Question 1

A compound microscope

Accepted Answer

A)  has two eyepieces 
B)  has a total magnification of 5,000X 
C)  has only fine adjustment and no coarse adjustment 
D)  has more than one lens 
A)  has two eyepieces 
B)  has a total magnification of 5,000X 
C)  has only fine adjustment and no coarse adjustment 
D)  has more than one lens

Question 2

A parfocal microscope

Accepted Answer

A)  has more than one source of illumination 
B)  has both coarse and fine focusing adjustment 
C)  accentuates small differences in the refractive index of intracellular structures 
D)  allows for specimens to remain in focus when changing between magnification 
A)  has more than one source of illumination 
B)  has both coarse and fine focusing adjustment 
C)  accentuates small differences in the refractive index of intracellular structures 
D)  allows for specimens to remain in focus when changing between magnification

Question 3

What is true about the index of refraction?

Accepted Answer

A)  if light rays are taken up by the object then it has a high index of refraction 
B)  it measures the frequency of the light as it reflects from a material 
C)  oil immersion lenses increase the problem of refraction 
D)  light will bend as it passes through two substances with different indices of refraction 
A)  if light rays are taken up by the object then it has a high index of refraction 
B)  it measures the frequency of the light as it reflects from a material 
C)  oil immersion lenses increase the problem of refraction 
D)  light will bend as it passes through two substances with different indices of refraction

Question 4

Which stain would be the best choice for detecting mycobacterium (the bacteria responsible for tuberculosis and leprosy)?

Accepted Answer

A)  simple stain 
B)  endospore stain 
C)  acid-fast stain 
D)  Gram stain 
A)  simple stain 
B)  endospore stain 
C)  acid-fast stain 
D)  Gram stain

Question 5

The lens closest to the slide during a microscopic examination is the

Accepted Answer

A)  ocular 
B)  objective 
C)  condenser 
D)  compound 
A)  ocular 
B)  objective 
C)  condenser 
D)  compound

Question 6

The formula for resolving power of a lens is l/2NA (wavelength /2 x numerical aperture). What does this say about resolving power?

Accepted Answer

A)  the smaller the wavelength the greater the resolving power of the lens 
B)  it is not related to the lens' numerical aperture 
C)  we can not precisely calculate the resolving power of a lens 
D)  a larger resolving power is indicative of a better lens 
A)  the smaller the wavelength the greater the resolving power of the lens 
B)  it is not related to the lens' numerical aperture 
C)  we can not precisely calculate the resolving power of a lens 
D)  a larger resolving power is indicative of a better lens

Question 7

Most light microscopes contain a/n ________ that converges the light beam so that it passes through the specimen

Accepted Answer

A)  objective lens 
B)  iris diaphragm 
C)  mechanical stage 
D)  condenser 
A)  objective lens 
B)  iris diaphragm 
C)  mechanical stage 
D)  condenser

Question 8

Which of the following statements about resolution is true?

Accepted Answer

A)  refers to the ability of a lens to distinguish adjacent objects 
B)  with regard to light, means the same thing as wavelength 
C)  refers to a microscope's ability to magnify objects 
D)  is equal to the distance between two adjacent crests of a wave 
A)  refers to the ability of a lens to distinguish adjacent objects 
B)  with regard to light, means the same thing as wavelength 
C)  refers to a microscope's ability to magnify objects 
D)  is equal to the distance between two adjacent crests of a wave

Question 9

Which of the following statements about Leeuwenhoek's microscopes is false?

Accepted Answer

A)  Leeuwenhoek kept his technique secret 
B)  they magnified objects 100 to 300 times 
C)  for each specimen a new microscope had to be made 
D)  they were able to reveal very fine details of bacteria 
A)  Leeuwenhoek kept his technique secret 
B)  they magnified objects 100 to 300 times 
C)  for each specimen a new microscope had to be made 
D)  they were able to reveal very fine details of bacteria

Question 10

Bacteria capsules can best be visualized by _______ staining.

Accepted Answer

A)  flagellar 
B)  crystal violet 
C)  negative 
D)  mordant 
A)  flagellar 
B)  crystal violet 
C)  negative 
D)  mordant

Question 11

A microscope in which light rays pass directly through a specimen is a ________ microscope.

Accepted Answer

A)  bright field 
B)  dark field 
C)  phase-contrast 
D)  Nomarski 
A)  bright field 
B)  dark field 
C)  phase-contrast 
D)  Nomarski

Question 12

Colored photos of electron micrographs

Accepted Answer

A)  reflect the color of the specimen before it was frozen 
B)  are false color added on during image preparation 
C)  reflect the color of the specimen after it was frozen 
D)  are always in pastel shades 
A)  reflect the color of the specimen before it was frozen 
B)  are false color added on during image preparation 
C)  reflect the color of the specimen after it was frozen 
D)  are always in pastel shades

Question 13

Atomic force microscope

Accepted Answer

A)  allows 3 dimensional imaging and measurement of structures as small as nucleotides in DNA 
B)  is not yet capable of measuring small forces 
C)  involves ultraviolet light exciting molecules so that they release light of a longer wavelength 
D)  has a special condenser and objective lens 
A)  allows 3 dimensional imaging and measurement of structures as small as nucleotides in DNA 
B)  is not yet capable of measuring small forces 
C)  involves ultraviolet light exciting molecules so that they release light of a longer wavelength 
D)  has a special condenser and objective lens

Question 14

Light of ________ wavelength typically will result in ________ resolution.

