Question 1

Compare and contrast DNA replication in prokaryotes and eukaryotes.

Accepted Answer

Eukaryotic DNA is replicated in a manner

Question 2

A drug is added to a culture of E.coli cells that inhibit DNA gyrase.Predict the effect of this drug on DNA replication in the affected cells.

Accepted Answer

Without DNA gyrase to relieve the superc

Question 3

Which DNA Polymerase III subunit is not part of the clamp loading assembly?

Accepted Answer

A) 0 
B) ç 
C) 6 
D) v 
E) y 
A) 0 
B) ç 
C) 6 
D) v 
E) y

Question 4

Briefly describe what is meant by the term autoradiography and identify a classic experiment that used autoradiography to determine the replicative nature of DNA in eukaryotes.

Accepted Answer

Autoradiography is a technique

Question 5

What is meant by homologous recombination?

Accepted Answer

Genetic exchange at equivalent

Question 6

DNA polymerase I is thought to add nucleotides_________ .

Accepted Answer

A) to the 5' end of the primer 
B) on single- stranded templates without need for an RNA primer 
C) to the 3' end of the primer 
D) in the place of the primer RNA after it is removed 
E) in a 5' to 5' direction 
A) to the 5' end of the primer 
B) on single- stranded templates without need for an RNA primer 
C) to the 3' end of the primer 
D) in the place of the primer RNA after it is removed 
E) in a 5' to 5' direction

Question 7

As unwinding of the helix occurs during DNA replication,tension referred to as_________ is created ahead of the replication fork.This tension is relieved by the action of_________ .

Accepted Answer

A) supercoiling; single- stranded binding proteins 
B) processivity; DNA gyrase 
C) supercoiling; DNA gyrase 
D) supercoiling; DNA helicase 
E) processivity; primase 
A) supercoiling; single- stranded binding proteins 
B) processivity; DNA gyrase 
C) supercoiling; DNA gyrase 
D) supercoiling; DNA helicase 
E) processivity; primase

Question 8

Which cluster of terms accurately reflects the nature of DNA replication in prokaryotes?

Accepted Answer

A) fixed point of initiation,bidirectional,conservative 
B) random point of initiation,bidirectional,semiconservative 
C) random point of initiation,unidirectional,semiconservative 
D) fixed point of initiation,bidirectional,semiconservative 
E) fixed point of initiation,unidirectional,conservative 
A) fixed point of initiation,bidirectional,conservative 
B) random point of initiation,bidirectional,semiconservative 
C) random point of initiation,unidirectional,semiconservative 
D) fixed point of initiation,bidirectional,semiconservative 
E) fixed point of initiation,unidirectional,conservative

Question 9

Describe a somewhat extraordinary finding related to the Tetrahymena telomerase enzyme.

Accepted Answer

The enzyme contains

Question 10

DNA replication proceeds_________ .

Accepted Answer

A) discontinuously 
B) progressively 
C) semiconservatively 
D) dispersively 
E) unidirectionally 
A) discontinuously 
B) progressively 
C) semiconservatively 
D) dispersively 
E) unidirectionally

Question 11

Describe the DNA base sequence arrangement at the end of the Tetrahymena chromosome and the resolution of DNA replication at the end of a linear DNA strand.

Accepted Answer

Telomeres terminate in a 5'- TTGGGG- 3'

Question 12

Which terms accurately reflect the nature of replication of the chromosome in E.coli?

Accepted Answer

A) bidirectional and multirepliconic 
B) bidirectional and fixed point of initiation 
C) unidirectional and fixed point of initiation 
D) multirepliconic and telomeric 
E) unidirectional and reciprocal 
A) bidirectional and multirepliconic 
B) bidirectional and fixed point of initiation 
C) unidirectional and fixed point of initiation 
D) multirepliconic and telomeric 
E) unidirectional and reciprocal

Question 13

Which of the following statements about the oriC region in E.coli is incorrect?

Accepted Answer

A) It includes five repeating sequences of 9 base pairs. 
B) It is 245 base pairs in length. 
C) It includes three repeating sequences of 13 base pairs. 
D) It is rich in AT base pairs. 
E) It is directly recognized and bound by DNA helicase. 
A) It includes five repeating sequences of 9 base pairs. 
B) It is 245 base pairs in length. 
C) It includes three repeating sequences of 13 base pairs. 
D) It is rich in AT base pairs. 
E) It is directly recognized and bound by DNA helicase.

Question 14

The amount of DNA that is replicated from a single origin of replication is referred to as an_________ .

Accepted Answer

A) Okazaki fragment 
B) ori 
C) fork 
D) primer 
E) replicon 
A) Okazaki fragment 
B) ori 
C) fork 
D) primer 
E) replicon

Question 15

Which of the following is not an essential step in homologous recombination?

