Question 1

The gene products of the lacZ, lacA and lacY genes are specific for the hydrolysis of a different
β-galactoside sugar.

Accepted Answer

A) True 
 B)False

Question 2

Which of the following is an incorrect association between transcription factors and their characteristics?

Accepted Answer

A)  TFIIB : interacts with RNA polymerase II. 
B)  SPI: binds to GC-rich sequence. 
C)  TFIIH: couples transcription to DNA repair. 
D)  TFIIE : recognizes the core sequence CCAAT. 
E)  All of the above 
A)  TFIIB : interacts with RNA polymerase II. 
B)  SPI: binds to GC-rich sequence. 
C)  TFIIH: couples transcription to DNA repair. 
D)  TFIIE : recognizes the core sequence CCAAT. 
E)  All of the above

Question 3

The type of RNA that is least stable in cells is .

Accepted Answer

A)  tRNA 
B)  rRNA 
C)  mRNA 
D)  small RNA 
A)  tRNA 
B)  rRNA 
C)  mRNA 
D)  small RNA

Question 4

Which of the following is true, concerning rho?

Accepted Answer

A)  It is a heptameric protein with potent ATPase activity. 
B)  It has an affinity for single-stranded DNA . 
C)  It may function as an RNA-DNA helicase. 
D)  None of the above 
A)  It is a heptameric protein with potent ATPase activity. 
B)  It has an affinity for single-stranded DNA . 
C)  It may function as an RNA-DNA helicase. 
D)  None of the above

Question 5

Most of the RNA polymerase activity in eukaryotic cells occurs in the

Accepted Answer

A)  cytoplasm. 
B)  mitochondria. 
C)  nucleolus. 
D)  nucleoplasm. 
A)  cytoplasm. 
B)  mitochondria. 
C)  nucleolus. 
D)  nucleoplasm.

Question 6

Which has its primary transcript produced in the nucleolus?

Accepted Answer

A)  tRNA 
B)  mRNA 
C)  rRNA 
D)  sRNA 
A)  tRNA 
B)  mRNA 
C)  rRNA 
D)  sRNA

Question 7

When a repressor protein is bound, unbound) to a promoter region transcription is bound, unbound) to a promoter transcription is

Accepted Answer

A)  bound; unbound 
B)  unbound; bound 
C)  bound; bound 
D)  unbound; unbound 
A)  bound; unbound 
B)  unbound; bound 
C)  bound; bound 
D)  unbound; unbound

Question 8

Activators and repressors are often allosteric proteins.

Accepted Answer

A) True 
 B)False

Question 9

RNA polymerase uses the strand of DNA in transcription.

Accepted Answer

A)  sense 
B)  antisense 
C)  coding 
D)  un-coded 
A)  sense 
B)  antisense 
C)  coding 
D)  un-coded

Question 10

Rho-dependent termination requires exposure of single-stranded RNA.

Accepted Answer

A) True 
 B)False

Question 11

Which does not apply to the post-transcriptional processing of ribosomal RNA?

Accepted Answer

A)  The subunits of rRNA are produced in equal amounts since they originate from the same transcript. 
B)  tRNA precursors are sometimes cleaved from the primary transcript of rRNA precursor. 
C)  A polyA tail is added to the 5ʹ end of the 30S subunit. 
D)  After initial cleavage of the primary transcript, specific endonucleases trim nucleotides from the ends of rRNA molecules. 
A)  The subunits of rRNA are produced in equal amounts since they originate from the same transcript. 
B)  tRNA precursors are sometimes cleaved from the primary transcript of rRNA precursor. 
C)  A polyA tail is added to the 5ʹ end of the 30S subunit. 
D)  After initial cleavage of the primary transcript, specific endonucleases trim nucleotides from the ends of rRNA molecules.

Question 12

A transcription complex does not form when one or more proteins bind to the promoter sequence and to RNA polymerase in eukaryotes.

Accepted Answer

A) True 
 B)False

Question 13

The large RNA-protein complex that processes introns and exons is called .

Accepted Answer

A)  the replisome 
B)  the catalytic RNA complex 
C)  the spliceosome 
D)  RNA ligase 
A)  the replisome 
B)  the catalytic RNA complex 
C)  the spliceosome 
D)  RNA ligase

Question 14

Mutations in sequences that are removed from the primary RNA transcript never affect RNA processing.

