Question 1

Rho-dependent termination occurs at pause sites within the coding region.

Accepted Answer

A) True 
 B)False

Question 2

The CRP-cAMP complex is an activator of the lac operon.

Accepted Answer

A) True 
 B)False

Question 3

For an RNA hairpin loop to form, the transcribed region of the DNA sequence must .

Accepted Answer

A)  be a palindrome 
B)  have dyad symmetry 
C)  contain an intron 
D)  have a tandem repeat 
A)  be a palindrome 
B)  have dyad symmetry 
C)  contain an intron 
D)  have a tandem repeat

Question 4

A gene contains two 100 bp introns and three 50 bp exons. About how long is the mature RNA transcribed from this gene?

Accepted Answer

A)  75 bp 
B)  150 bp 
C)  200 bp 
D)  350 bp 
E)  Cannot tell from the information given 
A)  75 bp 
B)  150 bp 
C)  200 bp 
D)  350 bp 
E)  Cannot tell from the information given

Question 5

Which of the following is/are true concerning TAFs and TBP?

Accepted Answer

A)  They are subunits of TFIIB and TFIID, respectively. 
B)  TBP is required to initiate transcription of class I and class III genes . 
C)  They are not associated with RNA polymerase I and RNA polymerase III. 
D)  All of the above 
A)  They are subunits of TFIIB and TFIID, respectively. 
B)  TBP is required to initiate transcription of class I and class III genes . 
C)  They are not associated with RNA polymerase I and RNA polymerase III. 
D)  All of the above

Question 6

Which statement is false about the utilization of lactose as a carbon source by E. coli?

Accepted Answer

A)  In the presence of both glucose and lactose, the enzymes necessary for lactose catabolism are produced only in limited amounts. 
B)  Lactose is the inducer for the enzymes responsible for its own catabolism. 
C)  In the absence of lactose, the repressor binds to a single site upstream of the lac genes. 
D)  The catabolic enzymes are encoded in a three-gene operon controlled by a single promoter. 
A)  In the presence of both glucose and lactose, the enzymes necessary for lactose catabolism are produced only in limited amounts. 
B)  Lactose is the inducer for the enzymes responsible for its own catabolism. 
C)  In the absence of lactose, the repressor binds to a single site upstream of the lac genes. 
D)  The catabolic enzymes are encoded in a three-gene operon controlled by a single promoter.

Question 7

Post-transcriptional modification of mRNA in eukaryotes occurs after they are transported to the cytosol, but before they bind to the ribosome.

Accepted Answer

A) True 
 B)False

Question 8

The σ70 subunit in E. coli RNA polymerase acts as a/an .

Accepted Answer

A)  repressor 
B)  sliding clamp 
C)  helicase 
D)  initiation factor 
A)  repressor 
B)  sliding clamp 
C)  helicase 
D)  initiation factor

Question 9

TFIIB can bind to DNA in the absence of TFIID.

Accepted Answer

A) True 
 B)False

Question 10

A consensus sequence

Accepted Answer

A)  is a hypothetical sequence. 
B)  indicates the nucleotides most commonly found at each position. 
C)  describes the most efficient promoter sequence for the RNA polymerase holoenzyme. 
D)  All of the above 
A)  is a hypothetical sequence. 
B)  indicates the nucleotides most commonly found at each position. 
C)  describes the most efficient promoter sequence for the RNA polymerase holoenzyme. 
D)  All of the above

Question 11

Francois Jacob and Jacques Monod are responsible for

Accepted Answer

A)  discovering that the sequence of an mRNA molecule is complementary to a segment of one of the DNA strands. 
B)  predicting the ʺcentral dogmaʺ of molecular biology. 
C)  proposing that a single unit of heredity directed the production of a single enzyme. 
D)  All of the above 
A)  discovering that the sequence of an mRNA molecule is complementary to a segment of one of the DNA strands. 
B)  predicting the ʺcentral dogmaʺ of molecular biology. 
C)  proposing that a single unit of heredity directed the production of a single enzyme. 
D)  All of the above

Question 12

Which is not a feature of eukaryotic mRNA processing?

Accepted Answer

A)  addition of a 5ʹ-5ʹ triphosphate linkage to GTP 
B)  methylation of the terminal CCA triad 
C)  addition of a polyA tail to the 3ʹ end 
D)  removal of intron sequences 
A)  addition of a 5ʹ-5ʹ triphosphate linkage to GTP 
B)  methylation of the terminal CCA triad 
C)  addition of a polyA tail to the 3ʹ end 
D)  removal of intron sequences

Question 13

Which statement is not true about the binding of TBP TATA binding protein)?

