Exam 7: Archael and Eukaryotic Molecular Biology

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From the standpoint of molecular biology, two similarities between the Archaea and the Eukarya are________ and _________ .

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the use of histones for DNA packaging / the detailed mechanism of mRNA translation /chromosome replication (any two)

Compare and contrast the processes of meiosis and mitosis.

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Mitosis is an asexual process used to produce two identical daughter cells from one parent cell. It maintains the number of chromosome sets in a cell where the chromosomes are segregated and condensed after DNA is replicated. Meiosis also produces cells from one parent cell but does not maintain the same number of chromosome sets and instead gives half of the chromosome set to one daughter cell and another set to the other daughter cell. The meiosis process undergoes two cell divisions where four daughter cells are produced rather than two cells produced from one cell division in mitosis. The fate of the daughter cells is different after the two processes where mitosis generates more mature cells and meiosis makes gametes for sexual reproduction.

Healthy eukaryotic cells contain all of the following EXCEPT

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D

Using humans as an example, explain the terms haploid and diploid and state when each is present.

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Chromosomal DNA at regular intervals contains condensed DNA complexes, in which DNA is wrapped around a histone tetramer in Archaea and a histone octamer in Eukarya, and are called

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DNA packaging in Archaea resembles both Bacteria and Eukarya. In what way(s) is DNA packaging analogous to Bacteria and in what way(s) is it analogous to Eukarya?

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Comparative rRNA gene sequencing has revealed a closer genetic relationship between Archaea and Bacteria than between Archaea and Eukarya.

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RNAi is triggered by dsDNA of greater than 20 bp in length.

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snRNPs are involved in the process of________.

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Several genes encoding ________and ________of Archaea possess introns that must be removed following transcription to generate the mature molecules.

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Compare and contrast RNA interference (RNAi) and restriction endonucleases.

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Positive supercoiling protects archaeal DNA against ultraviolet (UV) radiation.

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An mRNA transcript may be polycistronic in

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Compare and contrast enzymes and ribozymes of self- splicing introns.

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All DNA polymerases must add each nucleotide to a(n)________ , which is often denoted as the________ end.

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The translational machinery of Archaea is more similar to Bacteria than to Eukarya.

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Technically, telomerase is an unusual type of reverse transcriptase.

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Explain the necessity for a protein primer during replication of linear DNA.

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Which enzyme aids in replication of DNA at the guanine- rich 3' end of a strand of DNA?

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The 5.8S rRNA is a ribosomal protein found in________ but not in ________.

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