Question 1

When light bends as it passes through an object,________ of the light has occurred.

Accepted Answer

A) reflection 
B) absorption 
C) transmission 
D) refraction 
A) reflection 
B) absorption 
C) transmission 
D) refraction D

Question 2

Atomic force microscope:

Accepted Answer

A) allows 3 dimensional imaging and measurement of structures as small as nucleotides in DNA 
B) is not yet capable of measuring small forces 
C) involves ultraviolet light exciting molecules so that they release light of a longer wavelength 
D) has a special condenser and objective lens 
A) allows 3 dimensional imaging and measurement of structures as small as nucleotides in DNA 
B) is not yet capable of measuring small forces 
C) involves ultraviolet light exciting molecules so that they release light of a longer wavelength 
D) has a special condenser and objective lens A

Question 3

Which of the following statements about resolution is true?

Accepted Answer

A) Resolution refers to the ability of a lens to distinguish adjacent objects. 
B) With regard to light, resolution means the same thing as wavelength. 
C) Resolution refers to a microscope's ability to magnify objects. 
D) Resolution is equal to the distance between two adjacent crests of a wave. 
A) Resolution refers to the ability of a lens to distinguish adjacent objects. 
B) With regard to light, resolution means the same thing as wavelength. 
C) Resolution refers to a microscope's ability to magnify objects. 
D) Resolution is equal to the distance between two adjacent crests of a wave. A

Question 4

During microscopic observation of a specimen,the amount of light that is allowed to pass through the specimen is controlled by the:

Accepted Answer

A) condenser 
B) objective lens 
C) iris diaphragm 
D) ocular lens 
A) condenser 
B) objective lens 
C) iris diaphragm 
D) ocular lens

Question 5

A parfocal microscope:

Accepted Answer

A) has more than one source of illumination 
B) has both coarse and fine focusing adjustment 
C) accentuates small differences in the refractive index of intracellular structures 
D) allows for specimens to remain in focus when changing between magnification 
A) has more than one source of illumination 
B) has both coarse and fine focusing adjustment 
C) accentuates small differences in the refractive index of intracellular structures 
D) allows for specimens to remain in focus when changing between magnification

Question 6

Which is a false statement about light microscopy?

Accepted Answer

A) A dark-field microscope produces bright images against a dark background. 
B) A phase contrast microscope gives 3-dimensional images. 
C) Fluorescent antibody staining cannot determine whether a foreign organism such as a microbe is present in a specimen. 
D) A Nomarski microscope produces much higher resolution than the standard phase-contrast microscope. 
A) A dark-field microscope produces bright images against a dark background. 
B) A phase contrast microscope gives 3-dimensional images. 
C) Fluorescent antibody staining cannot determine whether a foreign organism such as a microbe is present in a specimen. 
D) A Nomarski microscope produces much higher resolution than the standard phase-contrast microscope.

Question 7

Which of the following statements about preparing a light microscope specimen is false?

Accepted Answer

A) Organisms must be heat fixed before being viewed in a hanging drop slide. 
B) Smears are loopfuls of medium spread on the surface of a glass slide. 
C) Wet mount preparations give good views of microbial mobility. 
D) The depth of a smear affects the results; if too thin you may find no organisms. 
A) Organisms must be heat fixed before being viewed in a hanging drop slide. 
B) Smears are loopfuls of medium spread on the surface of a glass slide. 
C) Wet mount preparations give good views of microbial mobility. 
D) The depth of a smear affects the results; if too thin you may find no organisms.

Question 8

The total magnification of a specimen being viewed with a 10X ocular lens and a 40X objective lens is _____.

Accepted Answer

A) 4X 
B) 40X 
C) 400X 
D) 4000X 
A) 4X 
B) 40X 
C) 400X 
D) 4000X

Question 9

In order to make use of light for a microscopic examination of an object the object must ________ or ________ light.

Accepted Answer

A) absorb, luminesce 
B) transmit, absorb 
C) transmit, reflect 
D) reflect, absorb 
A) absorb, luminesce 
B) transmit, absorb 
C) transmit, reflect 
D) reflect, absorb

Question 10

The counterstain in the endospore stain is _____.

Accepted Answer

A) malachite green 
B) crystal violet 
C) safranin 
D) methylene blue 
A) malachite green 
B) crystal violet 
C) safranin 
D) methylene blue

Question 11

Why is diffraction a problem for microscopy?

Accepted Answer

A) The lens acts as a large aperture through which light must pass. 
B) The small size of higher-power lenses causes severe diffraction. 
C) The loss of light results in blurred images. 
D) Diffraction of light is useful when using an oil immersion lens to view objects. 
A) The lens acts as a large aperture through which light must pass. 
B) The small size of higher-power lenses causes severe diffraction. 
C) The loss of light results in blurred images. 
D) Diffraction of light is useful when using an oil immersion lens to view objects.

