Question 1

This structure converges the light beams so they pass through the specimen.

Accepted Answer

A) A 
B) B 
C) C 
D) D 
A) A 
B) B 
C) C 
D) D

Question 2

Bacterial capsules can best be visualized by _______ staining.

Accepted Answer

A) flagellar 
B) crystal violet 
C) negative 
D) mordant 
A) flagellar 
B) crystal violet 
C) negative 
D) mordant

Question 3

If the step involving iodine were left out of a Gram stain,which of the following would best describe the results?

Accepted Answer

A) Gram negative cells would look Gram positive. 
B) Gram positive cells would look Gram negative. 
C) All rods would be pink, all cocci purple. 
D) All cells would be purple. 
A) Gram negative cells would look Gram positive. 
B) Gram positive cells would look Gram negative. 
C) All rods would be pink, all cocci purple. 
D) All cells would be purple.

Question 4

Which stain would be the best choice for detecting mycobacterium (the bacteria responsible for tuberculosis and leprosy)?

Accepted Answer

A) simple stain 
B) endospore stain 
C) acid-fast stain 
D) Gram stain 
A) simple stain 
B) endospore stain 
C) acid-fast stain 
D) Gram stain

Question 5

What statement about microscopy and staining techniques is false?

Accepted Answer

A) Many species look identical under the microscope. 
B) Staining and microscopic examination are usually all that is need to identify a microorganism. 
C) Microscopes are of little use unless the specimens are prepared properly. 
D) The degree of contrast is equally important as resolution and magnification. 
A) Many species look identical under the microscope. 
B) Staining and microscopic examination are usually all that is need to identify a microorganism. 
C) Microscopes are of little use unless the specimens are prepared properly. 
D) The degree of contrast is equally important as resolution and magnification.

Question 6

Compare and contrast light microscopy and electron microscopy.Be sure to include how they work and what they can see (or the extent of their total magnification).

Accepted Answer

Light microscopy uses visible wavelength

Question 7

The lens closest to your eyes during a microscopic examination is the _____.

Accepted Answer

A) ocular 
B) objective 
C) condenser 
D) compound 
A) ocular 
B) objective 
C) condenser 
D) compound

Question 8

What is the Gram stain? What does it distinguish?

Accepted Answer

Gram staining is based on the ability of

Question 9

What is true about the index of refraction?

Accepted Answer

A) If light rays are taken up by the object than it has a high index of refraction. 
B) Refraction measures the frequency of the light as it reflects from a material. 
C) Oil immersion lenses increase the problem of refraction. 
D) Light will bend as it passes through two substances with different indices of refraction. 
A) If light rays are taken up by the object than it has a high index of refraction. 
B) Refraction measures the frequency of the light as it reflects from a material. 
C) Oil immersion lenses increase the problem of refraction. 
D) Light will bend as it passes through two substances with different indices of refraction.

Question 10

Which type of staining results in a clear object being viewed against a dark background?

Accepted Answer

A) Simple stain 
B) Negative stain 
C) Endospore stain 
D) Flagellar stain 
A) Simple stain 
B) Negative stain 
C) Endospore stain 
D) Flagellar stain

Question 11

Which of the following is not a differential stain?

Accepted Answer

A) Gram stain 
B) Schaeffer-Fulton 
C) acid-fast stain 
D) flagellar stain 
A) Gram stain 
B) Schaeffer-Fulton 
C) acid-fast stain 
D) flagellar stain

Question 12

The best electron microscopes have a resolution of _____ nm.

Accepted Answer

A) 0.1 
B) 1 
C) 10 
D) 100 
A) 0.1 
B) 1 
C) 10 
D) 100

Question 13

When light passes through an object,________ of the light has occurred.

Accepted Answer

A) reflection 
B) absorption 
C) transmission 
D) fluorescence 
A) reflection 
B) absorption 
C) transmission 
D) fluorescence

Question 14

Would light of a shorter or longer wavelength give you better resolution when using a microscope? Why? Would a light microscope that had a total magnification of 500X give you better resolution as one that has a magnification of 100X? Why?

Accepted Answer

Light of a shorter wavelength would give

Question 15

Electron microscopes:

Accepted Answer

A) that are scanning have better resolution than those that are transmission 
B) are much more expensive and take up more space than light microscopes 
C) can use the same preparations of specimens that have been prepared for viewing with a light microscope 
D) have a resolving power approximately 10 times better than the best light microscope 
A) that are scanning have better resolution than those that are transmission 
B) are much more expensive and take up more space than light microscopes 
C) can use the same preparations of specimens that have been prepared for viewing with a light microscope 
D) have a resolving power approximately 10 times better than the best light microscope

Question 16

The technique that involves the evaporation of water from a frozen and fractured specimen is called:

Accepted Answer

A) shadow casting 
B) freeze-etching 
C) heat fixation 
D) freeze-fracturing 
A) shadow casting 
B) freeze-etching 
C) heat fixation 
D) freeze-fracturing

Exam 3: Microscopy and Staining

This structure converges the light beams so they pass through the specimen.

Bacterial capsules can best be visualized by _______ staining.

If the step involving iodine were left out of a Gram stain,which of the following would best describe the results?

Which stain would be the best choice for detecting mycobacterium (the bacteria responsible for tuberculosis and leprosy)?

What statement about microscopy and staining techniques is false?

Compare and contrast light microscopy and electron microscopy.Be sure to include how they work and what they can see (or the extent of their total magnification).

The lens closest to your eyes during a microscopic examination is the _____.

What is the Gram stain? What does it distinguish?

What is true about the index of refraction?

Which type of staining results in a clear object being viewed against a dark background?

Which of the following is not a differential stain?

The best electron microscopes have a resolution of _____ nm.

When light passes through an object,________ of the light has occurred.

Would light of a shorter or longer wavelength give you better resolution when using a microscope? Why? Would a light microscope that had a total magnification of 500X give you better resolution as one that has a magnification of 100X? Why?

Electron microscopes:

The technique that involves the evaporation of water from a frozen and fractured specimen is called:

Scope and History of Microbiology

Fundamentals of Chemistry

Characteristics of Prokaryotic and Eukaryotic Cells

Essential Concepts of Metabolism

Growth and Culturing of Bacteria

Microbial Genetics

Gene Transfer and Genetic Engineering

An Introduction to Taxonomy: the Bacteria

Viruses

Eukaryotic Microorganisms and Parasites

Sterilization and Disinfection

Antimicrobial Therapy

Host-Microbe Relationships and Disease Processes

Epidemiology and Nosocomial Infections

Innate Host Defenses

Immunology I: Basic Principles of Adaptive Immunity and Immunization

Immunology Ii: Immunological Disorders and Tests

Diseases of the Skin and Eyes; Wounds and Bites

Urogenital and Sexually Transmitted Diseases

Diseases of the Respiratory System

Oral and Gastrointestinal Diseases

Cardiovascular, lymphatic and Systemic Disease

Diseases of the Nervous System

Environmental Microbiology

Applied Microbiology

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