Question 1

In practice, the unit of length used for DNA is the kilobase pair, which corresponds to

Accepted Answer

A)  10 base pairs 
B)  100 base pairs 
C)  1000 base pairs 
D)  1,000,000 base pairs 
A)  10 base pairs 
B)  100 base pairs 
C)  1000 base pairs 
D)  1,000,000 base pairs C

Question 2

Most sequence-specific DNA binding proteins interact with

Accepted Answer

A)  the minor groove of Z-DNA 
B)  the major groove of B-DNA 
C)  the backbone of A-DNA 
D)  locally unwound DNA 
A)  the minor groove of Z-DNA 
B)  the major groove of B-DNA 
C)  the backbone of A-DNA 
D)  locally unwound DNA B

Question 3

You have isolated a DNA molecule that is 12,200 base pairs in length. How many kilobase pairs is the DNA molecule?

Accepted Answer

A DNA molecule that is 12,200 base pairs in length can be expressed in kilobase pairs (kb) by dividing the number of base pairs by 1,000, since "kilo-" denotes a thousandfold.

So, 12,200 base pairs ÷ 1,000 = 12.2 kilobase pairs.

Therefore, the DNA molecule is 12.2 kb in length.

Question 4

A Hoogsteen base pair differs from a Watson-Crick base pair by

Accepted Answer

A)  an altered pattern of hydrogen bonding in the Hoogsteen AT pair 
B)  the Hoogsteen GC pair only forms two hydrogen bonds 
C)  Hoogsteen GC pairs are not stable at neutral pH 
D)  all of the above 
A)  an altered pattern of hydrogen bonding in the Hoogsteen AT pair 
B)  the Hoogsteen GC pair only forms two hydrogen bonds 
C)  Hoogsteen GC pairs are not stable at neutral pH 
D)  all of the above

Question 5

Explain how "hydrophobic bonding" stabilizes double-stranded DNA in aqueous solution.

Accepted Answer

Nitrogenous bases contain nonpolar rings

Question 6

Lowering the salt concentration of a DNA solution

Accepted Answer

A)  promotes renaturation. 
B)  promotes denaturation. 
C)  promotes formation of hydrogen bonds. 
D)  removes cations that shield the positive charges on the two strands from each other. 
A)  promotes renaturation. 
B)  promotes denaturation. 
C)  promotes formation of hydrogen bonds. 
D)  removes cations that shield the positive charges on the two strands from each other.

Question 7

Which statement is not true about DNA supercoiling?

Accepted Answer

A)  Negative supercoiling puts energy into DNA. 
B)  In bacteriophages, negative supercoiling is associated with decreased activity in replication and transcription. 
C)  Positive supercoiling occurs ahead of replication forks and transcription complexes. 
D)  Most DNA within both prokaryotic and eukaryotic cells exists in the negative supercoiled state. 
A)  Negative supercoiling puts energy into DNA. 
B)  In bacteriophages, negative supercoiling is associated with decreased activity in replication and transcription. 
C)  Positive supercoiling occurs ahead of replication forks and transcription complexes. 
D)  Most DNA within both prokaryotic and eukaryotic cells exists in the negative supercoiled state.

Question 8

In the two common Watson-Crick base pairs of DNA

Accepted Answer

A)  Adenine (
A)  is joined to thymine (T) by three hydrogen bonds, while guanine (G) is joined to cytosine (
C)  by two hydrogen bonds. 
B)  Adenine (
A)  is joined to thymine (T) by two hydrogen bonds, while guanine (G) is joined to cytosine (
C)  by three hydrogen bonds. 
C)  Adenine (
A)  is joined to cytosine (
C)  by three hydrogen bonds, while guanine (G) is joined to thymine (T) by two hydrogen bonds. 
D)  Adenine (
A)  is joined to cytosine (
C)  by two hydrogen bonds, while guanine (G) is joined to thymine (T) by three hydrogen bonds. 
A)  Adenine (
A)  is joined to thymine (T) by three hydrogen bonds, while guanine (G) is joined to cytosine (
C)  by two hydrogen bonds. 
B)  Adenine (
A)  is joined to thymine (T) by two hydrogen bonds, while guanine (G) is joined to cytosine (
C)  by three hydrogen bonds. 
C)  Adenine (
A)  is joined to cytosine (
C)  by three hydrogen bonds, while guanine (G) is joined to thymine (T) by two hydrogen bonds. 
D)  Adenine (
A)  is joined to cytosine (
C)  by two hydrogen bonds, while guanine (G) is joined to thymine (T) by three hydrogen bonds.

