Question 1

Figure 9.2
  
-In Figure 9.2, the enzyme in step 2 is

Accepted Answer

A)  RNA polymerase. 
B)  DNA polymerase. 
C)  spliceosome. 
D)  reverse transcriptase. 
E)  DNA ligase. 
A)  RNA polymerase. 
B)  DNA polymerase. 
C)  spliceosome. 
D)  reverse transcriptase. 
E)  DNA ligase. D

Question 2

An advantage of cDNA over genomic DNA is that it

Accepted Answer

A)  can form very large DNA segments. 
B)  lacks exons. 
C) contains selectable markers. 
D) is very easy to isolate. 
E) lacks introns. 
A)  can form very large DNA segments. 
B)  lacks exons. 
C) contains selectable markers. 
D) is very easy to isolate. 
E) lacks introns. E

Question 3

Biotechnology involves the

Accepted Answer

A)  use of microorganisms to make desired products. 
B)  use of animal cells to make vaccines. 
C)  development of disease- resistant crop plants. 
D)  use of microorganisms to make desired products and the use of animal cells to make vaccines. 
E)  use of microorganisms to make desired products, the use of animal cells to make vaccines, and the development of disease- resistanct crop plants. 
A)  use of microorganisms to make desired products. 
B)  use of animal cells to make vaccines. 
C)  development of disease- resistant crop plants. 
D)  use of microorganisms to make desired products and the use of animal cells to make vaccines. 
E)  use of microorganisms to make desired products, the use of animal cells to make vaccines, and the development of disease- resistanct crop plants. E

Question 4

Assume you have discovered a cell that produces a lipase that works in cold water for a laundry additive. You can increase the efficiency of this enzyme by changing one amino acid. This is done by

Accepted Answer

A)  irradiating the cells. 
B)  site- directed mutagenesis. 
C)  selection. 
D)  selective breeding. 
E)  enrichment. 
A)  irradiating the cells. 
B)  site- directed mutagenesis. 
C)  selection. 
D)  selective breeding. 
E)  enrichment.

Question 5

The local public health agency has received reports of an outbreak of Salmonella gastroenteritis among attendees at a city- sponsored chili cook- off. What techniques from recombinant DNA technology would the agency likely use to investigate this outbreak? Describe the expected results from these techniques.

Accepted Answer

The local public health agency would lik

Question 6

Which of the following is NOT a desired characteristic of DNA vectors used in gene cloning procedures?

Accepted Answer

A)  has a selectable marker 
B)  self- replication 
C)  may replicate in several species 
D)  circular form of DNA or integrates into the host chromosome 
E)  large size 
A)  has a selectable marker 
B)  self- replication 
C)  may replicate in several species 
D)  circular form of DNA or integrates into the host chromosome 
E)  large size

Question 7

The study of genetic material taken directly from the environment is

Accepted Answer

A)  bioinformatics. 
B)  proteomics. 
C)  metagenomics. 
D)  forensic microbiology. 
E)  reverse genetics. 
A)  bioinformatics. 
B)  proteomics. 
C)  metagenomics. 
D)  forensic microbiology. 
E)  reverse genetics.

Question 8

The Ti plasmid isolated from Agrobacterium can be used to insert DNA into any type of plant.

Accepted Answer

A) True 
 B)False

Question 9

A restriction fragment is

Accepted Answer

A)  a segment of mRNA. 
B)  a segment of DNA. 
C)  cDNA. 
D)  a gene. 
E)  a segment of tRNA. 
A)  a segment of mRNA. 
B)  a segment of DNA. 
C)  cDNA. 
D)  a gene. 
E)  a segment of tRNA.

Question 10

Which of the following is NOT an agricultural product made by DNA techniques?

Accepted Answer

A)  pectinase 
B)  glyphosate- resistant crops 
C)  nitrogenase (nitrogen fixation) 
D)  Bacillus thuringiensis insecticide 
E)  frost retardant 
A)  pectinase 
B)  glyphosate- resistant crops 
C)  nitrogenase (nitrogen fixation) 
D)  Bacillus thuringiensis insecticide 
E)  frost retardant

Question 11

The Pap test for cervical cancer involves microscopic examination of cervical cells for cancerous cells. A new, rapid diagnostic test to detect human papilloma virus (HPV) DNA before cancer develops is done without microscopic exam. The steps involved in this FastHPV test are listed below. What is the second step?

Accepted Answer

A)  Add an RNA probe for HPV DNA. 
B)  Add enzyme- linked antibodies against DNA- RNA. 
C)  Add enzyme substrate. 
D)  Lyse human cells. 
E)  The order is unimportant. 
A)  Add an RNA probe for HPV DNA. 
B)  Add enzyme- linked antibodies against DNA- RNA. 
C)  Add enzyme substrate. 
D)  Lyse human cells. 
E)  The order is unimportant.

Question 12

An advantage of synthetic DNA over genomic or cDNA is the ability to

Accepted Answer

A)  obtain genes that lack introns. 
B)  insert desired restriction sites into the DNA sequence. 
C)  make DNA from cellular RNA and the enzyme reverse transcriptase. 
D)  isolate unknown genes. 
E)  obtain genes that lack exons. 
A)  obtain genes that lack introns. 
B)  insert desired restriction sites into the DNA sequence. 
C)  make DNA from cellular RNA and the enzyme reverse transcriptase. 
D)  isolate unknown genes. 
E)  obtain genes that lack exons.

Question 13

Which of the following places the steps in the PCR procedure in the correct order?
1) Incubate at 94°C to denature DNA strands;
2) Incubate at 72°C for DNA synthesis;
3) Incubate at 60°C for primer hybridization.