Accepted Answer

A)  longer, better 
B)  shorter, better 
C)  any, poor 
D)  shorter, worse 
A)  longer, better 
B)  shorter, better 
C)  any, poor 
D)  shorter, worse

Question 15

A compound light microscope can generally see objects no smaller than

Accepted Answer

A)  a ribosome 
B)  a large protozoa 
C)  a small bacterium 
D)  a typical virus 
A)  a ribosome 
B)  a large protozoa 
C)  a small bacterium 
D)  a typical virus

Question 16

In a properly executed Gram stain,Gram positive organisms appear ________ while Gram negative organisms appear ________.

Accepted Answer

A)  pink, clear 
B)  pink, purple 
C)  purple, pink 
D)  purple, blue 
A)  pink, clear 
B)  pink, purple 
C)  purple, pink 
D)  purple, blue

Question 17

Which of the following is not a differential stain?

Accepted Answer

A)  Gram stain 
B)  Schaeffer-Fulton 
C)  acid-fast stain 
D)  flagellar stain 
A)  Gram stain 
B)  Schaeffer-Fulton 
C)  acid-fast stain 
D)  flagellar stain

Question 18

What statement about microscopy and staining techniques is false?

Accepted Answer

A)  many species look identical under the microscope 
B)  staining and microscopic examination are usually all that is need to identify a microorganism. 
C)  microscopes are of little use unless the specimens are prepared properly 
D)  degree of contrast is equally important as resolution and magnification 
A)  many species look identical under the microscope 
B)  staining and microscopic examination are usually all that is need to identify a microorganism. 
C)  microscopes are of little use unless the specimens are prepared properly 
D)  degree of contrast is equally important as resolution and magnification

Question 19

If the step involving iodine were left out of a Gram stain,which of the following would best describe the results?

Accepted Answer

A)  Gram negative cells would look Gram positive 
B)  Gram positive cells would look Gram negative 
C)  All rods would be pink, all cocci purple 
D)  All cells would be purple 
A)  Gram negative cells would look Gram positive 
B)  Gram positive cells would look Gram negative 
C)  All rods would be pink, all cocci purple 
D)  All cells would be purple

Question 20

Electron microscopes

Accepted Answer

A)  that are scanning have better resolution than those that are transmission 
B)  are much more expensive and take up more space than light microscopes 
C)  can use the same preparations of specimens that have been prepared for viewing with a light microscope 
D)  have a resolving power approximately 10 times better than the best light microscope 
A)  that are scanning have better resolution than those that are transmission 
B)  are much more expensive and take up more space than light microscopes 
C)  can use the same preparations of specimens that have been prepared for viewing with a light microscope 
D)  have a resolving power approximately 10 times better than the best light microscope

Exam 3: Microscopy and Staining

A compound microscope

A parfocal microscope

What is true about the index of refraction?

Which stain would be the best choice for detecting mycobacterium (the bacteria responsible for tuberculosis and leprosy)?

The lens closest to the slide during a microscopic examination is the

The formula for resolving power of a lens is l/2NA (wavelength /2 x numerical aperture). What does this say about resolving power?

Most light microscopes contain a/n ________ that converges the light beam so that it passes through the specimen

Which of the following statements about resolution is true?

Which of the following statements about Leeuwenhoek's microscopes is false?

Bacteria capsules can best be visualized by _______ staining.

A microscope in which light rays pass directly through a specimen is a ________ microscope.

Colored photos of electron micrographs

Atomic force microscope

Light of ________ wavelength typically will result in ________ resolution.

A compound light microscope can generally see objects no smaller than

In a properly executed Gram stain,Gram positive organisms appear ________ while Gram negative organisms appear ________.

Which of the following is not a differential stain?

What statement about microscopy and staining techniques is false?

If the step involving iodine were left out of a Gram stain,which of the following would best describe the results?

Electron microscopes

Scope and History of Microbiology

Fundamentals of Chemistry

Characteristics of Prokaryotic and Eukaryotic Cells

Essential Concepts of Metabolism

Growth and Culturing of Bacteria

Microbial Genetics

Gene Transfer and Genetic Engineering

An Introduction to Taxonomy: the Bacteria

Viruses

Eukaryotic Microorganisms and Parasites

Sterilization and Disinfection

Antimicrobial Therapy

Host-Microbe Relationships and Disease Processes

Epidemiology and Nosocomial Infections

Innate Host Defenses

Immunology I: Basic Principles of Adaptive Immunity and Immunization

Immunolgy Ii: Immunological Disorders and Tests

Diseases of the Skin and Eyes; Wounds and Bites

Urogenital and Sexually Transmitted Diseases

Diseases of the Respiratory System

Oral and Gastrointestinal Diseases

Cardiovascular, lymphatic and Systemic Disease

Diseases of the Nervous System

Environmental Microbiology

Applied Microbiology

Filters

Light of wavelength typically will result in resolution.

In a properly executed Gram stain,Gram positive organisms appear while Gram negative organisms appear .