Exam 9: What Genes Are

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The polymerase chain reaction (PCR)is used to

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A species that lacks DNA repair mechanisms is likely to have ________ variations between individuals than a species that possesses repair mechanisms because ________.

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Replication of DNA cannot begin until ________ bonds between ________ are broken.

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The CRISPR-Cas9 system is made up of two guide ________ molecules and two Cas9 ________ molecules.

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A detective finds a miniscule spot of blood on a murder suspect's shirt.What method would the police lab be expected to use to get enough DNA from the blood droplet to perform DNA fingerprinting?

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The difference between the alleles of a single gene is

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The polymerase chain reaction (PCR)technique could be developed only after the discovery of bacterial enzymes that were able to withstand high temperatures.Why was it necessary to use such enzymes?

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A chemical that disrupts hydrogen bonds is added to a test tube containing DNA.What would be the result of this reaction?

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A segment of DNA in a test tube replicates and produces many copies of itself.What was probably in the test tube that enhanced this process?

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The first human trial using CRISPR and approved by NIH used the system to edit

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Once attached to the proper DNA sequence,Cas9 proteins found in the CRISPR-Cas9 system ________ both strands of DNA.

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The sequence of DNA differs among individuals within a species.This is the reason for

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When a cell divides,it

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Cooling cycles during PCR amplification of DNA enable enzymes to build two new strands of ________ through ________ base-pairing.

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Along one side of a DNA molecule,the nucleotides are connected to each other by ________ bounds between ________.

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DNA fingerprinting is based on regions of DNA that are variable between individuals.Many of these differences between individuals produce no change in phenotype.This type of mutation is a(n)

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A protein binds to the outside of a DNA strand.This protein will NOT likely bind to the ________ of the DNA nucleotides.

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A rapidly dividing cell is exposed to a chemical that converts the bond that forms between adenine and thymine to a stronger covalent bond.Which of the following is MOST likely to occur?

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