Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

_____ is the term for a starting a new culture from an existing one.

The three physical forms of laboratory media are: solid, semisolid, and _____.

Microbes can be challenging to study because they often grow in complex communities and it may be difficult to separate the various species from one another to study individually. Which of the following laboratory "tools" or techniques may be of the the most help with separating out different species from complex mixtures?

_____ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the slide to the objective lens.

A student was given a stained smear to observe. Upon observation, the student noted rod-shaped bacteria that were clear and unstained against a darker background. What sort of stain was performed on this slide?

The Six "I's" of studying microorganisms include all of the following except

Bacteria that require special growth factors and complex organic substances are called

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is

You are doing research on a bacterial species, trying to determine the shape, distribution, and arrangement of a number of filamentous structures that protrude from the cell surface. Which type of microscopy would best enable you to do this?

Syphilis is diagnosed either with microscopy or serology because the causative agent Treponema pallidum) does not grow on any microbiological media. Which term best describes this aspect of the T. pallidum?

One colony typically develops from the growth of several parent bacterial cells.

Which microscope does not use light in forming the specimen image?

Which method often results in colonies developing down throughout the agar and some colonies on the surface?

At the end of the Gram stain, gram-positive bacteria will be seen as purple cells.

Media that contains extracts from plants, animals, or yeasts are

To prepare large volumes of influenza virus for use in the flu vaccines each year, the virus is grown in embryonated hen eggs. This is an example of the use of

Each of the following are characteristics of microorganisms that pose unique challenges to their study EXCEPT

All of the following are examples of basic dyes except

Which microscope shows cells against a bright background and the intracellular structures of unstained cells based on their varying densities?

Which of the following media is useful for cultivating fungi?