as long as it is delivered to the laboratory within 2 to 3 days after collection 
A)  To get the specimen to the laboratory by the end of the day 
B)  To maintain the specimen as near its original state as possible with minimal deterioration 
C)  To place the specimen in formalin and then transport it to the laboratory 
D)  To allow the specimen to sit, as long as it is delivered to the laboratory within 2 to 3 days after collection

Question 14

Routine primary plating media include all the following items, except

Accepted Answer

A)  special biochemical media. 
B)  nonselective agar plate. 
C)  selective and differential medium for enteric gram-negative bacilli for most routine bacterial cultures. 
D)  broth medium used as a supplement. 
A)  special biochemical media. 
B)  nonselective agar plate. 
C)  selective and differential medium for enteric gram-negative bacilli for most routine bacterial cultures. 
D)  broth medium used as a supplement.

Question 15

Notations from the macroscopic (gross) examination of a specimen should include all the following, except

Accepted Answer

A)  swab or aspirate. 
B)  stool consistency. 
C)  blood or mucus present. 
D)  what type of container the specimen was collected in. 
A)  swab or aspirate. 
B)  stool consistency. 
C)  blood or mucus present. 
D)  what type of container the specimen was collected in.

Question 16

Specimens such as blood, bone marrow, and synovium are mixed with anticoagulants right after collection. Why should this occur?

Accepted Answer

A)  Anticoagulants often dissolve part of the bacteria's cell wall and congeal many bacteria into groups. 
B)  Organisms become bound up in the clotted material and are difficult to isolate. 
C)  It ensures the specimen will work when using an automated spreader device. 
D)  It kills off the normal flora and only leaves the pathogens in the specimen. 
A)  Anticoagulants often dissolve part of the bacteria's cell wall and congeal many bacteria into groups. 
B)  Organisms become bound up in the clotted material and are difficult to isolate. 
C)  It ensures the specimen will work when using an automated spreader device. 
D)  It kills off the normal flora and only leaves the pathogens in the specimen.

Question 17

Why is clean-catch midstream urine used for a urine culture as opposed to a clean-catch urine?

Accepted Answer

A)  The first portion of the urine flow washes contaminants from the urethra and the next portion of urine is more representative of the bladder. 
B)  The name was changed, but the procedure remains the same and the entire amount of urine from the bladder is cultured. 
C)  No urine is free from contamination, so just wipe the external genitalia and void into the cup. 
D)  Catheterized specimens are also called clean-catch midstream urine specimen. 
A)  The first portion of the urine flow washes contaminants from the urethra and the next portion of urine is more representative of the bladder. 
B)  The name was changed, but the procedure remains the same and the entire amount of urine from the bladder is cultured. 
C)  No urine is free from contamination, so just wipe the external genitalia and void into the cup. 
D)  Catheterized specimens are also called clean-catch midstream urine specimen.

Question 18

Types of specimens that can be batch processed, including all the following, except

Accepted Answer

A)  specimens for fastidious organisms. 
B)  specimens for acid-fast bacillus (AF
B)  cultures. 
C)  stool specimens for ova and parasite tests that are collected in preservatives. 
D)  specimens for viral culture collected in viral transport media. 
A)  specimens for fastidious organisms. 
B)  specimens for acid-fast bacillus (AF
B)  cultures. 
C)  stool specimens for ova and parasite tests that are collected in preservatives. 
D)  specimens for viral culture collected in viral transport media.

Question 19

Most bacterial cultures are incubated at this temperature range.

Accepted Answer

A)  25° C to 27° C 
B)  30° C to 40° C 
C)  35° C to 37° C 
D)  40° C to 42° C 
A)  25° C to 27° C 
B)  30° C to 40° C 
C)  35° C to 37° C 
D)  40° C to 42° C

Question 20

Selective media

Accepted Answer

A)  allow the growth of fastidious microbes through the addition of certain growth enhancers. 
B)  support the grouping of microbes based on different characteristics demonstrated in the media. 
C)  support the growth of most nonfastidious microbes. 
D)  support the growth of one type or group of microbes, but not another. 
A)  allow the growth of fastidious microbes through the addition of certain growth enhancers. 
B)  support the grouping of microbes based on different characteristics demonstrated in the media. 
C)  support the growth of most nonfastidious microbes. 
D)  support the growth of one type or group of microbes, but not another.

Exam 6: Specimen Collection and Processing

Nonselective media supports

What work practice have microbiologists incorporated to keep laboratory testing cost-effective while providing optimum patient care?

The requisition form should provide all the following information, except

All the following principles of specimen collection are fundamental to ensuring appropriate specimen management, except:

The purpose of transport media is to

To assist hospital personnel in collecting the highest quality specimen, the laboratory should

Sputum specimens are often collected for the diagnosis of

When using the isolation streaking technique, the most isolated forms of the organism should be in which quadrant?

Data generated by the laboratory is directly influenced by

How should specimen collection instructions be given to the patient to ensure collection of a good specimen for culture?

Proper identification of each specimen includes a label firmly attached to the container with all the following information, except

What is the primary goal in the transportation of specimens to the laboratory?

Routine primary plating media include all the following items, except

Notations from the macroscopic (gross) examination of a specimen should include all the following, except

Specimens such as blood, bone marrow, and synovium are mixed with anticoagulants right after collection. Why should this occur?

Why is clean-catch midstream urine used for a urine culture as opposed to a clean-catch urine?

Types of specimens that can be batch processed, including all the following, except

Most bacterial cultures are incubated at this temperature range.

Selective media

Bacterial Cell Structure, Physiology, Metabolism, and Genetics

Hostparasite Interaction

The Laboratory Role in Infection Control

Control of Microorganisms

Performance Improvement in the Microbiology Laboratory

Microscopic Examination of Materials From Infected Sites

Use of Colony Morphology for the Presumptive Identification of Microorganisms

Biochemical Identification of Gram-Negative Bacteria

Immunodiagnosis of Infectious Diseases

Applications of Molecular Diagnostics

Antimicrobial Agent Mechanisms of Action and Resistance Mechanisms

Antimicrobial Susceptibility Testing

Staphylococci

Streptococcus, Enterococcus, and Other Catalase-Negative, Gram-Positive Cocci

Aerobic Gram-Positive Bacilli

Neisseria Species and Moraxella Catarrhalis

Haemophilus, Hacek, Legionella, and Other Fastidious Gram-Negative Bacilli

Enterobacteriaceae

Vibrio, Aeromonas, and Campylobacter Species

Nonfermenting and Miscellaneous Gram-Negative Bacilli

Anaerobes of Clinical Importance

The Spirochetes

Chlamydia, Rickettsia, and Similar Organisms

Mycoplasma and Ureaplasma

Mycobacterium Tuberculosis and Nontuberculous Mycobacteria

Medically Significant Fungi

Diagnostic Parasitology

Clinical Virology

Agents of Bioterror and Forensic Microbiology

Biofilms: Architects of Disease

Upper and Lower Respiratory Tract Infections

Skin and Soft Tissue Infections

Gastrointestinal Infections and Food Poisoning

Infections of the Central Nervous System

Bacteremia and Sepsis

Urinary Tract Infections

Genital Infections and Sexually Transmitted Infections

Infections in Special Populations

Zoonotic Diseases

Ocular Infections

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