Exam 23: Recombinant Dna Technology

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Which formula may be implemented to determine the probability P) that a library of a given size contains a particular clone? N is the number of recombinant clones in the library; n is the frequency of occurrence of a desired clone)

(Multiple Choice)

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Question 61

Cloned cDNA is used less frequently than mRNA as a probe for protein encoding genes in genomic DNA libraries.

(True/False)

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Question 62

In a genomic DNA library the frequency of occurrence of a desired clone, n, also represents

(Multiple Choice)

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Question 63

Mechanical shearing is often the preferred method of fragmenting genomic DNA when constructing a genomic DNA library. Why would this method be preferred over complete digestion with restriction enzymes?

(Multiple Choice)

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Question 64

Which of the following is an advantage of phage vectors over plasmid vectors?

(Multiple Choice)

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Question 65

Which is true about the frequency of occurrence of a desired clone from a genomic DNA library?

(Multiple Choice)

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Question 66

A mouse embryo is transformed with recombinant DNA from a rat. The resulting mouse is a organism.

(Multiple Choice)

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Question 67

are small, circular double-stranded DNA molecules used as vectors for DNA fragments up to 20kb in size.

(Multiple Choice)

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Question 68

Why is Taq polymerase used in PCR rather than DNA polymerases from other sources?

(Multiple Choice)

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Question 69

Exons and introns are equally represented in a cDNA library.

(True/False)

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Question 70

For genes that are highly conserved, probes developed from one species can be used to screen clones from many other species.

(True/False)

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Question 71

Which of the following can function as a cloning vector?

(Multiple Choice)

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Question 72

The Ti plasmid is derived from .

(Multiple Choice)

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Question 73

Showing 61 - 73 of 73

Which formula may be implemented to determine the probability P) that a library of a given size contains a particular clone? N is the number of recombinant clones in the library; n is the frequency of occurrence of a desired clone)

Cloned cDNA is used less frequently than mRNA as a probe for protein encoding genes in genomic DNA libraries.

In a genomic DNA library the frequency of occurrence of a desired clone, n, also represents

Mechanical shearing is often the preferred method of fragmenting genomic DNA when constructing a genomic DNA library. Why would this method be preferred over complete digestion with restriction enzymes?

Which of the following is an advantage of phage vectors over plasmid vectors?

Which is true about the frequency of occurrence of a desired clone from a genomic DNA library?

A mouse embryo is transformed with recombinant DNA from a rat. The resulting mouse is a organism.

are small, circular double-stranded DNA molecules used as vectors for DNA fragments up to 20kb in size.

Why is Taq polymerase used in PCR rather than DNA polymerases from other sources?

Exons and introns are equally represented in a cDNA library.

For genes that are highly conserved, probes developed from one species can be used to screen clones from many other species.

Which of the following can function as a cloning vector?

The Ti plasmid is derived from .

Introduction to Biochemistry

Water

Amino Acids and the Primary Structures of Proteins

Proteins: Three-Dimensional Structure and Function

Properties of Enzymes

Mechanisms of Enzymes

Coenzymes and Vitamins

Carbohydrates

Lipids and Membranes

Introduction to Metabolism

Glycolysis

Gluconeogenesis, the Pentose Phosphate Pathway, and Glycogen Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Nucleic Acids

Dna Replication, Repair, and Recombination

Transcription and Rna Processing

Protein Synthesis

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