Question 1

Which of the following are used by the Centers for Disease Control and Prevention to track outbreaks of foodborne disease?

Accepted Answer

A)  DNA fingerprints 
B)  restriction fragment length polymorphisms 
C)  reverse- transcriptase PCR (rtPCR) 
D)  DNA fingerprings and restriction fragment length polymorphisms 
E)  DNA fingerprings, restriction fragment length polymorphisms, and reverse- transcriptase PCR(rtPCR) 
A)  DNA fingerprints 
B)  restriction fragment length polymorphisms 
C)  reverse- transcriptase PCR (rtPCR) 
D)  DNA fingerprings and restriction fragment length polymorphisms 
E)  DNA fingerprings, restriction fragment length polymorphisms, and reverse- transcriptase PCR(rtPCR)

Question 2

The restriction enzyme EcoRI recognizes the sequence G$AATTC. Which of the following is TRUE of DNA after it is treated with EcoRI?

Accepted Answer

A)  All of the DNA fragments will have single- stranded regions ending in AA. 
B)  All of the DNA will have blunt ends. 
C)  All of the DNA fragments will have single- stranded regions ending in G. 
D)  All of the DNA will be circular. 
E)  Some of the DNA will have single- stranded regions ending in AA and others will end in G. 
A)  All of the DNA fragments will have single- stranded regions ending in AA. 
B)  All of the DNA will have blunt ends. 
C)  All of the DNA fragments will have single- stranded regions ending in G. 
D)  All of the DNA will be circular. 
E)  Some of the DNA will have single- stranded regions ending in AA and others will end in G.

Question 3

How many pieces will EcoRI produce from the plasmid shown in Figure 9.1?

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  4 
E)  5 
A)  1 
B)  2 
C)  3 
D)  4 
E)  5

Question 4

A population of cells carrying a desired plasmid is called a

Accepted Answer

A)  clone. 
B)  vector. 
C)  library. 
D)  PCR. 
E)  Southern blot. 
A)  clone. 
B)  vector. 
C)  library. 
D)  PCR. 
E)  Southern blot.

Question 5

The Human Genome Project, which was completed in 2003, was focused on

Accepted Answer

A)  determining all of the proteins encoded by the human genome. 
B)  determining the nucleotide sequence of the entire human genome. 
C)  cloning all of the genes of the human genome. 
D)  finding a cure for all human genetic disorders. 
E)  identifying all of the genes in the human genome. 
A)  determining all of the proteins encoded by the human genome. 
B)  determining the nucleotide sequence of the entire human genome. 
C)  cloning all of the genes of the human genome. 
D)  finding a cure for all human genetic disorders. 
E)  identifying all of the genes in the human genome.

Question 6

The following steps must be performed to make a bacterium produce human protein X. 1- Translation
2- Restriction enzyme
3- Prokaryotic transcription
4- DNA ligase
5- Transformation
6- Eukaryotic transcription
7- Reverse transcription
Which of the following places the steps in the correct order?

Accepted Answer

A)  6, 7, 2, 4, 5, 3, 1 
B)  1, 2, 3, 5, 4, 7, 6 
C)  5, 2, 3, 4, 7, 6, 1 
D)  6, 7, 2, 3, 4, 5, 1 
E)  6, 2, 1, 3, 4, 5, 7 
A)  6, 7, 2, 4, 5, 3, 1 
B)  1, 2, 3, 5, 4, 7, 6 
C)  5, 2, 3, 4, 7, 6, 1 
D)  6, 7, 2, 3, 4, 5, 1 
E)  6, 2, 1, 3, 4, 5, 7

Question 7

C) contains selectable markers.

Accepted Answer

D)  is very easy to isolate. 
E)  lacks introns.
Answer: E Explanation: 
A)  
B)  
C)  
D)  
E) 
Figure 9.2
  
-In Figure 9.2, the enzyme in step 1 is 
A)  RNA polymerase. 
B)  DNA ligase. 
C)  reverse transcriptase. 
D)  DNA polymerase. 
E)  spliceosome. 
D)  is very easy to isolate. 
E)  lacks introns.
Answer: E Explanation: 
A)  
B)  
C)  
D)  
E) 
Figure 9.2
  
-In Figure 9.2, the enzyme in step 1 is 
A)  RNA polymerase. 
B)  DNA ligase. 
C)  reverse transcriptase. 
D)  DNA polymerase. 
E)  spliceosome.

