Question 1

Which statement best describes the key difference between the "enhanceosome" and "hit and run" models for transcriptional activation?

Accepted Answer

A)  The "enhanceosome" model requires the involvement of both promoter proximal elements and the core promoter, whereas the "hit and run" model only involves promoter proximal elements. 
B)  The "enhanceosome" model requires that a stable transcriptional activation complex assembles in an ordered fashion, whereas the "hit and run" model suggests that such complexes are formed stochastically. 
C)  The "enhanceosome" model requires synergy between multiple transcriptional (co)activators, whereas the "hit and run" model allows that each transcriptional regulatory protein may function independently. 
D)  The "enhanceosome" model only applies to genes that undergo developmental regulation, whereas the "hit and run" model only applies to genes that undergo dynamic regulation even in a terminally differentiated cell. 
A)  The "enhanceosome" model requires the involvement of both promoter proximal elements and the core promoter, whereas the "hit and run" model only involves promoter proximal elements. 
B)  The "enhanceosome" model requires that a stable transcriptional activation complex assembles in an ordered fashion, whereas the "hit and run" model suggests that such complexes are formed stochastically. 
C)  The "enhanceosome" model requires synergy between multiple transcriptional (co)activators, whereas the "hit and run" model allows that each transcriptional regulatory protein may function independently. 
D)  The "enhanceosome" model only applies to genes that undergo developmental regulation, whereas the "hit and run" model only applies to genes that undergo dynamic regulation even in a terminally differentiated cell. B

Question 2

Which is not a common DNA binding motif?

Accepted Answer

A)  chromodomain 
B)  zinc finger 
C)  helix-turn-helix 
D)  basic helix-loop-helix 
A)  chromodomain 
B)  zinc finger 
C)  helix-turn-helix 
D)  basic helix-loop-helix A

Question 3

Which of the following statements about silencers is correct?

Accepted Answer

A)  They contain a consensus sequence called a TATA box. 
B)  They are found in a variety of locations and are functional in any orientation. 
C)  They are located only in introns. 
D)  They are located only in 5?-flanking regions. 
A)  They contain a consensus sequence called a TATA box. 
B)  They are found in a variety of locations and are functional in any orientation. 
C)  They are located only in introns. 
D)  They are located only in 5?-flanking regions. B

Question 4

Describe the G-less cassette transcription assay that led to the discovery of Mediator. Include a graph illustrating the effect of Mediator on activated versus basal transcription

Accepted Answer

The G-less cassette transcription assay

Question 5

Could the Polycomb-group protein complexes PRC1 and PRC2 be considered co-repressors? Why or why not?

Accepted Answer

Formally, PRC1 and PRC2 could only be co

Question 6

Which method was most instrumental in the development of the current model for transcript elongation by RNA Polymerase II?

Accepted Answer

A)  in vitro run-off assay 
B)  fluorescence recovery after photobleaching (FRAP) 
C)  X-ray crystallography 
D)  electromobility shift assay (EMS
A)  
A)  in vitro run-off assay 
B)  fluorescence recovery after photobleaching (FRAP) 
C)  X-ray crystallography 
D)  electromobility shift assay (EMS
A)

Question 7

Fundamentally, what makes one cell different from another in a multicellular eukaryote?

Accepted Answer

A)  The different cells contain different sets of enhancers and promoter-proximal elements. 
B)  The different cells contain different sets of transcription factors. 
C)  The different cells contain different sets of cell-type-specific genes. 
A)  The different cells contain different sets of enhancers and promoter-proximal elements. 
B)  The different cells contain different sets of transcription factors. 
C)  The different cells contain different sets of cell-type-specific genes.

Question 8

Compare and contrast the "enhanceosome" and the "hit and run" model for transcription complex assembly.

Accepted Answer

The "enhanceosome" and the "hit and run"

Question 9

Patients with "Hispanic thalassemia" have a deletion of the ________ of the beta-globin gene cluster that results in silencing of the genes.

Accepted Answer

A)  locus control region 
B)  coding region 
C)  matrix attachment region 
D)  promoter 
A)  locus control region 
B)  coding region 
C)  matrix attachment region 
D)  promoter

Question 10

List in order the proteins that assemble to form an RNA polymerase II preinitiation complex.

Accepted Answer

The RNA polymerase II preinitiation comp

Question 11

Which is the correct order of recruitment of transcriptional regulatory proteins to a gene promoter?

