Question 1

Describe two processes for which recombination is essential.

Accepted Answer

The exchange of genetic information during meiosis,and the repair of double- stranded breaks in DNA molecules.

Question 2

Assembly of new nucleosomes is carried out by complexes abbreviated_________ .

Accepted Answer

A) DSB 
B) TLS 
C) TERC 
D) SSB 
E) CAF 
A) DSB 
B) TLS 
C) TERC 
D) SSB 
E) CAF E

Question 3

What is the name of the replication unit in prokaryotes,and how does it differ in eukaryotes?

Accepted Answer

replicon; one replicon in prokaryotes,multiple replicons in eukaryotes

Question 4

Telomerase adds nucleotides to the_________ .

Accepted Answer

A) 5' end of the daughter strand 
B) 5' end of the parental strand 
C) 3' end of the parental strand 
D) 3' end of the daughter strand 
A) 5' end of the daughter strand 
B) 5' end of the parental strand 
C) 3' end of the parental strand 
D) 3' end of the daughter strand

Question 5

Which eukaryotic DNA polymerase would least likely be involved in repair?

Accepted Answer

A) fi 
B) s 
C) a 
D) 6 
E) y 
A) fi 
B) s 
C) a 
D) 6 
E) y

Question 6

The process of making DNA from an RNA template is referred to as_________ .

Accepted Answer

A) transcription 
B) transversion 
C) recombination 
D) reverse transcription 
E) translation 
A) transcription 
B) transversion 
C) recombination 
D) reverse transcription 
E) translation

Question 7

Which of the following statement about DNA replication in E.coli is correct?

Accepted Answer

A) Single- stranded binding proteins are only required on the leading strand. 
B) Only the lagging strand needs to be primed. 
C) There are two molecules of DNA polymerase III per replication fork. 
D) DNA polymerase I follows closely behind DNA helicase in the replication fork. 
E) There are two molecules of DNA helicase per replication fork. 
A) Single- stranded binding proteins are only required on the leading strand. 
B) Only the lagging strand needs to be primed. 
C) There are two molecules of DNA polymerase III per replication fork. 
D) DNA polymerase I follows closely behind DNA helicase in the replication fork. 
E) There are two molecules of DNA helicase per replication fork.

Question 8

Telomerase reads _________ as a template and synthesizes _________as a product.

Accepted Answer

A) DNA; RNA 
B) DNA; DNA 
C) DNA; protein 
D) RNA; RNA 
E) RNA; DNA 
A) DNA; RNA 
B) DNA; DNA 
C) DNA; protein 
D) RNA; RNA 
E) RNA; DNA

Question 9

After ONE round of DNA replication in Meselson and Stahl's classic experiment,_________.

Accepted Answer

A) the conservative model was conclusively proven to be an inaccurate model 
B) both the conservative and the semiconservative models were still possibilities 
C) the semiconservative model was conclusively proven to be the accurate model 
D) the dispersive model was conclusively proven to be an inaccurate model 
E) all three models of replication were still possibilities 
A) the conservative model was conclusively proven to be an inaccurate model 
B) both the conservative and the semiconservative models were still possibilities 
C) the semiconservative model was conclusively proven to be the accurate model 
D) the dispersive model was conclusively proven to be an inaccurate model 
E) all three models of replication were still possibilities

Question 10

Explain the need for,and action of,DNA gyrase in bacterial DNA replication.

Accepted Answer

DNA helicase induces supercoil

Question 11

Given the accompanying diagram,assume that a G1 chromosome left underwent one round of replication in 3H- thymidine and the metaphase chromosome right)had both chromatids labeled.Which of the following replicative models conservative,dispersive,semiconservative)could be eliminated by this observation?

Accepted Answer

A) semiconservative 
B) dispersive 
C) conservative 
D) conservative and dispersive 
E) semiconservative and dispersive 
A) semiconservative 
B) dispersive 
C) conservative 
D) conservative and dispersive 
E) semiconservative and dispersive

Question 12

Chromatin is defined as_________ .

Accepted Answer

A) DNA complexed with protein 
B) supercoiled loops of bacterial DNA 
C) hybrid molecules of DNA and RNA 
D) a catalytic molecule with a protein component and an RNA component 
E) genetic material found only in mitochondria 
A) DNA complexed with protein 
B) supercoiled loops of bacterial DNA 
C) hybrid molecules of DNA and RNA 
D) a catalytic molecule with a protein component and an RNA component 
E) genetic material found only in mitochondria

Question 13

The Meselson and Stahl's experiment provided conclusive evidence for the semiconservative replication of DNA in E.coli.What pattern of bands would occur in a CsCl gradient for conservative replication?

