Question 1

In a cDNA library the probability of finding the desired clone depends on .

Accepted Answer

A)  the abundance of the original mRNA 
B)  the genome size 
C)  the average length of mRNA 
D)  All of the above 
A)  the abundance of the original mRNA 
B)  the genome size 
C)  the average length of mRNA 
D)  All of the above A

Question 2

Yeast artificial chromosomes YACs are introduced into yeast cells as circular molecules.

Accepted Answer

A) True 
 B)False  False

Question 3

Results of research on the Human Genome Project should enable which of the following?

Accepted Answer

A)  precise diagnosis of disease 
B)  individual risk assessment 
C)  prediction of the course of a disease 
D)  All of the above 
A)  precise diagnosis of disease 
B)  individual risk assessment 
C)  prediction of the course of a disease 
D)  All of the above D

Question 4

Restriction fragment length polymorphisms refer to .

Accepted Answer

A)  variations in the size of mature mRNA transcripts from one individual to the next 
B)  variations in the lengths of genomic DNA fragments from a single individual from samples that are treated with different endonucleases 
C)  variations in the lengths of genomic DNA fragments among DNA from different tissues in a single individual that are treated with the same endonuclease 
D)  variations in the lengths of genomic DNA fragments among individuals that are treated with the same endonuclease 
A)  variations in the size of mature mRNA transcripts from one individual to the next 
B)  variations in the lengths of genomic DNA fragments from a single individual from samples that are treated with different endonucleases 
C)  variations in the lengths of genomic DNA fragments among DNA from different tissues in a single individual that are treated with the same endonuclease 
D)  variations in the lengths of genomic DNA fragments among individuals that are treated with the same endonuclease

Question 5

A molecule that specifically recognizes the desired piece of DNA in a large library is known as a

Accepted Answer

A)  cosmid. 
B)  plasmid. 
C)  probe. 
D)  vector. 
A)  cosmid. 
B)  plasmid. 
C)  probe. 
D)  vector.

Question 6

Which of the following is true, concerning the plasmid vector, pBR322?

Accepted Answer

A)  It contains an origin of replication ori). 
B)  It contains genes which confer resistance to the antibiotics ampicillin and tetracycline. 
C)  It contains a number of restriction sites that are useful for constructing RNA. 
D)  All of the above 
E)  Only A and B 
A)  It contains an origin of replication ori). 
B)  It contains genes which confer resistance to the antibiotics ampicillin and tetracycline. 
C)  It contains a number of restriction sites that are useful for constructing RNA. 
D)  All of the above 
E)  Only A and B

Question 7

The construction of a cDNA library is completed by

Accepted Answer

A)  purifying mRNA molecules from eukaryotes. 
B)  ligating the cDNA molecules to vectors and introducing the recombinants into host cells. 
C)  incubating the mRNA population with oligodeoxythymidylate. 
D)  adding synthetic oligonucleotides to sticky-ended cDNA molecules. 
A)  purifying mRNA molecules from eukaryotes. 
B)  ligating the cDNA molecules to vectors and introducing the recombinants into host cells. 
C)  incubating the mRNA population with oligodeoxythymidylate. 
D)  adding synthetic oligonucleotides to sticky-ended cDNA molecules.

Question 8

Which component is not necessary for a PCR experiment?

Accepted Answer

A)  free nucleotides 
B)  reverse transcriptase 
C)  RNA primers 
D)  DNA polymerase 
E)  template DNA 
A)  free nucleotides 
B)  reverse transcriptase 
C)  RNA primers 
D)  DNA polymerase 
E)  template DNA

Question 9

Restriction enzymes randomly cleave DNA.

Accepted Answer

A) True 
 B)False

Question 10

In a blue/white screen

Accepted Answer

A)  the blue colonies represent cells transformed with cloning vectors which contain inserts. 
B)  the white colonies represent cells transformed with recombinants. 
C)  the presence of blue dye identifies cells containing a disrupted a-galactosidase gene. 
D)  All of the above 
A)  the blue colonies represent cells transformed with cloning vectors which contain inserts. 
B)  the white colonies represent cells transformed with recombinants. 
C)  the presence of blue dye identifies cells containing a disrupted a-galactosidase gene. 
D)  All of the above

Question 11

A/an is often used to enable bacteria to produce large amounts of eukaryotic protein.

Accepted Answer

A)  cosmid 
B)  transgenic phage 
C)  expression vector 
D)  cDNA clone 
A)  cosmid 
B)  transgenic phage 
C)  expression vector 
D)  cDNA clone

Question 12

Chromosome walking involves the isolation and sequencing of consecutive segments of DNA isolated after treatment with a restriction endonuclease.

Accepted Answer

A) True 
 B)False

Question 13

It is possible for eukaryotic genes with introns to be isolated from genomic libraries and expressed directly in yeast cells.