Accepted Answer

A)  SWI/SNF, histone acetyltransferase (HAT) complex, preinitiation complex 
B)  HAT complex, SWI/SNF, preinitiation complex 
C)  preinitiation complex, SWI/SNF, HAT complex 
D)  the order of recruitment is gene-specific. 
A)  SWI/SNF, histone acetyltransferase (HAT) complex, preinitiation complex 
B)  HAT complex, SWI/SNF, preinitiation complex 
C)  preinitiation complex, SWI/SNF, HAT complex 
D)  the order of recruitment is gene-specific.

Question 12

In an in vitro transcription assay, a G-less cassette could be used to do which of the following?

Accepted Answer

A)  generate an RNA product of defined length 
B)  pause RNA polymerase at a defined site in a template 
C)  induce RNA polymerase backtracking 
D)  A and B 
A)  generate an RNA product of defined length 
B)  pause RNA polymerase at a defined site in a template 
C)  induce RNA polymerase backtracking 
D)  A and B

Question 13

You suspect that a sequence upstream of a transcriptional start site is acting as an enhancer and not as a promoter. Describe an experiment you would run to test your hypothesis. Predict the results.

Accepted Answer

Promoter elements increase the frequency

Question 14

Which regulatory sequences must maintain their orientation with respect to the gene transcription start site to function?

Accepted Answer

A)  promoter and silencer 
B)  promoter and enhancer 
C)  promoter and locus control region 
D)  silencer and enhancer 
A)  promoter and silencer 
B)  promoter and enhancer 
C)  promoter and locus control region 
D)  silencer and enhancer

Question 15

Do coactivators and corepressors bind DNA directly? Explain your

Accepted Answer

Coactivators and corepressors are types

Question 16

The primary difference between an enhancer and a promoter-proximal element is that:

Accepted Answer

A)  Enhancers are transcription factors; promoter-proximal elements are DNA sequences. 
B)  Enhancers enhance transcription; promoter proximal-elements inhibit transcription. 
C)  Enhancers are part of the core promoter; promoter-proximal elements are regulatory sequences distinct from the core promoter. 
D)  Enhancers are at considerable distances from the core promoter; promoter-proximal elements are close to the core promoter. 
A)  Enhancers are transcription factors; promoter-proximal elements are DNA sequences. 
B)  Enhancers enhance transcription; promoter proximal-elements inhibit transcription. 
C)  Enhancers are part of the core promoter; promoter-proximal elements are regulatory sequences distinct from the core promoter. 
D)  Enhancers are at considerable distances from the core promoter; promoter-proximal elements are close to the core promoter.

Question 17

Which protein or protein complex helps RNA polymerase to traverse nucleosomes?

Accepted Answer

A)  FACT 
B)  SWI/SNF 
C)  Sonic hedgehog 
D)  Polycomb 
A)  FACT 
B)  SWI/SNF 
C)  Sonic hedgehog 
D)  Polycomb

Question 18

Imagine you're assaying the DNase I sensitivity of the promoter regions of the beta-globin and vitellogenin genes in chicken liver, instead of in chick embryo erythroblasts. Show sample results from assay.

Accepted Answer

To assay the DNase I sensitivity of the

Question 19

Which of the following is not true of Hox family genes?

Accepted Answer

A)  Each Hox gene has a 180 base pair sequence called the homeobox. 
B)  In both fruit flies and mammals, the order of the Hox genes on the chromosomecorrelates with where they are expressed in embryos. 
C)  In fruit flies the order of Hox genes on the chromosome correlates with where they are expressed in embryos; in mammals there is no correlation. 
D)  The expression of the Hox genes is sequential, moving in order along the chromosome. 
A)  Each Hox gene has a 180 base pair sequence called the homeobox. 
B)  In both fruit flies and mammals, the order of the Hox genes on the chromosomecorrelates with where they are expressed in embryos. 
C)  In fruit flies the order of Hox genes on the chromosome correlates with where they are expressed in embryos; in mammals there is no correlation. 
D)  The expression of the Hox genes is sequential, moving in order along the chromosome.

Question 20

Which of following events is not true about the nuclear import of proteins?

Accepted Answer

A)  The nuclear localization sequence of the protein to be imported binds to importin. 
B)  The nuclear localization sequence of the protein is removed once the protein enters the nucleus. 
C)  The energy for import is provided by the small GTPase Ran. 
D)  Import may occur against a concentration gradient. 
A)  The nuclear localization sequence of the protein to be imported binds to importin. 
B)  The nuclear localization sequence of the protein is removed once the protein enters the nucleus. 
C)  The energy for import is provided by the small GTPase Ran. 
D)  Import may occur against a concentration gradient.

Which statement best describes the key difference between the "enhanceosome" and "hit and run" models for transcriptional activation?

Which is not a common DNA binding motif?

Which of the following statements about silencers is correct?