Question 8

A source of heat- stable DNA polymerase is

Accepted Answer

A)  Saccharomyces cerevisiae. 
B)  Pseudomonas. 
C)  Bacillus thuringiensis. 
D)  Thermus aquaticus. 
E)  Agrobacterium tumefaciens. 
A)  Saccharomyces cerevisiae. 
B)  Pseudomonas. 
C)  Bacillus thuringiensis. 
D)  Thermus aquaticus. 
E)  Agrobacterium tumefaciens.

Question 9

Figure 9.5
  
-Which of the following methods would be used to introduce the plasmid shown in Figure 9.5 into E. coli?

Accepted Answer

A)  microinjection 
B)  Ti plasmids and Agrobacterium 
C)  gene guns 
D)  transformation 
A)  microinjection 
B)  Ti plasmids and Agrobacterium 
C)  gene guns 
D)  transformation

Question 10

The term biotechnology refers exclusively to the use of genetically engineered organisms for the production of desired products.

Accepted Answer

A) True 
 B)False

Question 11

A colleague has used computer modeling to design an improved enzyme. To produce this enzyme, the next step is to

Accepted Answer

A)  use siRNA to produce the enzyme. 
B)  synthesize the gene for the improved enzyme. 
C)  determine the nucleotide sequence for the improved enzyme. 
D)  mutate bacteria until one makes the improved enzyme. 
E)  look for a bacterium that makes the improved enzyme. 
A)  use siRNA to produce the enzyme. 
B)  synthesize the gene for the improved enzyme. 
C)  determine the nucleotide sequence for the improved enzyme. 
D)  mutate bacteria until one makes the improved enzyme. 
E)  look for a bacterium that makes the improved enzyme.

Question 12

You have a small gene that you wish replicated by PCR. After 3 replication cycles, how many double- stranded DNA molecules do you have?

Accepted Answer

A)  2 
B)  4 
C)  8 
D)  16 
E)  thousands 
A)  2 
B)  4 
C)  8 
D)  16 
E)  thousands

Question 13

The reaction catalyzed by reverse transcriptase is

Accepted Answer

A)  tRNA -mRNA. 
B)  DNA -DNA. 
C)  DNA -mRNA. 
D)  mRNA -protein. 
E)  mRNA -cDNA. 
A)  tRNA -mRNA. 
B)  DNA -DNA. 
C)  DNA -mRNA. 
D)  mRNA -protein. 
E)  mRNA -cDNA.

Question 14

Pseudomonas syringae is found naturally in the soil. Sold as Snomax, it is used to make snow at ski resorts. The same bacterium with a gene deletion (Ice- minus) is used to prevent ice formation on plants. Should Snomax and Ice- minus be considered modified organisms and subject to precautions of releasing genetically modified organisms? Explain why or why not.

Accepted Answer

Yes, both Snomax and Ice-minus should be

Question 15

The following are steps used to make DNA fingerprints. What is the third step?

Accepted Answer

A)  Digest with a restriction enzyme. 
B)  Collect DNA. 
C)  Lyse cells. 
D)  Add stain. 
E)  Perform electrophoresis. 
A)  Digest with a restriction enzyme. 
B)  Collect DNA. 
C)  Lyse cells. 
D)  Add stain. 
E)  Perform electrophoresis.

Question 16

PCR can be used to identify an unknown bacterium because

Accepted Answer

A)  the RNA primer is specific. 
B)  DNA polymerase will replicate DNA. 
C)  DNA can be electrophoresed. 
D)  all cells have DNA. 
E)  all cells have RNA. 
A)  the RNA primer is specific. 
B)  DNA polymerase will replicate DNA. 
C)  DNA can be electrophoresed. 
D)  all cells have DNA. 
E)  all cells have RNA.

