Question 1

Figure 9.5
  
-In Figure 9.5, the gene that allows the plasmid to be self- replicating is

Accepted Answer

A)  ampR. 
B)  HindIII. 
C)  ori. 
D)  lacZ. 
E)  EcoRI. 
A)  ampR. 
B)  HindIII. 
C)  ori. 
D)  lacZ. 
E)  EcoRI.

Question 2

Which of the following are used to silence specific genes and hold promise for treating cancer or viral diseases, such as hepatitis B?

Accepted Answer

A)  RNA interference (RNAi) 
B)  DNA fingerprinting 
C)  complementary DNA (cDN
A)  
D)  reverse transcriptase PCR (rtPCR) 
E)  tumor- inducing plasmids (Ti plasmids) 
A)  RNA interference (RNAi) 
B)  DNA fingerprinting 
C)  complementary DNA (cDN
A)  
D)  reverse transcriptase PCR (rtPCR) 
E)  tumor- inducing plasmids (Ti plasmids)

Question 3

Pieces of DNA stored in yeast cells are called a

Accepted Answer

A)  PCR. 
B)  Southern blot. 
C)  library. 
D)  clone. 
E)  vector. 
A)  PCR. 
B)  Southern blot. 
C)  library. 
D)  clone. 
E)  vector.

Question 4

A shuttle vector is a plasmid that is used to move pieces of DNA among organisms, such as bacterial, fungal, and plant cells.

Accepted Answer

A) True 
 B)False

Question 5

Which enzyme would cut this strand of DN?

Accepted Answer

The answer of Which enzyme would cut this strand of...

Question 6

The disadvantage of genomic libraries over cDNA libraries is that genomic libraries contain gene introns.

Accepted Answer

A) True 
 B)False

Question 7

Figure 9.5
  
-In Figure 9.5, the marker genes used for selecting recombinant DNA are

Accepted Answer

A)  lacZ and ori. 
B)  ampR and ori. 
C)  ori. 
D)  ampR and lacZ. 
E)  HindIII, BamHI, and EcoRI. 
A)  lacZ and ori. 
B)  ampR and ori. 
C)  ori. 
D)  ampR and lacZ. 
E)  HindIII, BamHI, and EcoRI.

Question 8

Figure 9.4
  
-In Figure 9.4, the bacteria transformed with the recombinant plasmid and plated on media containing ampicillin and X- gal will

Accepted Answer

A)  form blue, ampicillin- sensitive colonies. 
B)  form white, ampicillin- resistant colonies. 
C)  form white, ampicillin- sensitive colonies. 
D)  form blue, ampicillin- resistant colonies. 
E)  not grow. 
A)  form blue, ampicillin- sensitive colonies. 
B)  form white, ampicillin- resistant colonies. 
C)  form white, ampicillin- sensitive colonies. 
D)  form blue, ampicillin- resistant colonies. 
E)  not grow.

Question 9

Nearly all cells, including E. coli and yeast, naturally take up DNA from their surroundings without chemical treatment.

Accepted Answer

A) True 
 B)False

Question 10

You want to determine whether a person has a certain mutant gene. The process involves using a primer and a heat- stable DNA polymerase. This process is

Accepted Answer

A)  restriction mapping. 
B)  site- directed mutagenesis. 
C)  translation. 
D)  PCR. 
E)  transformation. 
A)  restriction mapping. 
B)  site- directed mutagenesis. 
C)  translation. 
D)  PCR. 
E)  transformation.

Question 11

Bioinformatics is the use of computer technology to compare and analyze genome sequence.

Accepted Answer

A) True 
 B)False

Question 12

If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is

Accepted Answer

A)  inserting the Ti plasmid into Agrobacterium. 
B)  inserting the Ti plasmid into a plant cell. 
C)  splicing T DNA into a plasmid. 
D)  transformation of E. coli with Ti plasmid. 
E)  transformation of an animal cell. 
A)  inserting the Ti plasmid into Agrobacterium. 
B)  inserting the Ti plasmid into a plant cell. 
C)  splicing T DNA into a plasmid. 
D)  transformation of E. coli with Ti plasmid. 
E)  transformation of an animal cell.

Question 13

Which of the following techniques is NOT used to introduce recombinant DNA into plants?

