Question 1

In the ssRNA phage MS2,the replicase protein binds to the start codon of the coat protein gene.Which of the following describes the reason for this binding?

Accepted Answer

A) It prevents replication and translation from occurring simultaneously. 
B) It initiates synthesis of the minus-strand copy of the genome. 
C) It enhances binding of the ribosome to the coat protein gene. 
D) It disrupts the stem-loop structure that prevents replication of the viral genome. 
E) It exposes the 3' end of the genome which allows the replicase to begin RNA synthesis. 
A) It prevents replication and translation from occurring simultaneously. 
B) It initiates synthesis of the minus-strand copy of the genome. 
C) It enhances binding of the ribosome to the coat protein gene. 
D) It disrupts the stem-loop structure that prevents replication of the viral genome. 
E) It exposes the 3' end of the genome which allows the replicase to begin RNA synthesis.

Question 2

How is the structure of an RNA phage virion different from the structure of the virion of many dsDNA phages?

Accepted Answer

A) They have much shorter tails than the DNA phages. 
B) The heads do not use icosahedral symmetry. 
C) The icosahedral head has a very large triangulation number. 
D) They are solely composed of a helical nucleocapsid. 
E) They lack the tail structure used to inject the genome into the host cell. 
A) They have much shorter tails than the DNA phages. 
B) The heads do not use icosahedral symmetry. 
C) The icosahedral head has a very large triangulation number. 
D) They are solely composed of a helical nucleocapsid. 
E) They lack the tail structure used to inject the genome into the host cell.

Question 3

Which of the following structures found on E.coli cells is required for infection with a ssRNA phage?

Accepted Answer

A) Cell wall 
B) Lipopolysaccharide 
C) F-pili 
D) Cell surface receptor 
E) All of the above are required. 
A) Cell wall 
B) Lipopolysaccharide 
C) F-pili 
D) Cell surface receptor 
E) All of the above are required.

Question 4

In the ssRNA phage MS2,the maturation protein is only produced after which of the following events occurs?

Accepted Answer

A) The synthesis of the negative strand of the viral genome. 
B) The synthesis of the positive strand of the viral genome. 
C) Packaging of the viral genome into new capsids. 
D) Translation of the coat protein. 
E) Folding of the newly synthesized genome into the stable secondary structure. 
A) The synthesis of the negative strand of the viral genome. 
B) The synthesis of the positive strand of the viral genome. 
C) Packaging of the viral genome into new capsids. 
D) Translation of the coat protein. 
E) Folding of the newly synthesized genome into the stable secondary structure.

Question 5

The viral polymerase complex from the RNA phage Q$\beta$ contains which of the following host cell proteins?

Accepted Answer

A) Host Factor 
B) S1 ribosomal protein 
C) EF-Tu translational factor 
D) EF-Ts translational factor 
E) All of the above are part of the complex. 
A) Host Factor 
B) S1 ribosomal protein 
C) EF-Tu translational factor 
D) EF-Ts translational factor 
E) All of the above are part of the complex.

Question 6

Replication and translation can occur simultaneously on a single molecule of genomic RNA from the ssRNA phage MS2.

Accepted Answer

A) True 
 B)False

Exam 5: Single-Stranded Rna Bacteriophages

In the ssRNA phage MS2,the replicase protein binds to the start codon of the coat protein gene.Which of the following describes the reason for this binding?

How is the structure of an RNA phage virion different from the structure of the virion of many dsDNA phages?

Which of the following structures found on E.coli cells is required for infection with a ssRNA phage?

In the ssRNA phage MS2,the maturation protein is only produced after which of the following events occurs?

The viral polymerase complex from the RNA phage Q β\betaβ contains which of the following host cell proteins?

Replication and translation can occur simultaneously on a single molecule of genomic RNA from the ssRNA phage MS2.

Introduction to Virology 2

Virus Structure and Assembly

Virus Classification: the World of Viruses

Virus Entry

Microviruses

Bacteriophage

Bacteriophage Lambda

Viruses of Archaea

Cucumber Mosaic Virus

Picornaviruses

Flaviviruses

Togaviruses

Coronaviruses

Paramyxoviruses and Rhabdoviruses

Filovirouses

Bunyaviruses

Influenza Viruses

Reoviruses

Parvoviruses

Polyomaviruses

Papillomaviruses

Adenoviruses

Herpesviruses

Baculoviruses

Poxviruses

Viruses of Algae and Mimivirus

Retroviruses

Human Immunodeficiency Virus

Hepadnaviruses

Viroids and Hepatitis Delta Virus

Prions

Intrinsic Cellular Defenses Against Virus Infection

Innate and Adaptive Immune Responses to Virus Infection

Antiviral Vaccines

Antiviral Chemotherapy

Eukaryotic Virus Vectors

Filters

The viral polymerase complex from the RNA phage Q $\beta$ contains which of the following host cell proteins?