Question 17

Figure 9.3
  
-The figure at the left in Figure 9.3 shows a gene identified by Southern blotting. What will a Southern blot of the same gene look like after PCR?

Accepted Answer

A)  a 
B)  b 
C)  c 
D)  d 
E)  e 
A)  a 
B)  b 
C)  c 
D)  d 
E)  e

Question 18

Gene silencing blocks an undesirable product by

Accepted Answer

A)  making double- stranded RNA. 
B)  blocking transcription. 
C)  blocking DNA replication. 
D)  allosteric inhibition of an enzyme. 
E)  end- product repression. 
A)  making double- stranded RNA. 
B)  blocking transcription. 
C)  blocking DNA replication. 
D)  allosteric inhibition of an enzyme. 
E)  end- product repression.

Question 19

Which of the following processes is NOT involved in making cDNA?

Accepted Answer

A)  RNA processing to remove introns 
B)  transcription 
C)  translation 
D)  reverse transcription 
A)  RNA processing to remove introns 
B)  transcription 
C)  translation 
D)  reverse transcription

Question 20

One of the first commercial successes of recombinant DNA technology was the production of human insulin using genetically engineered E. coli.

Accepted Answer

A) True 
 B)False

Exam 9: Biotechnology and Recombinant Dna

Which of the following are used by the Centers for Disease Control and Prevention to track outbreaks of foodborne disease?

The restriction enzyme EcoRI recognizes the sequence G$AATTC. Which of the following is TRUE of DNA after it is treated with EcoRI?

How many pieces will EcoRI produce from the plasmid shown in Figure 9.1?

A population of cells carrying a desired plasmid is called a

The Human Genome Project, which was completed in 2003, was focused on

The following steps must be performed to make a bacterium produce human protein X. 1- Translation 2- Restriction enzyme 3- Prokaryotic transcription 4- DNA ligase 5- Transformation 6- Eukaryotic transcription 7- Reverse transcription Which of the following places the steps in the correct order?

C) contains selectable markers. D) is very easy to isolate. E) lacks introns. Answer: E Explanation: A) B) C) D) E) Figure 9.2 -In Figure 9.2, the enzyme in step 1 is

A source of heat- stable DNA polymerase is

Figure 9.5 -Which of the following methods would be used to introduce the plasmid shown in Figure 9.5 into E. coli?

The term biotechnology refers exclusively to the use of genetically engineered organisms for the production of desired products.

A colleague has used computer modeling to design an improved enzyme. To produce this enzyme, the next step is to

You have a small gene that you wish replicated by PCR. After 3 replication cycles, how many double- stranded DNA molecules do you have?

The reaction catalyzed by reverse transcriptase is

The following are steps used to make DNA fingerprints. What is the third step?

PCR can be used to identify an unknown bacterium because

Figure 9.3 -The figure at the left in Figure 9.3 shows a gene identified by Southern blotting. What will a Southern blot of the same gene look like after PCR?

Gene silencing blocks an undesirable product by

Which of the following processes is NOT involved in making cDNA?

One of the first commercial successes of recombinant DNA technology was the production of human insulin using genetically engineered E. coli.

The Microbial World and You

Chemical Principles

Observing Microorganisms Through a Microscope

Functional Anatomy of Prokaryotic and Eukaryotic Cells

Microbial Metabolism

Microbial Growth

The Control of Microbial Growth

Microbial Genetics

Classification of Microorganisms

The Prokaryotes: Domains Bacteria and Archaea

The Eukaryotes: Fungi, Algae, Protozoa, and Helminths

Viruses, Viroids, and Prions

Principles of Disease and Epidemiology

Microbial Mechanisms of Pathogenicity

Innate Immunity: Nonspecific Defenses of the Host

Adaptive Immunity: Specific Defenses of the Host

Practical Applications of Immunology

Disorders Associated With the Immune System

Antimicrobial Drugs

Microbial Diseases of the Skin and Eyes

Microbial Diseases of the Nervous System

Microbial Diseases of the Cardiovascular and Lymphatic Systems

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Environmental Microbiology

Applied and Industrial Microbiology

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