Question 1

The oligodeoxythymidylate oligo dT fragment functions as a primer for synthesis of a complementary DNA strand by reverse transcriptase.

Accepted Answer

A) True 
 B)False

Question 2

Shine-Dalgarno sequences are components of plasmid vectors which help to maximize the expression of inserted genes.

Accepted Answer

A) True 
 B)False

Question 3

An individual is treated for cystic fibrosis by a recombinant virus administered through an inhaler so the virus can ʺinfectʺ the cells lining the lungs. In the lungs, the recombinant DNA is incorporated into the patientʹs genomic DNA and produces a protein product that helps relieve the symptoms of the disease. Children born to this patient after the treatment can be expected to also carry the recombinant genes and benefit from them if they too suffer from cystic fibrosis.

Accepted Answer

A) True 
 B)False

Question 4

Changing factors such as the salt concentration and temperature can be used to vary the stringency of hybridization and allow for more or less mismatches in base pairing.

Accepted Answer

A) True 
 B)False

Question 5

To discover whether a species has a gene that is similar but not identical to the cDNA of another species, one can

Accepted Answer

A)  prepare proteins from one species only. 
B)  isolate the two different genes first. 
C)  hybridize using histone. 
D)  alter the temperature and salt concentrations used in the hybridization. 
A)  prepare proteins from one species only. 
B)  isolate the two different genes first. 
C)  hybridize using histone. 
D)  alter the temperature and salt concentrations used in the hybridization.

Question 6

Which of the following is true concerning genomic libraries?

Accepted Answer

A)  Genomic libraries often use cloning vectors derived from plasmid vectors. 
B)  Total restriction digestion frequently is used to ensure that the library contains every gene. 
C)  Genomic libraries include both expressed and non-expressed DNA from an organism. 
D)  All of the above 
A)  Genomic libraries often use cloning vectors derived from plasmid vectors. 
B)  Total restriction digestion frequently is used to ensure that the library contains every gene. 
C)  Genomic libraries include both expressed and non-expressed DNA from an organism. 
D)  All of the above

Question 7

In general, when constructing a YAC library of genomic DNA the smaller the insert used the fewer clones are needed.

Accepted Answer

A) True 
 B)False

Question 8

Bacterial artificial chromosomes BACs) require in order to successfully enter into bacterial cells.

Accepted Answer

A)  transfection 
B)  electroporation 
C)  the use of complementary nucleotides 
D)  linkers 
A)  transfection 
B)  electroporation 
C)  the use of complementary nucleotides 
D)  linkers

Question 9

Which is not an important feature of site-directed mutagenesis of cloned DNA?

Accepted Answer

A)  It can be used to introduce point mutations. 
B)  It is faster than the complete in vitro synthesis of mutant genes. 
C)  It can be used to exponentially amplify the amount of DNA in a sample. 
D)  It allows for easy screening of transformants by using labeled oligonucleotides. 
A)  It can be used to introduce point mutations. 
B)  It is faster than the complete in vitro synthesis of mutant genes. 
C)  It can be used to exponentially amplify the amount of DNA in a sample. 
D)  It allows for easy screening of transformants by using labeled oligonucleotides.

Question 10

Plasmids are usually introduced into bacterial cells by .

Accepted Answer

A)  transfusion 
B)  transfection 
C)  transduction 
D)  transformation 
A)  transfusion 
B)  transfection 
C)  transduction 
D)  transformation

Question 11

replicate in either prokaryotic or eukaryotic cells.

Accepted Answer

A)  Phage vectors 
B)  Shuttle vectors 
C)  Cosmids 
D)  Transposons 
A)  Phage vectors 
B)  Shuttle vectors 
C)  Cosmids 
D)  Transposons

Question 12

RFLP analysis is a very useful technique for genetic analysis and forensic studies, but it is not precise enough to distinguish genetic differences between highly related individuals such as siblings or parents and children.

Accepted Answer

A) True 
 B)False

Question 13

Who won the Nobel Prize in 1993 for his work on site-directed mutagenesis?

Accepted Answer

A)  Kary Mullis 
B)  Michael Smith 
C)  Herbert Boyer 
D)  James Watson 
A)  Kary Mullis 
B)  Michael Smith 
C)  Herbert Boyer 
D)  James Watson

Question 14

RFLPs are very useful in determining paternity.

Accepted Answer

A) True 
 B)False

Question 15

After ten cycles of PCR, by approximately what factor will the number of DNA molecules in a sample be increased? Assume maximum efficiency.