Accepted Answer

A) True 
 B)False

Question 15

The splicing of exons does not occur in prokaryotes.

Accepted Answer

A) True 
 B)False

Question 16

When a sequence of double-strand DNA is displayed

Accepted Answer

A)  the coding strand is written in the 3ʹ → 5ʹ direction. 
B)  the template strand is written in the 5ʹ → 3ʹ direction. 
C)  the coding strand is identical to the RNA product. 
D)  None of the above 
A)  the coding strand is written in the 3ʹ → 5ʹ direction. 
B)  the template strand is written in the 5ʹ → 3ʹ direction. 
C)  the coding strand is identical to the RNA product. 
D)  None of the above

Question 17

What does it mean to say that the most common type of promoter in E. coli is bipartite?

Accepted Answer

A)  The promoter region is divided into two parts which flank the sequence for the gene. 
B)  There are two separate regions of sequence similarity in the promoter. 
C)  The sequence of the promoter has dyad symmetry. 
D)  The promoter must bind two RNA polymerase holoenzymes to transcribe the gene. 
A)  The promoter region is divided into two parts which flank the sequence for the gene. 
B)  There are two separate regions of sequence similarity in the promoter. 
C)  The sequence of the promoter has dyad symmetry. 
D)  The promoter must bind two RNA polymerase holoenzymes to transcribe the gene.

Question 18

Sequences that are removed from the primary RNA transcript in eukaryotes are called .

Accepted Answer

A)  introns 
B)  operons 
C)  exons 
D)  mRNA 
A)  introns 
B)  operons 
C)  exons 
D)  mRNA

Question 19

Which of the following is an incorrect association between RNA polymerase and its products?

Accepted Answer

A)  RNA polymerase I : 35 - 47S pre-rRNA 
B)  RNA polymerase I: 5S rRNA 
C)  RNA polymerase II : mRNA precursors 
D)  RNA polymerase III : tRNA 
E)  None of the above 
A)  RNA polymerase I : 35 - 47S pre-rRNA 
B)  RNA polymerase I: 5S rRNA 
C)  RNA polymerase II : mRNA precursors 
D)  RNA polymerase III : tRNA 
E)  None of the above

Question 20

RNA polymerase catalyzes DNA-directed RNA synthesis.

Accepted Answer

A) True 
 B)False

Exam 21: Transcription and Rna Processing

The gene products of the lacZ, lacA and lacY genes are specific for the hydrolysis of a different β-galactoside sugar.

Which of the following is an incorrect association between transcription factors and their characteristics?

The type of RNA that is least stable in cells is .

Which of the following is true, concerning rho?

Most of the RNA polymerase activity in eukaryotic cells occurs in the

Which has its primary transcript produced in the nucleolus?

When a repressor protein is bound, unbound) to a promoter region transcription is bound, unbound) to a promoter transcription is

Activators and repressors are often allosteric proteins.

RNA polymerase uses the strand of DNA in transcription.

Rho-dependent termination requires exposure of single-stranded RNA.

Which does not apply to the post-transcriptional processing of ribosomal RNA?

A transcription complex does not form when one or more proteins bind to the promoter sequence and to RNA polymerase in eukaryotes.

The large RNA-protein complex that processes introns and exons is called .

Mutations in sequences that are removed from the primary RNA transcript never affect RNA processing.

The splicing of exons does not occur in prokaryotes.

When a sequence of double-strand DNA is displayed

What does it mean to say that the most common type of promoter in E. coli is bipartite?

Sequences that are removed from the primary RNA transcript in eukaryotes are called .

Which of the following is an incorrect association between RNA polymerase and its products?

RNA polymerase catalyzes DNA-directed RNA synthesis.

Introduction to Biochemistry

Water

Amino Acids and the Primary Structures of Proteins

Proteins: Three-Dimensional Structure and Function

Properties of Enzymes

Mechanisms of Enzymes

Coenzymes and Vitamins

Carbohydrates

Lipids and Membranes

Introduction to Metabolism

Glycolysis

Gluconeogenesis, the Pentose Phosphate Pathway, and Glycogen Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Nucleic Acids

Dna Replication, Repair, and Recombination

Protein Synthesis

Recombinant Dna Technology

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