Question 12

The advent of the electron microscope allowed ________ to be viewed for the first time.

Accepted Answer

A) protozoa 
B) bacteria 
C) viruses 
D) algae 
A) protozoa 
B) bacteria 
C) viruses 
D) algae

Question 13

A microscope in which light rays pass directly through a specimen is a ________ microscope.

Accepted Answer

A) bright field 
B) dark field 
C) phase-contrast 
D) Nomarski 
A) bright field 
B) dark field 
C) phase-contrast 
D) Nomarski

Question 14

The lens closest to the slide during a microscopic examination is the _____.

Accepted Answer

A) ocular 
B) objective 
C) condenser 
D) compound 
A) ocular 
B) objective 
C) condenser 
D) compound

Question 15

A simple stain:

Accepted Answer

A) uses only a single dye. 
B) requires only one step to stain a slide. 
C) distinguishes between two different parts of an organism. 
D) is composed of an equal balance of acidic and basic dyes. 
A) uses only a single dye. 
B) requires only one step to stain a slide. 
C) distinguishes between two different parts of an organism. 
D) is composed of an equal balance of acidic and basic dyes.

Question 16

When given a microorganism to identify,which of the following would be useful?

Accepted Answer

A) A Gram stain 
B) A transmission electron micrograph 
C) Biochemical and genetic characteristics 
D) All of the above 
A) A Gram stain 
B) A transmission electron micrograph 
C) Biochemical and genetic characteristics 
D) All of the above

Question 17

Which of the following stains has been used on this microorganism?

Accepted Answer

A) Flagellar stain 
B) Capsule stain 
C) Endospore stain 
D) No stain 
A) Flagellar stain 
B) Capsule stain 
C) Endospore stain 
D) No stain

Question 18

Suitable stains for use in the Ziehl-Neelsen acid-fast stain are _____.

Accepted Answer

A) crystal violet and eosin 
B) carbolfuschin and methylene blue 
C) carbolfuschin and safranin 
D) safranin and methylene blue 
A) crystal violet and eosin 
B) carbolfuschin and methylene blue 
C) carbolfuschin and safranin 
D) safranin and methylene blue

Question 19

When light rays pass into an object but do not emerge,________ has taken place.

Accepted Answer

A) reflection 
B) absorption 
C) refraction 
D) transmission 
A) reflection 
B) absorption 
C) refraction 
D) transmission

Question 20

Transmission electron microscopes have a maximum magnification of _____.

Accepted Answer

A) 1,000X 
B) 100,000X 
C) 500,000X 
D) 1,000,000X 
A) 1,000X 
B) 100,000X 
C) 500,000X 
D) 1,000,000X

Exam 3: Microscopy and Staining

When light bends as it passes through an object,________ of the light has occurred.

Atomic force microscope:

Which of the following statements about resolution is true?

During microscopic observation of a specimen,the amount of light that is allowed to pass through the specimen is controlled by the:

A parfocal microscope:

Which is a false statement about light microscopy?

Which of the following statements about preparing a light microscope specimen is false?

The total magnification of a specimen being viewed with a 10X ocular lens and a 40X objective lens is _____.

In order to make use of light for a microscopic examination of an object the object must ________ or ________ light.

The counterstain in the endospore stain is _____.

Why is diffraction a problem for microscopy?

The advent of the electron microscope allowed ________ to be viewed for the first time.

A microscope in which light rays pass directly through a specimen is a ________ microscope.

The lens closest to the slide during a microscopic examination is the _____.

A simple stain:

When given a microorganism to identify,which of the following would be useful?

Which of the following stains has been used on this microorganism?

Suitable stains for use in the Ziehl-Neelsen acid-fast stain are _____.

When light rays pass into an object but do not emerge,________ has taken place.

Transmission electron microscopes have a maximum magnification of _____.

Scope and History of Microbiology

Fundamentals of Chemistry

Characteristics of Prokaryotic and Eukaryotic Cells

Essential Concepts of Metabolism

Growth and Culturing of Bacteria

Microbial Genetics

Gene Transfer and Genetic Engineering

An Introduction to Taxonomy: the Bacteria

Viruses

Eukaryotic Microorganisms and Parasites

Sterilization and Disinfection

Antimicrobial Therapy

Host-Microbe Relationships and Disease Processes

Epidemiology and Nosocomial Infections

Innate Host Defenses

Immunology I: Basic Principles of Adaptive Immunity and Immunization

Immunology Ii: Immunological Disorders and Tests

Diseases of the Skin and Eyes; Wounds and Bites

Urogenital and Sexually Transmitted Diseases

Diseases of the Respiratory System

Oral and Gastrointestinal Diseases

Cardiovascular, lymphatic and Systemic Disease

Diseases of the Nervous System

Environmental Microbiology

Applied Microbiology

Filters

In order to make use of light for a microscopic examination of an object the object must or light.