Accepted Answer

A) exonuclease degradation 
B) strand displacement 
C) duplex separation 
D) branch migration 
E) endonuclease nicking 
A) exonuclease degradation 
B) strand displacement 
C) duplex separation 
D) branch migration 
E) endonuclease nicking

Question 16

Why does DNA replication have to be semidiscontinuous in order to be bidirectional?

Accepted Answer

The requirement of synthesis to be 5' to

Question 17

DNA replication in eukaryotes_________ .

Accepted Answer

A) occurs without the need of a primer 
B) takes place multiple times per cell cycle 
C) synthesizes DNA approximately 25 times faster than in prokaryotes 
D) utilizes a single type of DNA polymerase 
E) initiates at multiple origins 
A) occurs without the need of a primer 
B) takes place multiple times per cell cycle 
C) synthesizes DNA approximately 25 times faster than in prokaryotes 
D) utilizes a single type of DNA polymerase 
E) initiates at multiple origins

Question 18

DNA replication occurs by adding_________ .

Accepted Answer

A) NTPs to the 5' end of the daughter strand 
B) dNTPs to the 3' end of the template strand 
C) NTPs to the 3' end of the daughter strand 
D) dNTPs to the 3' end of the daughter strand 
E) dNTPs to the 5' end of the template strand 
A) NTPs to the 5' end of the daughter strand 
B) dNTPs to the 3' end of the template strand 
C) NTPs to the 3' end of the daughter strand 
D) dNTPs to the 3' end of the daughter strand 
E) dNTPs to the 5' end of the template strand

Question 19

If E.coli DNA polymerase III was able to initiate DNA synthesis opposite a single- stranded template,which molecules)would no longer be essential?

Accepted Answer

A) single- stranded binding proteins 
B) DNA gyrase 
C) DNA ligase 
D) DNA polymerase I 
E) helicase 
A) single- stranded binding proteins 
B) DNA gyrase 
C) DNA ligase 
D) DNA polymerase I 
E) helicase

Question 20

Compare and contrast DNA Polymerases I,II,and III in E.coli.What capabilities are shared,and which are exclusive to only one type of polymerase?

Accepted Answer

None of the polymerases are able to init

Exam 11: DNA Replication and Recombination

Compare and contrast DNA replication in prokaryotes and eukaryotes.

A drug is added to a culture of E.coli cells that inhibit DNA gyrase.Predict the effect of this drug on DNA replication in the affected cells.

Which DNA Polymerase III subunit is not part of the clamp loading assembly?

Briefly describe what is meant by the term autoradiography and identify a classic experiment that used autoradiography to determine the replicative nature of DNA in eukaryotes.

What is meant by homologous recombination?

DNA polymerase I is thought to add nucleotides_________ .

As unwinding of the helix occurs during DNA replication,tension referred to as_________ is created ahead of the replication fork.This tension is relieved by the action of_________ .

Which cluster of terms accurately reflects the nature of DNA replication in prokaryotes?

Describe a somewhat extraordinary finding related to the Tetrahymena telomerase enzyme.

DNA replication proceeds_________ .

Describe the DNA base sequence arrangement at the end of the Tetrahymena chromosome and the resolution of DNA replication at the end of a linear DNA strand.

Which terms accurately reflect the nature of replication of the chromosome in E.coli?

Which of the following statements about the oriC region in E.coli is incorrect?

The amount of DNA that is replicated from a single origin of replication is referred to as an_________ .

Which of the following is not an essential step in homologous recombination?

Why does DNA replication have to be semidiscontinuous in order to be bidirectional?

DNA replication in eukaryotes_________ .

DNA replication occurs by adding_________ .

If E.coli DNA polymerase III was able to initiate DNA synthesis opposite a single- stranded template,which molecules)would no longer be essential?

Compare and contrast DNA Polymerases I,II,and III in E.coli.What capabilities are shared,and which are exclusive to only one type of polymerase?

Introduction to Genetics

Mitosis and Meiosis

Mendelian Genetics

Extensions of Mendelian Genetics

Chromosome Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophages

Sex Determination and Sex Chromosomes

Chromosome Mutations: Variation in Number and Arrangement

Extranuclear Inheritance

DNA Structure and Analysis

DNA Organization in Chromosomes

The Genetic Code and Transcription

Translation and Proteins

Gene Mutation,dna Repair,and Transposition

Regulation of Gene Expression in Bacteria

Transcriptional Regulation in Eukaryotes

Post-Transcriptional Regulation in Eukaryotes

Epigenetic Regulation in Eukaryotes

Recombinant Dna Technology

Genomic Analysis

Applications of Genetic Engineering and Biotechnology

Developmental Genetics

Cancer Genetics

Quantitative Genetics and Multifactorial Traits

Population and Evolutionary Genetics

CRISPR and Genomic Editing

DNA Forensics

Genomics and Precision Medicine

Genetically Modified Foods

Gene Therapy

Advances in Neurogenetics: The Study of Huntington Disease

Filters

As unwinding of the helix occurs during DNA replication,tension referred to as_ is created ahead of the replication fork.This tension is relieved by the action of_ .