Question 1

Which of the following represents the degree of light scatter that reflects cell complexity on the CELL-DYN Sapphire?

Accepted Answer

A)  0 degrees 
B)  7 degrees 
C)  90 degrees 
D)  90 degrees depolarized 
A)  0 degrees 
B)  7 degrees 
C)  90 degrees 
D)  90 degrees depolarized B

Question 2

Which instrument does not use fluorescence intensity in its determination of reticulocytes?

Accepted Answer

A)  LH 750 
B)  XE-2100 
C)  ADVIA 120 
D)  CELL-DYN Sapphire 
A)  LH 750 
B)  XE-2100 
C)  ADVIA 120 
D)  CELL-DYN Sapphire A

Question 3

Which instrument utilizes contour gating to classify different leukocyte cell types?

Accepted Answer

A)  ADVIA 120 
B)  XE-2100 
C)  LH 750 
D)  CELL-DYN Sapphire 
A)  ADVIA 120 
B)  XE-2100 
C)  LH 750 
D)  CELL-DYN Sapphire C

Question 4

Which parameter does the Sysmex XE-2100 instrument determine by using light-scattering and fluorescence intensity characteristics?

Accepted Answer

A)  Platelet count 
B)  Erythrocyte count 
C)  Absolute basophil count 
D)  Absolute lymphocyte count 
A)  Platelet count 
B)  Erythrocyte count 
C)  Absolute basophil count 
D)  Absolute lymphocyte count

Question 5

The Sysmex XE-2100 instrument printout reports a middle fluorescent ratio of 7.0 and a high fluorescent ratio of 1.3. What is the immature reticulocyte fraction?

Accepted Answer

A)  9.1 
B)  91.7 
C)  8.3 
D)  5.7 
A)  9.1 
B)  91.7 
C)  8.3 
D)  5.7

Question 6

Which of the following binds to residual RNA in reticulocytes on the Sysmex XE-2100 analyzer?

Accepted Answer

A)  New methylene blue 
B)  Propidium iodide 
C)  Polymethine 
D)  Oxazine 
A)  New methylene blue 
B)  Propidium iodide 
C)  Polymethine 
D)  Oxazine

Question 7

Compare and contrast the methods of detection for the following analyzers:
a. Sysmex XE-5000
b. Abbott Cell-Dyn Sapphire
c. Coulter DxH 800
d. Advia 2120

Accepted Answer

The answer of Compare and contrast the methods of detection...

Question 8

The immature platelet fraction (IPF) is a parameter derived from the Sysmex XE-2100's RET scattergram. An increased IPF is associated with:

Accepted Answer

A)  Aplastic anemia 
B)  Pernicious anemia 
C)  Acute lymphocytic leukemia 
D)  Immune thrombocytopenic purpura 
A)  Aplastic anemia 
B)  Pernicious anemia 
C)  Acute lymphocytic leukemia 
D)  Immune thrombocytopenic purpura

Question 9

In the reticulocyte determination, using the Sysmex XE-2100, the fluorescent dye containing oxazine and polymethine penetrates the cell membranes and stains the blood cells. What does the oxazine bind to within the cells?

Accepted Answer

A)  Residual DNA 
B)  Hemoglobin 
C)  Residual RNA 
D)  Mitochondria 
A)  Residual DNA 
B)  Hemoglobin 
C)  Residual RNA 
D)  Mitochondria

Question 10

What is the principle of impedance? Name at least two instruments that employ this method for blood cell counting.

Accepted Answer

Impedance relies on the principle of inc

Question 11

The principle that relies on detecting resistance as cells pass through a photo-detector and is used by most of the major hematology analyzers is:

Accepted Answer

A)  Spectrophotometry 
B)  Impedance 
C)  Radio frequency 
D)  MAPSS technology 
A)  Spectrophotometry 
B)  Impedance 
C)  Radio frequency 
D)  MAPSS technology

Question 12

Regarding the CELL-DYN Sapphire, which parameter is determined by the impedance principle?

Accepted Answer

A)  Hemoglobin 
B)  Nucleated erythrocyte count 
C)  Platelet count 
D)  Total leukocyte count 
A)  Hemoglobin 
B)  Nucleated erythrocyte count 
C)  Platelet count 
D)  Total leukocyte count

Question 13

When using the CELL-DYN Sapphire to perform CBCs, a laboratory professional can choose to run the ImmunoPlt assay if:

Accepted Answer

A)  Leukocyte count is decreased 
B)  Erythrocyte fragments are detected 
C)  Nucleated erythrocytes are observed 
D)  Erythrocyte count is increased 
A)  Leukocyte count is decreased 
B)  Erythrocyte fragments are detected 
C)  Nucleated erythrocytes are observed 
D)  Erythrocyte count is increased

Question 14

Which analyzer has the ability to quantify nucleated RBCs and calculate a corrected WBC count?

Accepted Answer

A)  Coulter Gen-S 
B)  Sysmex XE-2100 
C)  Abbott Cell Dyn Sapphire 
D)  Coulter LH 780 
A)  Coulter Gen-S 
B)  Sysmex XE-2100 
C)  Abbott Cell Dyn Sapphire 
D)  Coulter LH 780

Question 15

Which analyzer does not use cyanide-free detection of hemoglobin?

Accepted Answer

A)  Advia 120 
B)  Advia 2120 
C)  Abbott Cell-Dyn Sapphire 
D)  Sysmex XE-2100 
A)  Advia 120 
B)  Advia 2120 
C)  Abbott Cell-Dyn Sapphire 
D)  Sysmex XE-2100

Question 16

Which of the following is a reported parameter for the ADVIA 120 but not for the LH 750 instrument?

