Question 1

All of the following pertain to the fluorescence microscope except it

Accepted Answer

A)  uses electrons to produce a specimen image. 
B)  is a type of compound microscope. 
C)  requires the use of dyes like acridine and fluorescein. 
D)  is commonly used to diagnose certain infections. 
E)  requires an ultraviolet radiation source. 
A)  uses electrons to produce a specimen image. 
B)  is a type of compound microscope. 
C)  requires the use of dyes like acridine and fluorescein. 
D)  is commonly used to diagnose certain infections. 
E)  requires an ultraviolet radiation source.

Question 2

All of the following are correct about agar except

Accepted Answer

A)  it is flexible. 
B)  it melts at the boiling point of water 100°
C) . 
C)  it is a source of nutrition for bacteria. 
D)  it solidifies below 42°C. 
E)  it is solid at room temperature. 
A)  it is flexible. 
B)  it melts at the boiling point of water 100°
C) . 
C)  it is a source of nutrition for bacteria. 
D)  it solidifies below 42°C. 
E)  it is solid at room temperature.

Question 3

The specimen preparation that is best for viewing cell motility is

Accepted Answer

A)  hanging drop. 
B)  fixed-stained smear. 
C)  Gram stain. 
D)  negative stain. 
E)  flagellar stain. 
A)  hanging drop. 
B)  fixed-stained smear. 
C)  Gram stain. 
D)  negative stain. 
E)  flagellar stain.

Question 4

You are doing research on a bacterial species, trying to determine the nature and structure of a number of intracellular inclusions. Which type of microscopy would provide the best view of these intracellular structures?

Accepted Answer

A)  scanning electron microscopy 
B)  transmission electron microscopy 
C)  bright-field microscopy 
D)  dark-Field microscopy 
A)  scanning electron microscopy 
B)  transmission electron microscopy 
C)  bright-field microscopy 
D)  dark-Field microscopy

Question 5

A microbiologist must culture a patient's feces for intestinal pathogens. Which of the following would likely be present in selective media for analyzing this fecal specimen?

Accepted Answer

A)  NaCl 
B)  sheep red blood cells 
C)  bile salts 
D)  thioglycolic acid 
E)  peptone 
A)  NaCl 
B)  sheep red blood cells 
C)  bile salts 
D)  thioglycolic acid 
E)  peptone

Question 6

A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue. All the cells appear blue under the oil lens. This is an example of

Accepted Answer

A)  negative staining. 
B)  using an acidic dye. 
C)  simple staining. 
D)  using the acid-fast stain. 
E)  capsule staining. 
A)  negative staining. 
B)  using an acidic dye. 
C)  simple staining. 
D)  using the acid-fast stain. 
E)  capsule staining.

Question 7

Scanning tunneling and atomic force microscopes are used to image the detailed structure of biological molecules.

Accepted Answer

A) True 
 B)False

Question 8

Why is immersion oil often used when viewing specimens under the microscope?

Accepted Answer

A)  to stain the cells blue 
B)  to prevent the smear from drying out 
C)  to slow bacterial movement so you can see cells better 
D)  to increase the resolution 
E)  to reduce the amount of heat reaching the slide from the light source 
A)  to stain the cells blue 
B)  to prevent the smear from drying out 
C)  to slow bacterial movement so you can see cells better 
D)  to increase the resolution 
E)  to reduce the amount of heat reaching the slide from the light source

Question 9

_____ dyes have a negative charge on the chromophore and are repelled by bacterial cells.

Accepted Answer

The answer of _____ dyes have a negative charge on...

Question 10

Which microscope is the most widely used to show stained cells against a bright background?

Accepted Answer

A)  bright-field 
B)  dark-field 
C)  phase-contrast 
D)  fluorescence 
E)  electron 
A)  bright-field 
B)  dark-field 
C)  phase-contrast 
D)  fluorescence 
E)  electron

Question 11

All of the following are criteria that are used to classify different types of microbiological growth media EXCEPT

Accepted Answer

A)  physical state or consistency. 
B)  purpose or function. 
C)  chemical composition. 
D)  sensory characteristics color, smell). 
A)  physical state or consistency. 
B)  purpose or function. 
C)  chemical composition. 
D)  sensory characteristics color, smell).