Question 9

Edwin Chargaff found that the base composition of DNA, defined as the "percent G+C,"

Accepted Answer

A)  differs among species 
B)  is constant in all cells of an organism within a species 
C)  is the same among species 
D)  both A and B 
A)  differs among species 
B)  is constant in all cells of an organism within a species 
C)  is the same among species 
D)  both A and B

Question 10

Watson and Crick noted that DNA's structure is interesting because it suggested a possible copying mechanism. What about DNA's structure facilitates copying?

Accepted Answer

A)  It has the same number of purines and pyrimidines. 
B)  The nitrogenous bases are located on the inside of the double helix. 
C)  The strands of the double helix are complementary. 
D)  DNA always goes from 5' to 3'. 
A)  It has the same number of purines and pyrimidines. 
B)  The nitrogenous bases are located on the inside of the double helix. 
C)  The strands of the double helix are complementary. 
D)  DNA always goes from 5' to 3'.

Question 11

Supercoiling is a form of DNA

Accepted Answer

A)  primary structure 
B)  secondary structure 
C)  tertiary structure 
D)  quaternary structure 
A)  primary structure 
B)  secondary structure 
C)  tertiary structure 
D)  quaternary structure

Question 12

DNA-mediated heredity derives from which part of its nucleotide building blocks?

Accepted Answer

A)  the sugars 
B)  the phosphates 
C)  the nitrogenous bases 
D)  none of the above 
A)  the sugars 
B)  the phosphates 
C)  the nitrogenous bases 
D)  none of the above

Question 13

The __________________ carries a message (the base sequence of DNA) in a form that can be read by DNA-binding proteins; the bases form __________________ with the side chains of amino acids of proteins.

Accepted Answer

A)  major groove, hydrogen bonds 
B)  minor groove, hydrogen bonds 
C)  major groove, peptide bonds 
D)  minor groove, peptide bonds 
A)  major groove, hydrogen bonds 
B)  minor groove, hydrogen bonds 
C)  major groove, peptide bonds 
D)  minor groove, peptide bonds

Question 14

Use a drawing to illustrate the principles of denaturation, renaturation, and nucleic acid hybridization.

Accepted Answer

Denaturation, renaturation, and nucleic

Question 15

Explain what is meant by the following descriptions of the DNA double helix: "polarity in each strand," and "the two strands are antiparallel."

Accepted Answer

The term "polarity in each strand" refer

Question 16

DNA loop domains

Accepted Answer

A)  are stabilized by proteins at loop junctions 
B)  join B-DNA and Z-DNA segments 
C)  are also known as DNA cruciforms 
D)  form spontaneously 
A)  are stabilized by proteins at loop junctions 
B)  join B-DNA and Z-DNA segments 
C)  are also known as DNA cruciforms 
D)  form spontaneously

Question 17

Draw a typical DNA denaturation curve. Label the axes and point out the melting temperature (Tm). Indicate at what points all of the DNA is double-stranded and all of the DNA is single-stranded.

Accepted Answer

A typical DNA denaturation curve is a gr

Question 18

Briefly describe the two main types of interactions that provide chemical stability to the DNA double helix.

Accepted Answer

The DNA double helix achieves its chemic

Question 19

Friedreich's ataxia is a rare inherited neurological disease caused by a

Accepted Answer

A)  trinucleotide repeat expansion in the first intron of the Friedrich's ataxia gene 
B)  formation of triple helix DNA in the Friedrich's ataxia gene 
C)  cruciform structures in the frataxin protein 
D)  both A and B 
A)  trinucleotide repeat expansion in the first intron of the Friedrich's ataxia gene 
B)  formation of triple helix DNA in the Friedrich's ataxia gene 
C)  cruciform structures in the frataxin protein 
D)  both A and B

Question 20

Which statement about B-DNA is not true?

Accepted Answer

A)  B-DNA occurs under conditions of relatively low humidity and relatively low salt. 
B)  B-DNA forms a right-handed helix. 
C)  B-DNA has 10.5 bases per turn of the helix. 
D)  B-DNA has a wide major groove of moderate depth. 
A)  B-DNA occurs under conditions of relatively low humidity and relatively low salt. 
B)  B-DNA forms a right-handed helix. 
C)  B-DNA has 10.5 bases per turn of the helix. 
D)  B-DNA has a wide major groove of moderate depth.

In practice, the unit of length used for DNA is the kilobase pair, which corresponds to

Most sequence-specific DNA binding proteins interact with

You have isolated a DNA molecule that is 12,200 base pairs in length. How many kilobase pairs is the DNA molecule?