Describe the G-less cassette transcription assay that led to the discovery of Mediator. Include a graph illustrating the effect of Mediator on activated versus basal transcription

Could the Polycomb-group protein complexes PRC1 and PRC2 be considered co-repressors? Why or why not?

Which method was most instrumental in the development of the current model for transcript elongation by RNA Polymerase II?

Fundamentally, what makes one cell different from another in a multicellular eukaryote?

Compare and contrast the "enhanceosome" and the "hit and run" model for transcription complex assembly.

Patients with "Hispanic thalassemia" have a deletion of the ________ of the beta-globin gene cluster that results in silencing of the genes.

List in order the proteins that assemble to form an RNA polymerase II preinitiation complex.

Which is the correct order of recruitment of transcriptional regulatory proteins to a gene promoter?

In an in vitro transcription assay, a G-less cassette could be used to do which of the following?

You suspect that a sequence upstream of a transcriptional start site is acting as an enhancer and not as a promoter. Describe an experiment you would run to test your hypothesis. Predict the results.

Which regulatory sequences must maintain their orientation with respect to the gene transcription start site to function?

Do coactivators and corepressors bind DNA directly? Explain your

The primary difference between an enhancer and a promoter-proximal element is that:

Which protein or protein complex helps RNA polymerase to traverse nucleosomes?

Imagine you're assaying the DNase I sensitivity of the promoter regions of the beta-globin and vitellogenin genes in chicken liver, instead of in chick embryo erythroblasts. Show sample results from assay.

Which of the following is not true of Hox family genes?

Which of following events is not true about the nuclear import of proteins?

The Beginnings of Molecular Biology

The Structure of DNA

The Versatility of RNA

Protein Structure and Folding

Genome Organization and Evolution

DNA Replication and Telomere Maintenance

DNA Repair Pathways

Recombinant Dna Technology and Molecular Cloning

Tools for Analyzing Gene Expression

Transcription in Bacteria

Epigenetic Mechanisms of Gene Regulation

RNA Processing and Post-Transcriptional Gene Regulation

The Mechanism of Translation

Genetically Modified Organisms: Use in Basic and Applied Research

Genome Analysis: DNA Typing, Genomics, and Beyond

Medical Molecular Biology

Filters

Exam 11: Transcription in Eukaryotes

Which statement best describes the key difference between the "enhanceosome" and "hit and run" models for transcriptional activation?

Which is not a common DNA binding motif?

Which of the following statements about silencers is correct?

Describe the G-less cassette transcription assay that led to the discovery of Mediator. Include a graph illustrating the effect of Mediator on activated versus basal transcription

Could the Polycomb-group protein complexes PRC1 and PRC2 be considered co-repressors? Why or why not?

Which method was most instrumental in the development of the current model for transcript elongation by RNA Polymerase II?

Fundamentally, what makes one cell different from another in a multicellular eukaryote?

Compare and contrast the "enhanceosome" and the "hit and run" model for transcription complex assembly.

Patients with "Hispanic thalassemia" have a deletion of the ________ of the beta-globin gene cluster that results in silencing of the genes.

List in order the proteins that assemble to form an RNA polymerase II preinitiation complex.

Which is the correct order of recruitment of transcriptional regulatory proteins to a gene promoter?

In an in vitro transcription assay, a G-less cassette could be used to do which of the following?

You suspect that a sequence upstream of a transcriptional start site is acting as an enhancer and not as a promoter. Describe an experiment you would run to test your hypothesis. Predict the results.

Which regulatory sequences must maintain their orientation with respect to the gene transcription start site to function?

Do coactivators and corepressors bind DNA directly? Explain your

The primary difference between an enhancer and a promoter-proximal element is that:

Which protein or protein complex helps RNA polymerase to traverse nucleosomes?

Imagine you're assaying the DNase I sensitivity of the promoter regions of the beta-globin and vitellogenin genes in chicken liver, instead of in chick embryo erythroblasts. Show sample results from assay.

Which of the following is not true of Hox family genes?

Which of following events is not true about the nuclear import of proteins?

The Beginnings of Molecular Biology

The Structure of DNA

The Versatility of RNA

Protein Structure and Folding

Genome Organization and Evolution

DNA Replication and Telomere Maintenance

DNA Repair Pathways

Recombinant Dna Technology and Molecular Cloning

Tools for Analyzing Gene Expression

Transcription in Bacteria

Epigenetic Mechanisms of Gene Regulation

RNA Processing and Post-Transcriptional Gene Regulation

The Mechanism of Translation

Genetically Modified Organisms: Use in Basic and Applied Research

Genome Analysis: DNA Typing, Genomics, and Beyond

Medical Molecular Biology

Filters