Exam 4: Bacterial Culture, Growth, and Development

You have isolated a bacterium from the rumen of a cow and wish to know how it transports glucose into the cell. You perform an experiment that shows uptake of radioactively labeled glucose is equal when the organism is grown in media in the presence and absence of inhibitors of energy production. What is the mechanism of glucose transport in this cell?

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Question 1

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Why would an organism go through the process of sporulation? How might endospores cause problems in the food industry?

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When nutrients are not as readily available, the starvation response of some organisms results in the formation of a dormant endospore. Because endospores are resistant to heat and desiccation, if foods are not properly sterilized, endospores could survive in the food, then germinate and cause food spoilage or food-borne illness.

How do scientists know of the existence of "unculturable" organisms?

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They know by using molecular biology tools such as PCR and gene sequencing. PCR is used to screen environmental samples for the presence of specific DNA sequences. Comparing DNA sequences of the PCR products with sequences of known organisms reveals insights into the physiology of undiscovered organisms and relationships with known organisms.

Which forms are NOT made by Streptomyces?

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Question 4

If you determined the number of cells in a sample using viable plate counts and optical density measurements, why would the results from the two methods be so different?

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Question 5

What type of medium and conditions would you need to have in order to select for photoautotrophs?

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Question 6

Compare and contrast the difference between electrogenic and electroneutral coupled transport and give an example of each.

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Question 7

Which of the following prokaryotic transport systems does NOT require the use of cellular energy to bring compounds into the cells?

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Question 8

What type of growth pattern is represented here: 5 * 10³; 1 * 10⁴; 2 * 10⁴; 4 * 10⁴; 8 * 10⁴?

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Question 9

Discuss some disadvantages of viable counts of microorganisms.

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Question 10

Siderophore-iron complexes enter cells with the help of

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Question 11

The primary goal of biofilms is thought to be

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Question 12

The majority of nitrogen in soil and marine environments is fixed by

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Question 13

Why is iron so often a limiting nutrient, when it seems to be so abundant in the environment, and how can organisms overcome this problem?

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Question 14

All of Earth's life-forms are based on

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Question 15

Cyanobacterial heterocysts function to fix atmospheric

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Question 16

What is the classic approach for finding new antibiotics?

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Question 17

When studying the metabolism of an organism, why is it better to grow organisms in a defined synthetic medium instead of a complex medium?

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Question 18

The most widely used solidifying agent is ________, which is derived from seaweed.

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Question 19

During biofilm formation, as more and more cells bind to the surface, they begin sending signals to each other in a process called

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Question 20

Showing 1 - 20 of 70

Exam 4: Bacterial Culture, Growth, and Development

Why would an organism go through the process of sporulation? How might endospores cause problems in the food industry?

How do scientists know of the existence of "unculturable" organisms?

Which forms are NOT made by Streptomyces?

If you determined the number of cells in a sample using viable plate counts and optical density measurements, why would the results from the two methods be so different?

What type of medium and conditions would you need to have in order to select for photoautotrophs?

Compare and contrast the difference between electrogenic and electroneutral coupled transport and give an example of each.

Which of the following prokaryotic transport systems does NOT require the use of cellular energy to bring compounds into the cells?

What type of growth pattern is represented here: 5 * 10³; 1 * 10⁴; 2 * 10⁴; 4 * 10⁴; 8 * 10⁴?

Discuss some disadvantages of viable counts of microorganisms.

Siderophore-iron complexes enter cells with the help of

The primary goal of biofilms is thought to be

The majority of nitrogen in soil and marine environments is fixed by

Why is iron so often a limiting nutrient, when it seems to be so abundant in the environment, and how can organisms overcome this problem?

All of Earth's life-forms are based on

Cyanobacterial heterocysts function to fix atmospheric

What is the classic approach for finding new antibiotics?

When studying the metabolism of an organism, why is it better to grow organisms in a defined synthetic medium instead of a complex medium?

The most widely used solidifying agent is ________, which is derived from seaweed.

During biofilm formation, as more and more cells bind to the surface, they begin sending signals to each other in a process called

Microbial Life: Origin and Discovery

Observing the Microbial Cell

Cell Structure and Function

Environmental Influences and Control of Microbial Growth

Viruses

Genomes and Chromosomes

Transcription, Translation, and Bioinformatics

Gene Transfer, Mutations, and Genome Evolution

Molecular Regulation

Viral Molecular Biology

Biotechniques and Synthetic Biology

Energetics and Catabolism

Electron Flow in Organotrophy, Lithotrophy, and Phototrophy

Biosynthesis

Food and Industrial Microbiology

Origins and Evolution

Bacterial Diversity

Archaeal Diversity

Eukaryotic Diversity

Microbial Ecology

Microbes in Global Elemental Cycles

Human Microbiota and Innate Immunity

The Adaptive Immune Response

Microbial Pathogenesis

Microbial Diseases

Antimicrobial Therapy

Clinical Microbiology and Epidemiology

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