A Hoogsteen base pair differs from a Watson-Crick base pair by

Explain how "hydrophobic bonding" stabilizes double-stranded DNA in aqueous solution.

Lowering the salt concentration of a DNA solution

Which statement is not true about DNA supercoiling?

In the two common Watson-Crick base pairs of DNA

Edwin Chargaff found that the base composition of DNA, defined as the "percent G+C,"

Watson and Crick noted that DNA's structure is interesting because it suggested a possible copying mechanism. What about DNA's structure facilitates copying?

Supercoiling is a form of DNA

DNA-mediated heredity derives from which part of its nucleotide building blocks?

The carries a message (the base sequence of DNA) in a form that can be read by DNA-binding proteins; the bases form with the side chains of amino acids of proteins.

Use a drawing to illustrate the principles of denaturation, renaturation, and nucleic acid hybridization.

Explain what is meant by the following descriptions of the DNA double helix: "polarity in each strand," and "the two strands are antiparallel."

DNA loop domains

Draw a typical DNA denaturation curve. Label the axes and point out the melting temperature (T_m). Indicate at what points all of the DNA is double-stranded and all of the DNA is single-stranded.

Briefly describe the two main types of interactions that provide chemical stability to the DNA double helix.

Friedreich's ataxia is a rare inherited neurological disease caused by a

Which statement about B-DNA is not true?

The Beginnings of Molecular Biology

The Versatility of RNA

Protein Structure and Folding

Genome Organization and Evolution

DNA Replication and Telomere Maintenance

DNA Repair Pathways

Recombinant Dna Technology and Molecular Cloning

Tools for Analyzing Gene Expression

Transcription in Bacteria

Transcription in Eukaryotes

Epigenetic Mechanisms of Gene Regulation

RNA Processing and Post-Transcriptional Gene Regulation

The Mechanism of Translation

Genetically Modified Organisms: Use in Basic and Applied Research

Genome Analysis: DNA Typing, Genomics, and Beyond

Medical Molecular Biology

Filters

Exam 2: The Structure of DNA

In practice, the unit of length used for DNA is the kilobase pair, which corresponds to

Most sequence-specific DNA binding proteins interact with

You have isolated a DNA molecule that is 12,200 base pairs in length. How many kilobase pairs is the DNA molecule?

A Hoogsteen base pair differs from a Watson-Crick base pair by

Explain how "hydrophobic bonding" stabilizes double-stranded DNA in aqueous solution.

Lowering the salt concentration of a DNA solution

Which statement is not true about DNA supercoiling?

In the two common Watson-Crick base pairs of DNA

Edwin Chargaff found that the base composition of DNA, defined as the "percent G+C,"

Watson and Crick noted that DNA's structure is interesting because it suggested a possible copying mechanism. What about DNA's structure facilitates copying?

Supercoiling is a form of DNA

DNA-mediated heredity derives from which part of its nucleotide building blocks?

The __________________ carries a message (the base sequence of DNA) in a form that can be read by DNA-binding proteins; the bases form __________________ with the side chains of amino acids of proteins.

Use a drawing to illustrate the principles of denaturation, renaturation, and nucleic acid hybridization.

Explain what is meant by the following descriptions of the DNA double helix: "polarity in each strand," and "the two strands are antiparallel."

DNA loop domains

Draw a typical DNA denaturation curve. Label the axes and point out the melting temperature (Tm). Indicate at what points all of the DNA is double-stranded and all of the DNA is single-stranded.

Briefly describe the two main types of interactions that provide chemical stability to the DNA double helix.

Friedreich's ataxia is a rare inherited neurological disease caused by a

Which statement about B-DNA is not true?

The Beginnings of Molecular Biology

The Versatility of RNA

Protein Structure and Folding

Genome Organization and Evolution

DNA Replication and Telomere Maintenance

DNA Repair Pathways

Recombinant Dna Technology and Molecular Cloning

Tools for Analyzing Gene Expression

Transcription in Bacteria

Transcription in Eukaryotes

Epigenetic Mechanisms of Gene Regulation

RNA Processing and Post-Transcriptional Gene Regulation

The Mechanism of Translation

Genetically Modified Organisms: Use in Basic and Applied Research

Genome Analysis: DNA Typing, Genomics, and Beyond

Medical Molecular Biology

Filters

The carries a message (the base sequence of DNA) in a form that can be read by DNA-binding proteins; the bases form with the side chains of amino acids of proteins.

Draw a typical DNA denaturation curve. Label the axes and point out the melting temperature (T_m). Indicate at what points all of the DNA is double-stranded and all of the DNA is single-stranded.