Accepted Answer

A) True 
 B)False

Question 14

is a technique used to link noncontiguous recombinants from the same gene or to identify genes whose sequence and gene product are unknown other than by the effects of mutation.

Accepted Answer

A)  Transformation 
B)  Polymerase chain reaction 
C)  Antibody screening 
D)  Chromosome walking 
A)  Transformation 
B)  Polymerase chain reaction 
C)  Antibody screening 
D)  Chromosome walking

Question 15

It is possible to clone a gene for a protein that is not expressed in the tissue from which the cDNA library was derived.

Accepted Answer

A) True 
 B)False

Question 16

Which of the following list gives the amount of foreign DNA that can be inserted into vectors is not true?

Accepted Answer

A)  YAC - 400 to 500 kb 
B)  Plasmids - 20 kb 
C)  Bacteriophage l - up to 22 kb 
D)  Cosmids - up to 5 kb 
A)  YAC - 400 to 500 kb 
B)  Plasmids - 20 kb 
C)  Bacteriophage l - up to 22 kb 
D)  Cosmids - up to 5 kb

Question 17

Which of the following may function as a labeled probe?

Accepted Answer

A)  oligonucleotides 
B)  antibodies 
C)  radioactive isotopes 
D)  fluorescent dyes 
E)  All of the above 
A)  oligonucleotides 
B)  antibodies 
C)  radioactive isotopes 
D)  fluorescent dyes 
E)  All of the above

Question 18

What is the purpose of the heating cycle during PCR?

Accepted Answer

A)  activates Taq polymerase 
B)  denatures the DNA 
C)  facilitates the attachment of RNA primers 
D)  increases the rate of polymerization by increasing the rate of diffusion of free nucleotides 
A)  activates Taq polymerase 
B)  denatures the DNA 
C)  facilitates the attachment of RNA primers 
D)  increases the rate of polymerization by increasing the rate of diffusion of free nucleotides

Question 19

Inserting DNA into a cloning site within the tetR gene may cause the following

Accepted Answer

A)  inactivation of the tetR gene. 
B)  produce transformants which are sensitive to ampicillin. 
C)  produce transformants which are resistant to tetracycline. 
D)  All of the above 
A)  inactivation of the tetR gene. 
B)  produce transformants which are sensitive to ampicillin. 
C)  produce transformants which are resistant to tetracycline. 
D)  All of the above

Question 20

cDNA libraries from liver cells are different than cDNA libraries from kidney cells because

Accepted Answer

A)  the DNA is not the same in the two types of cells. 
B)  reverse transcriptase acts differently in the two types of cells. 
C)  mRNA is different in the two types of cells. 
D)  polyA is present at 3ʹ ends of mRNA in one cell type only. 
A)  the DNA is not the same in the two types of cells. 
B)  reverse transcriptase acts differently in the two types of cells. 
C)  mRNA is different in the two types of cells. 
D)  polyA is present at 3ʹ ends of mRNA in one cell type only.

Exam 23: Recombinant Dna Technology

In a cDNA library the probability of finding the desired clone depends on .

Yeast artificial chromosomes YACs are introduced into yeast cells as circular molecules.

Results of research on the Human Genome Project should enable which of the following?

Restriction fragment length polymorphisms refer to .

A molecule that specifically recognizes the desired piece of DNA in a large library is known as a

Which of the following is true, concerning the plasmid vector, pBR322?

The construction of a cDNA library is completed by

Which component is not necessary for a PCR experiment?

Restriction enzymes randomly cleave DNA.

In a blue/white screen

A/an is often used to enable bacteria to produce large amounts of eukaryotic protein.

Chromosome walking involves the isolation and sequencing of consecutive segments of DNA isolated after treatment with a restriction endonuclease.

It is possible for eukaryotic genes with introns to be isolated from genomic libraries and expressed directly in yeast cells.

is a technique used to link noncontiguous recombinants from the same gene or to identify genes whose sequence and gene product are unknown other than by the effects of mutation.

It is possible to clone a gene for a protein that is not expressed in the tissue from which the cDNA library was derived.

Which of the following list gives the amount of foreign DNA that can be inserted into vectors is not true?

Which of the following may function as a labeled probe?

What is the purpose of the heating cycle during PCR?

Inserting DNA into a cloning site within the tetR gene may cause the following

cDNA libraries from liver cells are different than cDNA libraries from kidney cells because

Introduction to Biochemistry

Water

Amino Acids and the Primary Structures of Proteins

Proteins: Three-Dimensional Structure and Function

Properties of Enzymes

Mechanisms of Enzymes

Coenzymes and Vitamins

Carbohydrates

Lipids and Membranes

Introduction to Metabolism

Glycolysis

Gluconeogenesis, the Pentose Phosphate Pathway, and Glycogen Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Nucleic Acids

Dna Replication, Repair, and Recombination

Transcription and Rna Processing

Protein Synthesis

Filters

Inserting DNA into a cloning site within the tet^Rgene may cause the following