Accepted Answer

A)  The TBP subunit is only required for the initiation of transcription of class II genes. 
B)  The binding of TBP to DNA distorts the DNA out of the standard B-DNA form. 
C)  Contacts between TBP and DNA are mainly in the minor groove. 
D)  TBP forms a saddle-shaped molecular clamp that surrounds DNA at the TATA box. 
A)  The TBP subunit is only required for the initiation of transcription of class II genes. 
B)  The binding of TBP to DNA distorts the DNA out of the standard B-DNA form. 
C)  Contacts between TBP and DNA are mainly in the minor groove. 
D)  TBP forms a saddle-shaped molecular clamp that surrounds DNA at the TATA box.

Question 14

Operons are a common gene arrangement found in all living organisms.

Accepted Answer

A) True 
 B)False

Question 15

The sigma-70 subunit

Accepted Answer

A)  increases the affinity of the core polymerase for nonpromoter sequences. 
B)  decreases the affinity of the core polymerase for specific promoter sequences. 
C)  is responsible for transcribing many genes. 
D)  is associated with a holoenzyme, enabling the formation of more stable complexes. 
E)  All of the above. 
A)  increases the affinity of the core polymerase for nonpromoter sequences. 
B)  decreases the affinity of the core polymerase for specific promoter sequences. 
C)  is responsible for transcribing many genes. 
D)  is associated with a holoenzyme, enabling the formation of more stable complexes. 
E)  All of the above.

Question 16

The same RNA polymerase that transcribes the ribosomal RNA transcribes all mitochondrial genes.

Accepted Answer

A) True 
 B)False

Question 17

Which is not part of the lac operon?

Accepted Answer

A)  lacA 
B)  lacI 
C)  lacY 
D)  lacZ 
E)  None of the above; they are all part of the lac operon 
A)  lacA 
B)  lacI 
C)  lacY 
D)  lacZ 
E)  None of the above; they are all part of the lac operon

Question 18

Transcription termination

Accepted Answer

A)  occurs at certain DNA sequences where the elongation complex is unstable. 
B)  sometimes requires a specific protein, rho, which facilitates disassembly. 
C)  often occurs near pause sites. 
D)  All of the above 
A)  occurs at certain DNA sequences where the elongation complex is unstable. 
B)  sometimes requires a specific protein, rho, which facilitates disassembly. 
C)  often occurs near pause sites. 
D)  All of the above

Question 19

Which is not generally associated with RNA processing?

Accepted Answer

A)  addition of nucleotides 
B)  removal of nucleotides 
C)  covalent modification of bases 
D)  phosphorylation at the 3ʹ end 
A)  addition of nucleotides 
B)  removal of nucleotides 
C)  covalent modification of bases 
D)  phosphorylation at the 3ʹ end

Question 20

Which of the following is true about transcription?

Accepted Answer

A)  Promoters are regions of DNA which serve as transcription initiation sites. 
B)  Initiation is the rate-limiting step in transcription. 
C)  Each gene, in eukaryotic cells, usually has its own promoter. 
D)  All of the above 
A)  Promoters are regions of DNA which serve as transcription initiation sites. 
B)  Initiation is the rate-limiting step in transcription. 
C)  Each gene, in eukaryotic cells, usually has its own promoter. 
D)  All of the above

Exam 21: Transcription and Rna Processing

Rho-dependent termination occurs at pause sites within the coding region.

The CRP-cAMP complex is an activator of the lac operon.

For an RNA hairpin loop to form, the transcribed region of the DNA sequence must .

A gene contains two 100 bp introns and three 50 bp exons. About how long is the mature RNA transcribed from this gene?

Which of the following is/are true concerning TAFs and TBP?

Which statement is false about the utilization of lactose as a carbon source by E. coli?

Post-transcriptional modification of mRNA in eukaryotes occurs after they are transported to the cytosol, but before they bind to the ribosome.

The σ70 subunit in E. coli RNA polymerase acts as a/an .

TFIIB can bind to DNA in the absence of TFIID.

A consensus sequence

Francois Jacob and Jacques Monod are responsible for

Which is not a feature of eukaryotic mRNA processing?

Which statement is not true about the binding of TBP TATA binding protein)?

Operons are a common gene arrangement found in all living organisms.

The sigma-70 subunit

The same RNA polymerase that transcribes the ribosomal RNA transcribes all mitochondrial genes.

Which is not part of the lac operon?

Transcription termination

Which is not generally associated with RNA processing?

Which of the following is true about transcription?

Introduction to Biochemistry

Water

Amino Acids and the Primary Structures of Proteins

Proteins: Three-Dimensional Structure and Function

Properties of Enzymes

Mechanisms of Enzymes

Coenzymes and Vitamins

Carbohydrates

Lipids and Membranes

Introduction to Metabolism

Glycolysis

Gluconeogenesis, the Pentose Phosphate Pathway, and Glycogen Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Nucleic Acids

Dna Replication, Repair, and Recombination

Protein Synthesis

Recombinant Dna Technology

Filters

The σ⁷⁰subunit in E. coli RNA polymerase acts as a/an .