Accepted Answer

After one generation in the 14N,there wo

Question 14

DNA polymerase III adds nucleotides_________ .

Accepted Answer

A) in the place of the primer RNA after it is removed 
B) to internal sites in the DNA template 
C) to the 5' end of the RNA primer 
D) to the 3' end of the RNA primer 
E) to both ends of the RNA primer 
A) in the place of the primer RNA after it is removed 
B) to internal sites in the DNA template 
C) to the 5' end of the RNA primer 
D) to the 3' end of the RNA primer 
E) to both ends of the RNA primer

Question 15

What is meant by a gene knockout?

Accepted Answer

The designed loss of a specific gene in

Question 16

DNA replication in vivo requires a primer with a free 3' end.What molecular species provides this 3' end,and how is it provided?

Accepted Answer

The free 3' end is provided by

Question 17

A molecule that possesses both a protein component and an RNA component is referred to as a _________.

Accepted Answer

A) holoenzyme 
B) ribonucleoprotein 
C) polymerase 
D) tetramer 
E) dimer 
A) holoenzyme 
B) ribonucleoprotein 
C) polymerase 
D) tetramer 
E) dimer

Question 18

Explain why DNA primers can be used in in vitro DNA synthesis reactions,but not in vivo.

Accepted Answer

The need for the primer is to provide a

Question 19

Describe the benefit to researchers of conditional mutants.

Accepted Answer

Many mutations that would be interesting

Question 20

Why are bacteria not dependent on telomerase for complete DNA replication?

Accepted Answer

Telomerase allows for the accu

Exam 11: DNA Replication and Recombination

Describe two processes for which recombination is essential.

Assembly of new nucleosomes is carried out by complexes abbreviated_________ .

What is the name of the replication unit in prokaryotes,and how does it differ in eukaryotes?

Telomerase adds nucleotides to the_________ .

Which eukaryotic DNA polymerase would least likely be involved in repair?

The process of making DNA from an RNA template is referred to as_________ .

Which of the following statement about DNA replication in E.coli is correct?

Telomerase reads _________ as a template and synthesizes _________as a product.

After ONE round of DNA replication in Meselson and Stahl's classic experiment,_________.

Explain the need for,and action of,DNA gyrase in bacterial DNA replication.

Chromatin is defined as_________ .

The Meselson and Stahl's experiment provided conclusive evidence for the semiconservative replication of DNA in E.coli.What pattern of bands would occur in a CsCl gradient for conservative replication?

DNA polymerase III adds nucleotides_________ .

What is meant by a gene knockout?

DNA replication in vivo requires a primer with a free 3' end.What molecular species provides this 3' end,and how is it provided?

A molecule that possesses both a protein component and an RNA component is referred to as a _________.

Explain why DNA primers can be used in in vitro DNA synthesis reactions,but not in vivo.

Describe the benefit to researchers of conditional mutants.

Why are bacteria not dependent on telomerase for complete DNA replication?

Introduction to Genetics

Mitosis and Meiosis

Mendelian Genetics

Extensions of Mendelian Genetics

Chromosome Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophages

Sex Determination and Sex Chromosomes

Chromosome Mutations: Variation in Number and Arrangement

Extranuclear Inheritance

DNA Structure and Analysis

DNA Organization in Chromosomes

The Genetic Code and Transcription

Translation and Proteins

Gene Mutation,dna Repair,and Transposition

Regulation of Gene Expression in Bacteria

Transcriptional Regulation in Eukaryotes

Post-Transcriptional Regulation in Eukaryotes

Epigenetic Regulation in Eukaryotes

Recombinant Dna Technology

Genomic Analysis

Applications of Genetic Engineering and Biotechnology

Developmental Genetics

Cancer Genetics

Quantitative Genetics and Multifactorial Traits

Population and Evolutionary Genetics

CRISPR and Genomic Editing

DNA Forensics

Genomics and Precision Medicine

Genetically Modified Foods

Gene Therapy

Advances in Neurogenetics: The Study of Huntington Disease

Filters

Telomerase reads _ as a template and synthesizes _as a product.