Question 1

What immersion oil is used most commonly in hematology?

Accepted Answer

A) type A 
B) type B 
C) type C 
D) type D 
A) type A 
B) type B 
C) type C 
D) type D B

Question 2

What should be adjusted if two overlapping images,instead of only one,are seen when looking through a microscope?

Accepted Answer

A) field diaphragm 
B) fine adjustment knob 
C) condenser knob 
D) interpupillary control 
A) field diaphragm 
B) fine adjustment knob 
C) condenser knob 
D) interpupillary control D

Question 3

What type of microscope helps in the identification of urine crystals?

Accepted Answer

A) compound brightfield 
B) phase contrast 
C) polarized light 
D) darkfield 
A) compound brightfield 
B) phase contrast 
C) polarized light 
D) darkfield C

Question 4

Match each lettered type of microscope to its clinical use.
-Routine differentials

Accepted Answer

A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield 
A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield

Question 5

Why is it not advisable to interchange one ocular lens with another from a different brand microscope?

Accepted Answer

A) The two ocular lenses in a microscope are optically matched with one another. 
B) The fitting for the ocular lenses varies, so they will not fit in another microscope. 
C) They cannot be removed from the microscope. 
D) They are fragile and are likely to break when removed from the microscope. 
A) The two ocular lenses in a microscope are optically matched with one another. 
B) The fitting for the ocular lenses varies, so they will not fit in another microscope. 
C) They cannot be removed from the microscope. 
D) They are fragile and are likely to break when removed from the microscope.

Question 6

Match each lettered type of microscope to its clinical use.
-Counting platelets manually

Accepted Answer

A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield 
A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield

Question 7

What converts for curvature in a field so that a flat field with uniform focus is seen?

Accepted Answer

A) achromatic objective lens 
B) plan-achromatic objective lens 
C) spherical correction ocular lens 
D) blue filter when placed above the condenser 
A) achromatic objective lens 
B) plan-achromatic objective lens 
C) spherical correction ocular lens 
D) blue filter when placed above the condenser

Question 8

Match each lettered type of microscope to its clinical use.
-Viewing spirochetes in microbiology

Accepted Answer

A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield 
A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield

Question 9

What is the most likely reason for cells on a hemocytometer appearing "fuzzy" when switching from low power to the *40 objective?

Accepted Answer

A) condenser not lowered enough 
B) hemocytometer not cleaned properly 
C) oil on the 40 x lens 
D) cells not allowed to settle 
A) condenser not lowered enough 
B) hemocytometer not cleaned properly 
C) oil on the 40 x lens 
D) cells not allowed to settle

Question 10

The ability to distinguish between two objects that are situated close to one another as separate entities is

Accepted Answer

A) resolution. 
B) parfocal. 
C) contrast. 
D) parcentric. 
A) resolution. 
B) parfocal. 
C) contrast. 
D) parcentric.

Question 11

What is the final magnification of an image if the ocular lens is 15* and the objective lens is 50*?

Accepted Answer

A) x150 
B) x500 
C) x750 
D) x1000 
A) x150 
B) x500 
C) x750 
D) x1000

Question 12

How should a microscope be carried?

Accepted Answer

A) both hands supporting the base 
B) both hands around the arm 
C) one hand around the arm and the other holding the oculars in place 
D) one hand under the base and the other around the arm 
A) both hands supporting the base 
B) both hands around the arm 
C) one hand around the arm and the other holding the oculars in place 
D) one hand under the base and the other around the arm

Question 13

What solution is best for cleaning lenses?

Accepted Answer

A) commercial glass-cleaning solution (e.g., Windex) 
B) water 
C) benzene 
D) xylol 
A) commercial glass-cleaning solution (e.g., Windex) 
B) water 
C) benzene 
D) xylol

Question 14

What can increase the resolution of an object under the microscope?

Accepted Answer

A) lowering the condenser 
B) increasing the combined magnification provided by the ocular and objective lenses 
C) enlarging the numeric aperture of the objective lens 
D) adjusting the focus controls 
A) lowering the condenser 
B) increasing the combined magnification provided by the ocular and objective lenses 
C) enlarging the numeric aperture of the objective lens 
D) adjusting the focus controls

Question 15

An image viewed under the microscope is clearly seen under both the 10x and 40x objectives,but it is not visible under *100.What is the most likely reason for this?

Accepted Answer

A) Oil is too thick on the slide. 
B) The slide is placed upside down on the stage. 
C) The slide has a coverslip on it. 
D) No oil is on the slide. 
A) Oil is too thick on the slide. 
B) The slide is placed upside down on the stage. 
C) The slide has a coverslip on it. 
D) No oil is on the slide.

Question 16

What is the purpose of the blue filter when using a tungsten-halogen microscope light?