Accepted Answer

A)  gene guns 
B)  electroporation 
C)  protoplast fusion 
D)  Ti plasmids and Agrobacterium 
E)  microinjection 
A)  gene guns 
B)  electroporation 
C)  protoplast fusion 
D)  Ti plasmids and Agrobacterium 
E)  microinjection

Question 14

Figure 9.1
  
-In Figure 9.1, after digestion with the appropriate restriction enzyme, what is the smallest piece containing the ampicillin- resistance (amp) gene?

Accepted Answer

A)  0.17 kbp 
B)  0.25 kbp 
C)  1.08 kbp 
D)  1.50 kbp 
E)  3.00 kbp 
A)  0.17 kbp 
B)  0.25 kbp 
C)  1.08 kbp 
D)  1.50 kbp 
E)  3.00 kbp

Question 15

Which of the following is an advantage of using E. coli to make a human gene product?

Accepted Answer

A)  Endotoxin may be in the product. 
B)  It cannot process introns. 
C)  Its genes are well known. 
D)  It does not secrete most proteins. 
E)  Endotoxin may be in the product and it does not secrete most proteins. 
A)  Endotoxin may be in the product. 
B)  It cannot process introns. 
C)  Its genes are well known. 
D)  It does not secrete most proteins. 
E)  Endotoxin may be in the product and it does not secrete most proteins.

Question 16

The Bt toxin derived from Bacillus thuringiensis has been introduced into some crop plants to make them resistant to insect destruction.

Accepted Answer

A) True 
 B)False

Question 17

The practice of breeding plants and animals for desirable traits, such as high crop yield, is called natural selection.

Accepted Answer

A) True 
 B)False

Exam 9: Biotechnology and Recombinant Dna

Figure 9.5 -In Figure 9.5, the gene that allows the plasmid to be self- replicating is

Which of the following are used to silence specific genes and hold promise for treating cancer or viral diseases, such as hepatitis B?

Pieces of DNA stored in yeast cells are called a

A shuttle vector is a plasmid that is used to move pieces of DNA among organisms, such as bacterial, fungal, and plant cells.

Which enzyme would cut this strand of DN?

The disadvantage of genomic libraries over cDNA libraries is that genomic libraries contain gene introns.

Figure 9.5 -In Figure 9.5, the marker genes used for selecting recombinant DNA are

Figure 9.4 -In Figure 9.4, the bacteria transformed with the recombinant plasmid and plated on media containing ampicillin and X- gal will

Nearly all cells, including E. coli and yeast, naturally take up DNA from their surroundings without chemical treatment.

You want to determine whether a person has a certain mutant gene. The process involves using a primer and a heat- stable DNA polymerase. This process is

Bioinformatics is the use of computer technology to compare and analyze genome sequence.

If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is

Which of the following techniques is NOT used to introduce recombinant DNA into plants?

Figure 9.1 -In Figure 9.1, after digestion with the appropriate restriction enzyme, what is the smallest piece containing the ampicillin- resistance (amp) gene?

Which of the following is an advantage of using E. coli to make a human gene product?

The Bt toxin derived from Bacillus thuringiensis has been introduced into some crop plants to make them resistant to insect destruction.

The practice of breeding plants and animals for desirable traits, such as high crop yield, is called natural selection.

The Microbial World and You

Chemical Principles

Observing Microorganisms Through a Microscope

Functional Anatomy of Prokaryotic and Eukaryotic Cells

Microbial Metabolism

Microbial Growth

The Control of Microbial Growth

Microbial Genetics

Classification of Microorganisms

The Prokaryotes: Domains Bacteria and Archaea

The Eukaryotes: Fungi, Algae, Protozoa, and Helminths

Viruses, Viroids, and Prions

Principles of Disease and Epidemiology

Microbial Mechanisms of Pathogenicity

Innate Immunity: Nonspecific Defenses of the Host

Adaptive Immunity: Specific Defenses of the Host

Practical Applications of Immunology

Disorders Associated With the Immune System

Antimicrobial Drugs

Microbial Diseases of the Skin and Eyes

Microbial Diseases of the Nervous System

Microbial Diseases of the Cardiovascular and Lymphatic Systems

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Environmental Microbiology

Applied and Industrial Microbiology

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