Accepted Answer

A)  RDW-CV 
B)  Retic% 
C)  CHCM 
D)  MCVr 
A)  RDW-CV 
B)  Retic% 
C)  CHCM 
D)  MCVr

Question 17

Which technologies are used to generate the LH 750's WBC scatterplot?

Accepted Answer

A)  Impedance, light scatter, and conductivity 
B)  Fluorescence intensity and radio frequency 
C)  Absorbance and conductivity 
D)  Light scatter, absorbance, and radio frequency 
A)  Impedance, light scatter, and conductivity 
B)  Fluorescence intensity and radio frequency 
C)  Absorbance and conductivity 
D)  Light scatter, absorbance, and radio frequency

Question 18

Explain the purpose of the following used in the standard hematologic cell-counting detection methods:
a. VCS flow cell
b. Photodetector
c. Sheath fluid
d. Direct current detection block

Accepted Answer

a. VCS flow cell: to detect each WBC's v

Question 19

Cell volume and cell number are required elements for which of the following?

Accepted Answer

A)  WBC scatterplots 
B)  WBC cytograms 
C)  RBC histograms 
D)  RBC scattergrams 
A)  WBC scatterplots 
B)  WBC cytograms 
C)  RBC histograms 
D)  RBC scattergrams

Question 20

Hydrodynamic focusing is used in automated cell-counting instruments to:

Accepted Answer

A)  Prevent coincidence passage through the detection aperture 
B)  Increase the visibility of the cells passing through the aperture 
C)  Allow for maximal light absorbance through the cells passing through the aperture 
D)  Ensure adequate uptake of fluorescent staining by the cells 
A)  Prevent coincidence passage through the detection aperture 
B)  Increase the visibility of the cells passing through the aperture 
C)  Allow for maximal light absorbance through the cells passing through the aperture 
D)  Ensure adequate uptake of fluorescent staining by the cells

Exam 39: Automation in Hematology

Which of the following represents the degree of light scatter that reflects cell complexity on the CELL-DYN Sapphire?

Which instrument does not use fluorescence intensity in its determination of reticulocytes?

Which instrument utilizes contour gating to classify different leukocyte cell types?

Which parameter does the Sysmex XE-2100 instrument determine by using light-scattering and fluorescence intensity characteristics?

The Sysmex XE-2100 instrument printout reports a middle fluorescent ratio of 7.0 and a high fluorescent ratio of 1.3. What is the immature reticulocyte fraction?

Which of the following binds to residual RNA in reticulocytes on the Sysmex XE-2100 analyzer?

Compare and contrast the methods of detection for the following analyzers: a. Sysmex XE-5000 b. Abbott Cell-Dyn Sapphire c. Coulter DxH 800 d. Advia 2120

The immature platelet fraction (IPF) is a parameter derived from the Sysmex XE-2100's RET scattergram. An increased IPF is associated with:

In the reticulocyte determination, using the Sysmex XE-2100, the fluorescent dye containing oxazine and polymethine penetrates the cell membranes and stains the blood cells. What does the oxazine bind to within the cells?

What is the principle of impedance? Name at least two instruments that employ this method for blood cell counting.

The principle that relies on detecting resistance as cells pass through a photo-detector and is used by most of the major hematology analyzers is:

Regarding the CELL-DYN Sapphire, which parameter is determined by the impedance principle?

When using the CELL-DYN Sapphire to perform CBCs, a laboratory professional can choose to run the ImmunoPlt assay if:

Which analyzer has the ability to quantify nucleated RBCs and calculate a corrected WBC count?

Which analyzer does not use cyanide-free detection of hemoglobin?

Which of the following is a reported parameter for the ADVIA 120 but not for the LH 750 instrument?

Which technologies are used to generate the LH 750's WBC scatterplot?

Explain the purpose of the following used in the standard hematologic cell-counting detection methods: a. VCS flow cell b. Photodetector c. Sheath fluid d. Direct current detection block

Cell volume and cell number are required elements for which of the following?

Hydrodynamic focusing is used in automated cell-counting instruments to:

Introduction

Cellular Homeostasis

Structure and Function of Hematopoietic Organs

Hematopoiesis

The Erythrocyte

Hemoglobin

Granulocytes and Monocytes

Lymphocytes

The Platelet

The Complete Blood Count and Peripheral Blood Smear Evaluation

Introduction to Anemia

Anemias of Disordered Iron Metabolism and Heme Synthesis

Hemoglobinopathies: Qualitative Defects

Thalassemia

Megaloblastic and Nonmegaloblastic Macrocytic Anemias

Hypoproliferative Anemias

Hemolytic Anemia: Membrane Defects

Hemolytic Anemia: Enzyme Deficiencies

Hemolytic Anemia: Immune Anemias

Hemolytic Anemia: Nonimmune Defects

Nonmalignant Disorders of Leukocytes: Granulocytes and Monocytes

Nonmalignant Lymphocyte Disorders

Introduction to Hematopoietic Neoplasms

Myeloproliferative Neoplasms

Myelodysplastic Syndromes

Acute Myeloid Leukemias

Precursor Lymphoid Neoplasms

Mature Lymphoid Neoplasms

Hematopoietic Stem Cell Transplantation

Morphologic Analysis of Body Fluids in the Hematology

Primary Hemostasis

Secondary Hemostasis and Fibrinolysis

Disorders of Primary Hemostasis

Disorders of Secondary Hemostasis

Thrombophilia

Hemostasis: Laboratory Testing and Instrumentation

Hematology Procedures

Bone Marrow Examination

Flow Cytometry

Chromosome Analysis of Hematopoietic and Lymphoid Disorders

Molecular Analysis of Hematologic Diseases

Quality Assessment in the Hematology Laboratory

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