Accepted Answer

A) eliminate blue color 
B) eliminate yellow color 
C) reduce light intensity 
D) filter out stray light 
A) eliminate blue color 
B) eliminate yellow color 
C) reduce light intensity 
D) filter out stray light

Question 17

Which of the following would be least likely to cause a "fuzzy" image on oil power?

Accepted Answer

A) using a plan-achromatic objective lens 
B) objective not screwed in tightly 
C) not enough oil on the slide when using oil immersion lens 
D) focus controls not adjusted 
A) using a plan-achromatic objective lens 
B) objective not screwed in tightly 
C) not enough oil on the slide when using oil immersion lens 
D) focus controls not adjusted

Question 18

Where should the condenser be located when using the *100 oil objective?

Accepted Answer

A) lowest position 
B) highest position 
C) middle position 
D) variable depending on amount of light desired 
A) lowest position 
B) highest position 
C) middle position 
D) variable depending on amount of light desired

Question 19

Match each lettered type of microscope to its clinical use.
-Identifying crystals in synovial fluid

Accepted Answer

A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield 
A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield

Question 20

The device used to assist in finding a cell of interest at a later time is called a

Accepted Answer

A) microlocator. 
B) micrometer. 
C) reticle. 
D) hemocytometer. 
A) microlocator. 
B) micrometer. 
C) reticle. 
D) hemocytometer.

Exam 4: Care and Use of the Microscope

What immersion oil is used most commonly in hematology?

What should be adjusted if two overlapping images,instead of only one,are seen when looking through a microscope?

What type of microscope helps in the identification of urine crystals?

Match each lettered type of microscope to its clinical use. -Routine differentials

Why is it not advisable to interchange one ocular lens with another from a different brand microscope?

Match each lettered type of microscope to its clinical use. -Counting platelets manually

What converts for curvature in a field so that a flat field with uniform focus is seen?

Match each lettered type of microscope to its clinical use. -Viewing spirochetes in microbiology

What is the most likely reason for cells on a hemocytometer appearing "fuzzy" when switching from low power to the *40 objective?

The ability to distinguish between two objects that are situated close to one another as separate entities is

What is the final magnification of an image if the ocular lens is 15* and the objective lens is 50*?

How should a microscope be carried?

What solution is best for cleaning lenses?

What can increase the resolution of an object under the microscope?

An image viewed under the microscope is clearly seen under both the 10x and 40x objectives,but it is not visible under *100.What is the most likely reason for this?

What is the purpose of the blue filter when using a tungsten-halogen microscope light?

Which of the following would be least likely to cause a "fuzzy" image on oil power?

Where should the condenser be located when using the *100 oil objective?

Match each lettered type of microscope to its clinical use. -Identifying crystals in synovial fluid

The device used to assist in finding a cell of interest at a later time is called a

An Overview of Clinical Laboratory Hematology

Safety in the Hematology Laboratory

Blood Specimen Collection

Quality Assurance in Hematology and Hemostasis Testing

Cellular Structure and Function

Hematopoiesis

Erythrocyte Production and Destruction

Erythrocyte Metabolism and Membrane Structure and Function

Hemoglobin Metabolism

Iron Kinetics and Laboratory Assessment

Leukocyte Development, Kinetics, and Functions

Platelet Production, Structure, and Function

Manual, Semiautomated and Point-of-Care Testing in Hematology

Automated Blood Cell Analysis

Examination of the Peripheral Blood Film and Correlation with the Complete Blood Count

Bone Marrow Examination

Body Fluid Analysis in the Hematology Laboratory

Anemias: Red Blood Cell Morphology and Approach to Diagnosis

Disorders of Iron Kinetics and Heme Metabolism

Anemias Caused by Defects of DNA Metabolism

Bone Marrow Failure

Introduction to Increased Destruction of Erythrocytes

Intrinsic Defects Leading to Increased Erythrocyte Destruction

Extrinsic Defects Leading to Increased Erythrocyte Destruction Nonimmune Causes

Extrinsic Defects Leading to Increased Erythrocyte Destruction Immune Causes

Hemoglobinopathies (Structural Defects in Hemoglobin)

Thalassemias

Nonmalignant Leukocyte Disorders

Cytogenetics

Molecular Diagnostics in Hematopathology

Flow Cytometric Analysis in Hematologic Disorders

Myeloproliferative Neoplasms

Myelodysplastic Syndromes

Acute Leukemias

Mature Lymphoid Neoplasms

Normal Hemostasis and Coagulation

Hemorrhagic Disorders and Laboratory Assessment

Thrombotic Disorders and Laboratory Assessment

Thrombocytopenia and Thrombocytosis

Qualitative Disorders of Platelets and Vasculature

Laboratory Evaluation of Hemostasis

Antithrombotic Therapies and their Laboratory Assessment

Hemostasis and Coagulation Instrumentation

Pediatric and Geriatric Hematology and Hemostasis

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