Accepted Answer

A)  1, 3, 2 
B)  1, 2, 3 
C)  3, 2, 1 
D)  3; 1; 2 
E)  2; 1; 3 
A)  1, 3, 2 
B)  1, 2, 3 
C)  3, 2, 1 
D)  3; 1; 2 
E)  2; 1; 3

Question 14

The random shotgun method is used in

Accepted Answer

A)  genome sequencing. 
B)  transforming plant cells with recombinant DNA. 
C)  RFLP analysis. 
D)  amplification of unknown DNA. 
E)  forensic microbiology. 
A)  genome sequencing. 
B)  transforming plant cells with recombinant DNA. 
C)  RFLP analysis. 
D)  amplification of unknown DNA. 
E)  forensic microbiology.

Question 15

The use of "suicide" genes in genetically modified organisms is designed to

Accepted Answer

A)  kill the modified organisms before they are released in the environment. 
B)  delete genes necessary for modified organism's growth. 
C)  provide a means to eliminate non- modified organisms. 
D)  provide for resistance of the modified organisms to pesticides. 
E)  prevent the growth of the modified organisms in the environment. 
A)  kill the modified organisms before they are released in the environment. 
B)  delete genes necessary for modified organism's growth. 
C)  provide a means to eliminate non- modified organisms. 
D)  provide for resistance of the modified organisms to pesticides. 
E)  prevent the growth of the modified organisms in the environment.

Question 16

Self- replicating DNA used to transmit a gene from one organism to another is a

Accepted Answer

A)  library. 
B)  vector. 
C)  clone. 
D)  Southern blot. 
E)  PCR. 
A)  library. 
B)  vector. 
C)  clone. 
D)  Southern blot. 
E)  PCR.

Question 17

In the Southern blot technique, which of the following is NOT required?

Accepted Answer

A)  restriction enzyme digestion of DNA 
B)  electrophoresis to separate fragments 
C)  addition of heat- stable DNA polymerase to amplify DNA 
D)  addition of a labeled probe to identify the gene of interest 
E)  transfer of DNA to nitrocellulose 
A)  restriction enzyme digestion of DNA 
B)  electrophoresis to separate fragments 
C)  addition of heat- stable DNA polymerase to amplify DNA 
D)  addition of a labeled probe to identify the gene of interest 
E)  transfer of DNA to nitrocellulose

Question 18

The use of an antibiotic- resistance gene on a plasmid used in genetic engineering makes

Accepted Answer

A)  the recombinant cell unable to survive. 
B)  replica plating possible. 
C)  the recombinant cell dangerous. 
D)  direct selection possible. 
E)  All of the answers are correct. 
A)  the recombinant cell unable to survive. 
B)  replica plating possible. 
C)  the recombinant cell dangerous. 
D)  direct selection possible. 
E)  All of the answers are correct.

Question 19

In recombinant DNA technology, a vector is a self- replicating segment of DNA, such as a plasmid or viral genome.

Accepted Answer

A) True 
 B)False

Question 20

Restriction enzymes are

Accepted Answer

A)  bacterial enzymes that destroy phage DNA. 
B)  animal enzymes that splice RNA. 
C)  bacterial enzymes that splice DNA. 
D)  viral enzymes that destroy host DNA. 
A)  bacterial enzymes that destroy phage DNA. 
B)  animal enzymes that splice RNA. 
C)  bacterial enzymes that splice DNA. 
D)  viral enzymes that destroy host DNA.

Exam 9: Biotechnology and Recombinant Dna

Figure 9.2 -In Figure 9.2, the enzyme in step 2 is

An advantage of cDNA over genomic DNA is that it

Biotechnology involves the

Assume you have discovered a cell that produces a lipase that works in cold water for a laundry additive. You can increase the efficiency of this enzyme by changing one amino acid. This is done by

Which of the following is NOT a desired characteristic of DNA vectors used in gene cloning procedures?

The study of genetic material taken directly from the environment is

The Ti plasmid isolated from Agrobacterium can be used to insert DNA into any type of plant.

A restriction fragment is

Which of the following is NOT an agricultural product made by DNA techniques?

An advantage of synthetic DNA over genomic or cDNA is the ability to

Which of the following places the steps in the PCR procedure in the correct order? 1) Incubate at 94°C to denature DNA strands; 2) Incubate at 72°C for DNA synthesis; 3) Incubate at 60°C for primer hybridization.

The random shotgun method is used in

The use of "suicide" genes in genetically modified organisms is designed to

Self- replicating DNA used to transmit a gene from one organism to another is a

In the Southern blot technique, which of the following is NOT required?

The use of an antibiotic- resistance gene on a plasmid used in genetic engineering makes

In recombinant DNA technology, a vector is a self- replicating segment of DNA, such as a plasmid or viral genome.

Restriction enzymes are

The Microbial World and You

Chemical Principles

Observing Microorganisms Through a Microscope

Functional Anatomy of Prokaryotic and Eukaryotic Cells

Microbial Metabolism

Microbial Growth

The Control of Microbial Growth

Microbial Genetics

Classification of Microorganisms

The Prokaryotes: Domains Bacteria and Archaea

The Eukaryotes: Fungi, Algae, Protozoa, and Helminths

Viruses, Viroids, and Prions

Principles of Disease and Epidemiology

Microbial Mechanisms of Pathogenicity

Innate Immunity: Nonspecific Defenses of the Host

Adaptive Immunity: Specific Defenses of the Host

Practical Applications of Immunology

Disorders Associated With the Immune System

Antimicrobial Drugs

Microbial Diseases of the Skin and Eyes

Microbial Diseases of the Nervous System

Microbial Diseases of the Cardiovascular and Lymphatic Systems

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Environmental Microbiology

Applied and Industrial Microbiology

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