Accepted Answer

A)  10 
B)  20 
C)  1000 
D)  10,000,000,000 
A)  10 
B)  20 
C)  1000 
D)  10,000,000,000

Question 16

It can be difficult for bacteria to produce functional eukaryotic proteins even when all the components necessary for gene expression are present because .

Accepted Answer

A)  eukaryotes do not use the Shine-Dalgarno sequence 
B)  many eukaryotic proteins must be post-translationally modified 
C)  of the size difference between eukaryotic and prokaryotic ribosomes 
D)  of differences in the prokaryotic and eukaryotic genetic codes 
A)  eukaryotes do not use the Shine-Dalgarno sequence 
B)  many eukaryotic proteins must be post-translationally modified 
C)  of the size difference between eukaryotic and prokaryotic ribosomes 
D)  of differences in the prokaryotic and eukaryotic genetic codes

Question 17

Which of the following statements is true concerning sticky ends?

Accepted Answer

A)  They are fragments with single strand extensions at only the 5ʹ end of a restriction endonuclease. 
B)  They can transiently base pair to complementary sticky ends on vector DNA. 
C)  They can covalently join to the vector in a reaction catalyzed by DNA gyrase. 
D)  All of the above 
A)  They are fragments with single strand extensions at only the 5ʹ end of a restriction endonuclease. 
B)  They can transiently base pair to complementary sticky ends on vector DNA. 
C)  They can covalently join to the vector in a reaction catalyzed by DNA gyrase. 
D)  All of the above

Question 18

Restriction fragment length polymorphisms are the result of .

Accepted Answer

A)  mutation 
B)  chromosome deletions 
C)  chromosome recombination 
D)  all of the above 
A)  mutation 
B)  chromosome deletions 
C)  chromosome recombination 
D)  all of the above

Question 19

How many recombinant clones must be present in a cDNA library to be 95% certain that the library contains one clone of a particular mRNA molecule representing 0.005% of the total mRNA?

Accepted Answer

A)  5.9 × 103  
B)  6 × 104  
C)  6 × 106  
D)  5 × 105 
A)  5.9 × 103  
B)  6 × 104  
C)  6 × 106  
D)  5 × 105

Question 20

Plasmids replicate autonomously within a host.

Accepted Answer

A) True 
 B)False

Exam 23: Recombinant Dna Technology

The oligodeoxythymidylate oligo dT fragment functions as a primer for synthesis of a complementary DNA strand by reverse transcriptase.

Shine-Dalgarno sequences are components of plasmid vectors which help to maximize the expression of inserted genes.

Changing factors such as the salt concentration and temperature can be used to vary the stringency of hybridization and allow for more or less mismatches in base pairing.

To discover whether a species has a gene that is similar but not identical to the cDNA of another species, one can

Which of the following is true concerning genomic libraries?

In general, when constructing a YAC library of genomic DNA the smaller the insert used the fewer clones are needed.

Bacterial artificial chromosomes BACs) require in order to successfully enter into bacterial cells.

Which is not an important feature of site-directed mutagenesis of cloned DNA?

Plasmids are usually introduced into bacterial cells by .

replicate in either prokaryotic or eukaryotic cells.

RFLP analysis is a very useful technique for genetic analysis and forensic studies, but it is not precise enough to distinguish genetic differences between highly related individuals such as siblings or parents and children.

Who won the Nobel Prize in 1993 for his work on site-directed mutagenesis?

RFLPs are very useful in determining paternity.

After ten cycles of PCR, by approximately what factor will the number of DNA molecules in a sample be increased? Assume maximum efficiency.

It can be difficult for bacteria to produce functional eukaryotic proteins even when all the components necessary for gene expression are present because .

Which of the following statements is true concerning sticky ends?

Restriction fragment length polymorphisms are the result of .

How many recombinant clones must be present in a cDNA library to be 95% certain that the library contains one clone of a particular mRNA molecule representing 0.005% of the total mRNA?

Plasmids replicate autonomously within a host.

Introduction to Biochemistry

Water

Amino Acids and the Primary Structures of Proteins

Proteins: Three-Dimensional Structure and Function

Properties of Enzymes

Mechanisms of Enzymes

Coenzymes and Vitamins

Carbohydrates

Lipids and Membranes

Introduction to Metabolism

Glycolysis

Gluconeogenesis, the Pentose Phosphate Pathway, and Glycogen Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Nucleic Acids

Dna Replication, Repair, and Recombination

Transcription and Rna Processing

Protein Synthesis

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