Question 12

Identification relies entirely on biochemical test results.

Accepted Answer

A) True 
 B)False

Question 13

Which of the following is essential for development of discrete, isolated colonies?

Accepted Answer

A)  broth medium 
B)  differential medium 
C)  selective medium 
D)  solid medium 
E)  assay medium 
A)  broth medium 
B)  differential medium 
C)  selective medium 
D)  solid medium 
E)  assay medium

Question 14

In lab, Tom was given a mixed culture. His objective is to isolate single colonies. What should be used to accomplish this goal?

Accepted Answer

A)  sterile swab, loop dilution, TSB 
B)  hockey stick, spread plate technique, and 4 degree Celsius incubation temperature 
C)  loop dilution, TSA, hockey stick, 37 degree Celsius incubator 
D)  inoculating loop, incinerator, streak plate method, 37 degree Celsius incubator 
E)  sterile swab, streak plate method, 37 degree Celsius incubator 
A)  sterile swab, loop dilution, TSB 
B)  hockey stick, spread plate technique, and 4 degree Celsius incubation temperature 
C)  loop dilution, TSA, hockey stick, 37 degree Celsius incubator 
D)  inoculating loop, incinerator, streak plate method, 37 degree Celsius incubator 
E)  sterile swab, streak plate method, 37 degree Celsius incubator

Question 15

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens.

Accepted Answer

A) True 
 B)False

Question 16

How will

Accepted Answer

A)  gram-positive 
B)  gram-negative 
C)  gram-variable 
D)  colorless 
E)  coli appear if the mordant is not applied? 
A)  gram-positive 
B)  gram-negative 
C)  gram-variable 
D)  colorless 
E)  coli appear if the mordant is not applied?

Question 17

Which of the following reagents reacts with crystal violet as the mordant?

Accepted Answer

A)  crystal violet 
B)  Gram's iodine 
C)  95% ethyl alcohol 
D)  safranin 
A)  crystal violet 
B)  Gram's iodine 
C)  95% ethyl alcohol 
D)  safranin

Question 18

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible would be termed

Accepted Answer

A)  complex. 
B)  reducing. 
C)  enriched. 
D)  enumeration. 
E)  synthetic. 
A)  complex. 
B)  reducing. 
C)  enriched. 
D)  enumeration. 
E)  synthetic.

Question 19

A bacterial species that grows on blood agar but will not grow on trypticase soy agar is termed an anaerobe.

Accepted Answer

A) True 
 B)False

Question 20

The procedure for culturing a microorganism requires the use of a microscope.

Accepted Answer

A) True 
 B)False

Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

All of the following pertain to the fluorescence microscope except it

All of the following are correct about agar except

The specimen preparation that is best for viewing cell motility is

You are doing research on a bacterial species, trying to determine the nature and structure of a number of intracellular inclusions. Which type of microscopy would provide the best view of these intracellular structures?

A microbiologist must culture a patient's feces for intestinal pathogens. Which of the following would likely be present in selective media for analyzing this fecal specimen?

A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue. All the cells appear blue under the oil lens. This is an example of

Scanning tunneling and atomic force microscopes are used to image the detailed structure of biological molecules.

Why is immersion oil often used when viewing specimens under the microscope?

_____ dyes have a negative charge on the chromophore and are repelled by bacterial cells.

Which microscope is the most widely used to show stained cells against a bright background?

All of the following are criteria that are used to classify different types of microbiological growth media EXCEPT

Identification relies entirely on biochemical test results.

Which of the following is essential for development of discrete, isolated colonies?

In lab, Tom was given a mixed culture. His objective is to isolate single colonies. What should be used to accomplish this goal?

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens.

How will

Which of the following reagents reacts with crystal violet as the mordant?

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible would be termed

A bacterial species that grows on blood agar but will not grow on trypticase soy agar is termed an anaerobe.

The procedure for culturing a microorganism requires the use of a microscope.

The Main Themes of Microbiology

The Chemistry of Biology

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

Microbial Genetics

Genetic Engineering: a Revolution in Molecular Biology

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host--The Elements of Chemotherapy

Microbe-Human Interactions: Infection and Disease

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

Procedures for Identifying Pathogens and Diagnosing Infections

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

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